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Although Slc22a14 is a member of the organic anion\/cation transporter family, its expression profile and physiological role have not been elucidated. Here, we show that Slc22a14 is crucial for sperm motility and male fertility in mice. Slc22a14 is expressed specifically in male germ cells, and mice lacking the<jats:italic>Slc22a14<\/jats:italic>gene show severe male infertility. Although the overall differentiation of sperm was normal,<jats:italic>Slc22a14<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>cauda epididymal spermatozoa showed reduced motility with abnormal flagellar bending. Further, the ability to migrate into the female reproductive tract and fertilise the oocyte were also impaired in<jats:italic>Slc22a14<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>spermatozoa. The abnormal flagellar bending was thought to be partly caused by osmotic cell swelling since osmotic challenge or membrane permeabilisation treatment alleviated the tail abnormality. In addition, we found structural abnormalities in<jats:italic>Slc22a14<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>sperm cells: the annulus, a ring-like structure at the mid-piece\u2013principal piece junction, was disorganised, and expression and localisation of septin 4, an annulus component protein that is essential for the annulus formation, was also impaired. Taken together, our results demonstrated that Slc22a14 plays a pivotal role in normal flagellar structure, motility and fertility in mouse spermatozoa.<\/jats:p>","DOI":"10.1038\/srep36468","type":"journal-article","created":{"date-parts":[[2016,11,4]],"date-time":"2016-11-04T09:32:22Z","timestamp":1478251942000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["A critical role of solute carrier 22a14 in sperm motility and male fertility in mice"],"prefix":"10.1038","volume":"6","author":[{"given":"Shin-ya","family":"Maruyama","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Momoe","family":"Ito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuusuke","family":"Ikami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Okitsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chizuru","family":"Ito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyotaka","family":"Toshimori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wataru","family":"Fujii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiichiro","family":"Yogo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,11,4]]},"reference":[{"key":"BFsrep36468_CR1","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1007\/s00424-003-1192-y","volume":"447","author":"MA Hediger","year":"2004","unstructured":"Hediger, M. 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