{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T04:58:57Z","timestamp":1768798737505,"version":"3.49.0"},"reference-count":52,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T00:00:00Z","timestamp":1478476800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T00:00:00Z","timestamp":1478476800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Isoflavonoids have been largely studied due to their distinct biological activities identified thus far. Herein, we evaluated the activity of neovestitol, an isoflavonoid isolated from Brazilian red propolis, in acute and chronic inflammation. As for acute inflammation, we found that neovestitol reduced neutrophil migration, leukocyte rolling and adhesion, as well as expression of ICAM-1 in the mesenteric microcirculation during lipopolysaccharide-induced acute peritonitis. No changes were observed in the levels of TNF-\u03b1, CXCL1\/KC and CXCL2\/MIP-2 upon pretreatment with neovestitol. The administration of an inducible nitric oxide synthase (iNOS) inhibitor abolished the inhibitory effects of neovestitol in neutrophil migration and ICAM-1 expression. Nitrite levels increased upon treatment with neovestitol. No effects of neovestitol were observed on the chemotaxis of neutrophils <jats:italic>in vitro<\/jats:italic>. As for chronic inflammation, neovestitol also reduced the clinical score and joint damage in a collagen-induced arthritis model. There was no change in the frequency of IL-17-producing TCD4+ cells. In addition, pretreatment with neovestitol reduced the levels of IL-6. These results demonstrate a potential anti-inflammatory activity of neovestitol, which may be useful for therapeutic purposes and\/or as a nutraceutical.<\/jats:p>","DOI":"10.1038\/srep36401","type":"journal-article","created":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T11:21:42Z","timestamp":1478517702000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Neovestitol, an isoflavonoid isolated from Brazilian red propolis, reduces acute and chronic inflammation: involvement of nitric oxide and IL-6"],"prefix":"10.1038","volume":"6","author":[{"given":"Marcelo","family":"Franchin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David F.","family":"Col\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos G.","family":"da Cunha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda V. S.","family":"Castanheira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 L. L.","family":"Saraiva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Bueno-Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Severino M.","family":"Alencar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thiago M.","family":"Cunha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro L.","family":"Rosalen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,11,7]]},"reference":[{"key":"BFsrep36401_CR1","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1189\/jlb.0811402","volume":"91","author":"CD Sadik","year":"2012","unstructured":"Sadik, C. D. & Luster, A. D. Lipid-cytokine-chemokine cascades orchestrate leukocyte recruitment in inflammation. J. Leukoc. Biol. 91, 207\u2013215 (2012).","journal-title":"J. Leukoc. Biol."},{"key":"BFsrep36401_CR2","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/S1471-4906(02)00013-3","volume":"24","author":"G Kaplanski","year":"2003","unstructured":"Kaplanski, G., Marin, V., Montero-Julian, F., Mantovani, A. & Farnarier, C. IL-6: a regulator of the transition from neutrophil to monocyte recruitment during inflammation. Trends Immunol. 24, 25\u201329 (2003).","journal-title":"Trends Immunol."},{"key":"BFsrep36401_CR3","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.1084\/jem.20122220","volume":"210","author":"A Mocsai","year":"2013","unstructured":"Mocsai, A. Diverse novel functions of neutrophils in immunity, inflammation, and beyond. J. Exp. Med. 210, 1283\u20131299 (2013).","journal-title":"J. Exp. Med."},{"key":"BFsrep36401_CR4","doi-asserted-by":"publisher","first-page":"2205","DOI":"10.1056\/NEJMra1004965","volume":"365","author":"IB McInnes","year":"2011","unstructured":"McInnes, I. B. & Schett, G. The pathogenesis of rheumatoid arthritis. N. Engl. J. Med. 365, 2205\u20132219 (2011).","journal-title":"N. Engl. J. Med."},{"key":"BFsrep36401_CR5","doi-asserted-by":"publisher","first-page":"S1","DOI":"10.1016\/S0367-326X(02)00185-5","volume":"73","author":"S Castaldo","year":"2002","unstructured":"Castaldo, S. & Capasso, F. Propolis, an old remedy used in modern medicine. Fitoterapia. 73, S1\u2013S6 (2002).","journal-title":"Fitoterapia."},{"key":"BFsrep36401_CR6","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1111\/jocd.12123","volume":"14","author":"N Saewan","year":"2015","unstructured":"Saewan, N. & Jimtaisong, A. Natural products as photoprotection. J. Cosmet. Dermatol. 14, 47\u201363 (2015).","journal-title":"J. Cosmet. Dermatol."},{"key":"BFsrep36401_CR7","doi-asserted-by":"publisher","first-page":"9","DOI":"10.4314\/ejhs.v25i1.3","volume":"25","author":"S Mohsin","year":"2015","unstructured":"Mohsin, S., Manohar, B., Rajesh, S. & Asif, Y. The effects of a dentifrice containing propolis on Mutans Streptococci: a clinico-microbiological study. Ethiop. J. Health Sci. 25, 9\u201316 (2015).","journal-title":"Ethiop. J. Health Sci."},{"key":"BFsrep36401_CR8","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1016\/j.ijfoodmicro.2015.03.020","volume":"205","author":"A Luis-Villaroya","year":"2015","unstructured":"Luis-Villaroya, A. et al. Bioactive properties of a propolis-based dietary supplement and its use in combination with mild heat for apple juice preservation. Int. J. Food Microbiol. 205, 90\u201397 (2015).","journal-title":"Int. J. Food Microbiol."},{"key":"BFsrep36401_CR9","doi-asserted-by":"publisher","first-page":"2502","DOI":"10.1021\/jf011432b","volume":"50","author":"YK Park","year":"2002","unstructured":"Park, Y. K., Alencar, S. M. & Aguiar, C. L. Botanical origin and chemical composition of Brazilian propolis. J. Agric. Food Chem. 50, 2502\u20132506 (2002).","journal-title":"J. Agric. Food Chem."},{"key":"BFsrep36401_CR10","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1093\/ecam\/nem059","volume":"5","author":"BB Silva","year":"2008","unstructured":"Silva, B. B. et al. Chemical composition and botanical origin of red propolis, a new type of brazilian propolis. Evid. Based. Complement. Alternat. 5, 313\u2013316 (2008).","journal-title":"Evid. Based. Complement. Alternat."},{"key":"BFsrep36401_CR11","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1016\/j.jep.2007.06.005","volume":"113","author":"SM Alencar","year":"2007","unstructured":"Alencar, S. M. et al. Chemical composition and biological activity of a new type of Brazilian propolis: red propolis. J. Ethnopharmacol. 113, 278\u2013283 (2007).","journal-title":"J. Ethnopharmacol."},{"key":"BFsrep36401_CR12","doi-asserted-by":"publisher","first-page":"1233","DOI":"10.1080\/08927014.2013.834050","volume":"29","author":"B Bueno-Silva","year":"2013","unstructured":"Bueno-Silva, B. et al. Effect of neovestitol-vestitol containing Brazilian red propolis on accumulation of biofilm in vitro and development of dental caries in vivo. Biofouling. 29, 1233\u20131242 (2013).","journal-title":"Biofouling."},{"key":"BFsrep36401_CR13","doi-asserted-by":"publisher","first-page":"4546","DOI":"10.1021\/jf305468f","volume":"61","author":"B Bueno-Silva","year":"2013","unstructured":"Bueno-Silva, B. et al. Anti-inflammatory and antimicrobial evaluation of neovestitol and vestitol isolated from Brazilian red propolis. J. Agric. Food Chem. 61, 4546\u20134550 (2013).","journal-title":"J. Agric. Food Chem."},{"key":"BFsrep36401_CR14","doi-asserted-by":"publisher","first-page":"692","DOI":"10.1038\/nri3908","volume":"15","author":"D Vestweber","year":"2015","unstructured":"Vestweber, D. How leukocytes cross the vascular endothelium. Nat. Rev. Immunol. 15, 692\u2013704 (2015).","journal-title":"Nat. Rev. Immunol."},{"key":"BFsrep36401_CR15","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/j.niox.2006.02.004","volume":"15","author":"D Dal Secco","year":"2006","unstructured":"Dal Secco, D. et al. Nitric oxide inhibits neutrophil migration by a mechanism dependent on ICAM-1: role of soluble guanylate cyclase. Nitric Oxide. 15, 77\u201386 (2006).","journal-title":"Nitric Oxide."},{"key":"BFsrep36401_CR16","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/j.niox.2003.11.001","volume":"9","author":"D Dal Secco","year":"2003","unstructured":"Dal Secco, D., Paron, J. A., de Oliveira, S. H., Ferreira, S. H. & Cunha, F. Q. Neutrophil migration in inflammation: nitric oxide inhibits rolling, adhesion and induces apoptosis. Nitric Oxide. 9, 153\u2013164 (2003).","journal-title":"Nitric Oxide."},{"key":"BFsrep36401_CR17","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1038\/nri3399","volume":"13","author":"E Kolaczkowska","year":"2013","unstructured":"Kolaczkowska, E. & Kubes, P. Nat. Rev. Neutrophil recruitment and function in health and inflammation. Nat. Rev. Immunol. 13, 159\u2013175 (2013).","journal-title":"Nat. Rev. Immunol."},{"key":"BFsrep36401_CR18","doi-asserted-by":"publisher","first-page":"668","DOI":"10.1016\/j.autrev.2013.12.004","volume":"13","author":"M Noack","year":"2014","unstructured":"Noack, M. & Miossec, P. Autoimmunity Reviews Th17 and regulatory T cell balance in autoimmune and inflammatory diseases. Autoimmun. Rev. 13, 668\u2013677 (2014).","journal-title":"Autoimmun. Rev."},{"key":"BFsrep36401_CR19","doi-asserted-by":"publisher","first-page":"727","DOI":"10.1038\/icb.2010.26","volume":"88","author":"M Bandara","year":"2010","unstructured":"Bandara, M. et al. Topical isoflavonoids reduce experimental cutaneous inflammation in mice. Immunol. Cell Biol. 88, 727\u2013733 (2010).","journal-title":"Immunol. Cell Biol."},{"key":"BFsrep36401_CR20","doi-asserted-by":"publisher","first-page":"954","DOI":"10.1021\/acs.jnatprod.5b00938","volume":"79","author":"M Franchin","year":"2016","unstructured":"Franchin, M. et al. Vestitol Isolated from Brazilian Red Propolis Inhibits Neutrophils Migration in the Inflammatory Process: Elucidation of the Mechanism of Action. J. Nat. Prod. 79, 954\u2013960 (2016).","journal-title":"J. Nat. Prod."},{"key":"BFsrep36401_CR21","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1016\/j.jep.2015.07.022","volume":"173","author":"R Lima Cavendish","year":"2015","unstructured":"Lima Cavendish, R. et al. Antinociceptive and anti-inflammatory effects of Brazilian red propolis extract and formononetin in rodents. J. Ethnopharmacol. 173, 127\u2013133 (2015).","journal-title":"J. Ethnopharmacol."},{"key":"BFsrep36401_CR22","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.taap.2015.01.012","volume":"283","author":"AA Ganai","year":"2015","unstructured":"Ganai, A. A., Khan, A. A., Malik, Z. A. & Farooqi, H. Genistein modulates the expression of NF-kappaB and MAPK (p-38 and ERK1\/2), thereby attenuating d-Galactosamine induced fulminant hepatic failure in Wistar rats. Toxicol. Appl. Pharmacol. 283, 139\u2013146 (2015).","journal-title":"Toxicol. Appl. Pharmacol."},{"key":"BFsrep36401_CR23","doi-asserted-by":"publisher","first-page":"391","DOI":"10.3892\/ijmm.2014.2020","volume":"35","author":"WY Wu","year":"2015","unstructured":"Wu, W. Y. et al. Biochanin A attenuates LPS-induced pro-inflammatory responses and inhibits the activation of the MAPK pathway in BV2 microglial cells. Int. J. Mol. Med. 35, 391\u2013398 (2015).","journal-title":"Int. J. Mol. Med."},{"key":"BFsrep36401_CR24","doi-asserted-by":"publisher","first-page":"602","DOI":"10.1038\/ni.2921","volume":"15","author":"WM Nauseef","year":"2014","unstructured":"Nauseef, W. M. & Borregaard, N. Neutrophils at work. Nat. Immunol. 15, 602\u2013611 (2014).","journal-title":"Nat. Immunol."},{"key":"BFsrep36401_CR25","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1023\/A:1010927921018","volume":"25","author":"BM Tavares-Murta","year":"2001","unstructured":"Tavares-Murta, B. M., Machado, J. S., Ferreira, S. H. & Cunha F. Q. Nitric Oxide Mediates the Inhibition of Neutrophil Migration Induced by Systemic Administration of LPS. Inflammation. Inflammation. 25, 247\u2013253 (2001).","journal-title":"Inflammation."},{"key":"BFsrep36401_CR26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1155\/2010\/682879","volume":"2010","author":"M Iwata","year":"2010","unstructured":"Iwata, M., Suzuki, S., Asai, Y., Inoue, T. & Takagi, K. Involvement of nitric oxide in a rat model of carrageenin-induced pleurisy. Mediators Inflamm. 2010, 1\u201311 (2010).","journal-title":"Mediators Inflamm."},{"key":"BFsrep36401_CR27","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1002\/med.10001","volume":"22","author":"H Yusuf-Makagiansar","year":"2002","unstructured":"Yusuf-Makagiansar, H., Anderson, M. E., Yakovleva, T. V., Murray, J. S. & Siahaan, T. J. Inhibition of LFA-1\/ICAM-1 and VLA-4\/VCAM-1 as a therapeutic approach to inflammation and autoimmune diseases. Med. Res. Rev. 22, 146\u2013167 (2002).","journal-title":"Med. Res. Rev."},{"key":"BFsrep36401_CR28","doi-asserted-by":"publisher","first-page":"2128","DOI":"10.2174\/138161208785740225","volume":"14","author":"T Zimmerman","year":"2008","unstructured":"Zimmerman, T. & Blanco, F. J. Inhibitors targeting the LFA-1\/ICAM-1 cell-adhesion interaction: design and mechanism of action. Curr. Pharm. Des. 14, 2128\u20132139 (2008).","journal-title":"Curr. Pharm. Des."},{"key":"BFsrep36401_CR29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/1476-9255-8-7","volume":"8","author":"R Kacimi","year":"2011","unstructured":"Kacimi, R., Giffard, R. G. & Yenari, M. A. Endotoxin-activated microglia injure brain derived endothelial cells via NF-\u03baB, JAK-STAT and JNK stress kinase pathways. J. Inflamm. (Lond). 8, 1\u201315 (2011).","journal-title":"J. Inflamm. (Lond)."},{"key":"BFsrep36401_CR30","doi-asserted-by":"publisher","first-page":"878","DOI":"10.1016\/j.bbamcr.2011.01.034","volume":"1813","author":"J Scheller","year":"2011","unstructured":"Scheller, J., Chalaris, A., Schmidt-Arras, D. & Rose-John, S. The pro- and anti-inflammatory properties of the cytokine interleukin-6. Biochim. Biophys. Acta. 1813, 878\u2013888 (2011).","journal-title":"Biochim. Biophys. Acta."},{"key":"BFsrep36401_CR31","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/j.cytogfr.2011.02.003","volume":"22","author":"MF Neurath","year":"2011","unstructured":"Neurath, M. F. & Finotto, S. IL-6 signaling in autoimmunity, chronic inflammation and inflammation-associated cancer. Cytokine Growth Factor Rev. 22, 83\u201389 (2011).","journal-title":"Cytokine Growth Factor Rev."},{"key":"BFsrep36401_CR32","doi-asserted-by":"publisher","first-page":"3710","DOI":"10.1002\/art.24126","volume":"58","author":"M Fujimoto","year":"2008","unstructured":"Fujimoto, M. et al. Interleukin-6 blockade suppresses autoimmune arthritis in mice by the inhibition of inflammatory Th17 responses. Arthritis Rheum. 58, 3710\u20133719 (2008).","journal-title":"Arthritis Rheum."},{"key":"BFsrep36401_CR33","doi-asserted-by":"publisher","first-page":"2117","DOI":"10.1002\/1529-0131(199812)41:12<2117::AID-ART6>3.0.CO;2-P","volume":"41","author":"N Takagi","year":"1998","unstructured":"Takagi, N. et al. Blockage of interleukin-6 receptor ameliorates joint disease in murine collagen-induced arthritis. Arthritis Rheum. 41, 2117\u20132121 (1998).","journal-title":"Arthritis Rheum."},{"key":"BFsrep36401_CR34","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1177\/1759720X10378372","volume":"2","author":"S Srirangan","year":"2010","unstructured":"Srirangan, S. & Choy, E. H. The role of interleukin 6 in the pathophysiology of rheumatoid arthritis. Ther. Adv. Musculoskelet Dis. 2, 247\u2013256 (2010).","journal-title":"Ther. Adv. Musculoskelet Dis."},{"key":"BFsrep36401_CR35","doi-asserted-by":"publisher","first-page":"2747","DOI":"10.1002\/art.24781","volume":"60","author":"R Axmann","year":"2009","unstructured":"Axmann, R. et al. Inhibition of interleukin-6 receptor directly blocks osteoclast formation in vitro and in vivo. Arthritis Rheum. 60, 2747\u20132756 (2009).","journal-title":"Arthritis Rheum."},{"key":"BFsrep36401_CR36","first-page":"1595","volume":"25","author":"YE Henrotin","year":"1998","unstructured":"Henrotin, Y. E. et al. Nitric oxide downregulates interleukin 1beta (IL-1beta) stimulated IL-6, IL-8, and prostaglandin E2 production by human chondrocytes. J. Rheumatol. 25, 1595\u20131601 (1998).","journal-title":"J. Rheumatol."},{"key":"BFsrep36401_CR37","doi-asserted-by":"publisher","first-page":"3873","DOI":"10.4049\/jimmunol.166.6.3873","volume":"166","author":"L Connelly","year":"2001","unstructured":"Connelly, L., Palacios-Callender, M., Ameixa, C., Moncada, S. & Hobbs, A. J. Biphasic regulation of NF-kappa B activity underlies the pro- and anti-inflammatory actions of nitric oxide. J. Immunol. 166, 3873\u20133881 (2001).","journal-title":"J. Immunol."},{"key":"BFsrep36401_CR38","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1016\/j.niox.2008.07.005","volume":"19","author":"SC Rosa","year":"2008","unstructured":"Rosa, S. C., Judas, F., Lopes, M. C. & Mendes, A. F. Nitric oxide synthase isoforms and NF-kappaB activity in normal and osteoarthritic human chondrocytes: regulation by inducible nitric oxide. Nitric Oxide. 19, 276\u2013283 (2008).","journal-title":"Nitric Oxide."},{"key":"BFsrep36401_CR39","first-page":"0","volume":"13","author":"K Zaheer","year":"2015","unstructured":"Zaheer, K. & Akhtar, M. H. An Updated Review of Dietary Isoflavones: Nutrition, Processing, Bioavailability and Impacts on Human Health. Crit. Rev. Food Sci. Nutr. 13, 0 (2015).","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"BFsrep36401_CR40","doi-asserted-by":"publisher","first-page":"2407","DOI":"10.1002\/mnfr.201500240","volume":"59","author":"A Kurrat","year":"2015","unstructured":"Kurrat, A. et al. Lifelong exposure to dietary isoflavones reduces risk of obesity in ovariectomized Wistar rats. Mol. Nutr. Food Res. 59, 2407\u20132418 (2015).","journal-title":"Mol. Nutr. Food Res."},{"key":"BFsrep36401_CR41","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1515\/znc-1976-9-1005","volume":"31","author":"JL Ingham","year":"1976","unstructured":"Ingham, J. L. Induced Isoflavonoids from Fungus-Infected Stems of Pigeon Pea (Cajanus-Cajan). Z. Naturforsch. C. 31, 504\u2013508 (1976).","journal-title":"Z. Naturforsch. C."},{"key":"BFsrep36401_CR42","doi-asserted-by":"publisher","first-page":"7502","DOI":"10.1021\/jf071296w","volume":"55","author":"O Cuesta-Rubio","year":"2007","unstructured":"Cuesta-Rubio, O. et al. Chemical characterization of Cuban propolis by HPLC-PDA, HPLC-MS, and NMR: the Brown, Red, and Yellow Cuban varieties of propolis. J. Agr. Food Chem. 55, 7502\u20137509 (2007).","journal-title":"J. Agr. Food Chem."},{"key":"BFsrep36401_CR43","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1161\/01.RES.25.3.315","volume":"25","author":"S Baez","year":"1969","unstructured":"Baez, S. Simultaneous measurements of radii and wall thickness of microvessels in the anesthetized rat. Circ. Res. 25, 315\u2013329 (1969).","journal-title":"Circ. Res."},{"key":"BFsrep36401_CR44","doi-asserted-by":"publisher","first-page":"1267","DOI":"10.2337\/diab.40.10.1267","volume":"40","author":"ZB Fortes","year":"1991","unstructured":"Fortes, Z. B., Farsky, S. P., Oliveira, M. A. & Garcia-Leme, J. Direct vital microscopic study of defective leukocyte-endothelial interaction in diabetes mellitus. Diabetes. 40, 1267\u20131273 (1991).","journal-title":"Diabetes."},{"key":"BFsrep36401_CR45","doi-asserted-by":"publisher","first-page":"609","DOI":"10.4049\/jimmunol.180.1.609","volume":"180","author":"MH Napimoga","year":"2008","unstructured":"Napimoga, M. H. et al. Peroxisome proliferator-activated receptor-gamma ligand, 15-deoxy-Delta12,14-prostaglandin J2, reduces neutrophil migration via a nitric oxide pathway. J. Immunol. 180, 609\u2013617 (2008).","journal-title":"J. Immunol."},{"key":"BFsrep36401_CR46","doi-asserted-by":"publisher","first-page":"1755","DOI":"10.1073\/pnas.0409225102","volume":"102","author":"TM Cunha","year":"2005","unstructured":"Cunha, T. M. et al. A cascade of cytokines mediates mechanical inflammatory hypernociception in mice. Proc. Natl. Acad. Sci. USA 102, 1755\u20131760 (2005).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFsrep36401_CR47","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/0003-2697(82)90118-X","volume":"126","author":"LC Green","year":"1982","unstructured":"Green, L. C. et al. Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal. Biochem. 126, 131\u2013138 (1982).","journal-title":"Anal. Biochem."},{"key":"BFsrep36401_CR48","doi-asserted-by":"publisher","first-page":"604","DOI":"10.1111\/bph.12103","volume":"169","author":"TY Park","year":"2013","unstructured":"Park, T. Y., Baik, E. J. & Lee, S. H. Prostaglandin E(2)-induced intercellular adhesion molecule-1 expression is mediated by cAMP\/Epac signalling modules in bEnd.3 brain endothelial cells. Br. J. Pharmacol. 169, 604\u2013618 (2013).","journal-title":"Br. J. Pharmacol."},{"key":"BFsrep36401_CR49","doi-asserted-by":"publisher","first-page":"604","DOI":"10.1189\/jlb.0703340","volume":"75","author":"R Boxio","year":"2004","unstructured":"Boxio, R., Bossenmeyer-Pourie, C., Steinckwich, N., Dournon, C. & Nusse, O. Mouse bone marrow contains large numbers of functionally competent neutrophils. J. Leukoc. Biol. 75, 604\u2013611 (2004).","journal-title":"J. Leukoc. Biol."},{"key":"BFsrep36401_CR50","doi-asserted-by":"publisher","first-page":"946","DOI":"10.1124\/jpet.300.3.946","volume":"300","author":"JE Da Silva-Santos","year":"2002","unstructured":"Da Silva-Santos, J. E., Santos-Silva, M. C., Cunha, F. Q. & Assreuy, J. The role of ATP-sensitive potassium channels in neutrophil migration and plasma exudation. J. Pharmacol. Exp. Ther. 300, 946\u2013951 (2002).","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"BFsrep36401_CR51","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/0022-1759(95)00072-I","volume":"184","author":"I Vermes","year":"1995","unstructured":"Vermes, I., Haanen, C., Steffens-Nakken, H. & Reutelingsperger, C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J. Immunol. Methods. 184, 39\u201351 (1995).","journal-title":"Immunol. Methods."},{"key":"BFsrep36401_CR52","doi-asserted-by":"publisher","first-page":"1269","DOI":"10.1038\/nprot.2007.173","volume":"2","author":"DD Brand","year":"2007","unstructured":"Brand, D. D., Latham, K. A. & Rosloniec, E. F. Collagen-induced arthritis. Nat. Protoc. 2, 1269\u20131275 (2007).","journal-title":"Nat. Protoc."}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep36401.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep36401","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep36401.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T01:52:56Z","timestamp":1672883576000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep36401"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,7]]},"references-count":52,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,12,21]]}},"alternative-id":["BFsrep36401"],"URL":"https:\/\/doi.org\/10.1038\/srep36401","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,7]]},"assertion":[{"value":"19 February 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 October 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 November 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"36401"}}