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However, whether NK3R expression can serve as a regulatory target at the pituitary level and contribute to NKB interactions with other SL\u03b1 regulators is still unclear. In current study, using grass carp pituitary cells as a model, we have a novel finding that co-treatment of SL\u03b1\/SL\u03b2 with carp TAC3 gene products, could induce a noticeable enhancement in SL\u03b1 mRNA expression and these potentiating effects occurred with a parallel rise in NK3R transcript level after SL\u03b1\/SL\u03b2 treatment. Interestingly, the stimulatory effects of SL\u03b1\/SL\u03b2 on NK3R gene expression could be further potentiated by co-treatment with IGF-I\/-II and simultaneous exposure of carp pituitary cells to SL\u03b1\/SL\u03b2 and IGF-I\/-II in the presence of TAC3 gene products was found to markedly elevated SL\u03b1 mRNA expression (20 fold increase) and this synergistic stimulation was mediated by cAMP\/PKA-, PLC\/PKC- and Ca<jats:sup>2+<\/jats:sup> -dependent cascades functionally coupled with NK3R activation. These findings suggest that local release of SL\u03b1 via functional interactions with IGF-I\/-II and TAC3\/NK3R system may constitute a potent stimulatory signal for SL\u03b1 gene expression in the carp pituitary via up-regulation of NK3R expression.<\/jats:p>","DOI":"10.1038\/srep36102","type":"journal-article","created":{"date-parts":[[2016,10,27]],"date-time":"2016-10-27T10:30:36Z","timestamp":1477564236000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Novel Functional Role of NK3R Expression in the Potentiating Effects on Somatolactin \u03b1 Autoregulation in grass carp pituitary cells"],"prefix":"10.1038","volume":"6","author":[{"given":"Guangfu","family":"Hu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mulan","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anderson On Lam","family":"Wong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,10,27]]},"reference":[{"key":"BFsrep36102_CR1","doi-asserted-by":"crossref","first-page":"4330","DOI":"10.1073\/pnas.87.11.4330","volume":"87","author":"M Ono","year":"1990","unstructured":"Ono, M. et al. cDNA cloning of somatolactin, a pituitary protein related to growth hormone and prolactin. 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