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Seipin-deficient (\n                    <jats:italic>Bscl2<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    ) mice display an almost total loss of white adipose tissue (WAT) with residual brown adipose tissue (BAT). Previous cellular studies have shown that seipin deficiency alters white adipocyte differentiation. In this study, we aimed to decipher the consequences of seipin deficiency in BAT. Using a brown adipocyte cell-line, we show that seipin knockdown had very little effect on adipocyte differentiation without affecting insulin sensitivity and oxygen consumption. However, when submitted to cold acclimation or chronic \u03b23 agonist treatment,\n                    <jats:italic>Bscl2<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice displayed altered thermogenic capacity, despite several signs of BAT remodeling. Under cold activation,\n                    <jats:italic>Bscl2<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice were able to maintain their body temperature when fed\n                    <jats:italic>ad libitum,<\/jats:italic>\n                    but not under short fasting. At control temperature (\n                    <jats:italic>i.e.<\/jats:italic>\n                    21\u2009\u00b0C), fasting worsened\n                    <jats:italic>Bscl2<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    BAT properties. Finally,\n                    <jats:italic>Bscl2<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    BAT displayed obvious signs of insulin resistance. Our results in these lipodystrophic mice strongly suggest that BAT activity relies on WAT as an energetic substrate provider and adipokine-producing organ. Therefore, the WAT\/BAT dialogue is a key component of BAT integrity in guaranteeing its response to insulin and cold-activated adrenergic signals.\n                  <\/jats:p>","DOI":"10.1038\/srep35487","type":"journal-article","created":{"date-parts":[[2016,10,17]],"date-time":"2016-10-17T07:17:07Z","timestamp":1476688627000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Seipin deficiency alters brown adipose tissue thermogenesis and insulin sensitivity in a non-cell autonomous mode"],"prefix":"10.1038","volume":"6","author":[{"given":"L.","family":"Dollet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Magr\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Joubert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Le May","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Ayer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Arnaud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Pecqueur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Blouin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Cariou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Prieur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,10,17]]},"reference":[{"key":"BFsrep35487_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1159\/000316893","volume":"19","author":"J Capeau","year":"2010","unstructured":"Capeau, J., Magr\u00e9, J., Caron-Debarle, M., Lagathu, C. et al. 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