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Despite great progress on schistosomiasis control over the past 50 years in China, <jats:italic>S. japonicum<\/jats:italic> transmission still occurs in certain endemic regions, which causes significant public health problems and enormous economic losses. During different life stages, parasites are able to survive dramatic osmolality changes between its vector, fresh water, and mammal host. However, the molecular mechanism of parasite osmoregulation remains unknown. To address this challenging question, we report the first cloning of an <jats:italic>S. japonicum<\/jats:italic> aquaglyceroporin (<jats:italic>Sj<\/jats:italic>AQP) from an isolate from Jiangsu province, China. Expressing <jats:italic>Sj<\/jats:italic>AQP in <jats:italic>Xenopus<\/jats:italic> oocytes facilitated the permeation of water, glycerol, and urea. The water permeability of <jats:italic>Sj<\/jats:italic>AQP was inhibited by 1\u2009mM HgCl<jats:sub>2<\/jats:sub>, 3\u2009mM tetraethylammonium, 1\u2009mM ZnCl<jats:sub>2<\/jats:sub>, and 1\u2009mM CuSO<jats:sub>4<\/jats:sub>. <jats:italic>Sj<\/jats:italic>AQP was constitutively expressed throughout the <jats:italic>S. japonicum<\/jats:italic> life cycle, including in the egg, miracidia, cercaria, and adult stages. The highest expression was detected during the infective cercaria stage. Our results suggest that <jats:italic>Sj<\/jats:italic>AQP plays a role in osmoregulation throughout the <jats:italic>S. japonicum<\/jats:italic> life cycle, especially during cercariae transformation, which enables parasites to survive osmotic challenges.<\/jats:p>","DOI":"10.1038\/srep35030","type":"journal-article","created":{"date-parts":[[2016,10,13]],"date-time":"2016-10-13T09:01:33Z","timestamp":1476349293000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Cloning and in vitro characterization of a Schistosoma japonicum aquaglyceroporin that functions in osmoregulation"],"prefix":"10.1038","volume":"6","author":[{"given":"Yuzheng","family":"Huang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wuguang","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunrong","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaijiang","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kun Connie","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,10,13]]},"reference":[{"key":"BFsrep35030_CR1","doi-asserted-by":"publisher","first-page":"819","DOI":"10.1186\/s12889-016-3494-y","volume":"16","author":"J Mwai","year":"2016","unstructured":"Mwai, J., Njenga, S. & Barasa, M. 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