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Currently, <jats:italic>S. suis<\/jats:italic>\u2013platelet interactions are poorly understood. Here, we found that suilysin (SLY), the <jats:italic>S. suis<\/jats:italic> cholesterol-dependent cytolysin (CDC), was the sole stimulus of <jats:italic>S. suis<\/jats:italic> that induced platelet-neutrophil complexes (PNC) formation. Furthermore, P-selectin released in \u03b1-granules mediated PNC formation. This process was triggered by the SLY-induced pore forming-dependent Ca<jats:sup>2+<\/jats:sup> influx. Moreover, we demonstrated that the Ca<jats:sup>2+<\/jats:sup> influx triggered an MLCK-dependent pathway playing critical roles in P-selectin activation and PNC formation, however, PLC-\u03b2-IP3\/DAG-MLCK and Rho-ROCK-MLCK signalling were not involved. Additionally, the \u201coutside-in\u201d signalling had a smaller effect on the SLY-induced P-selectin release and PNC formation. Interestingly, other CDCs including pneumolysin and streptolysin O have also been found to induce PNC formation in a pore forming-dependent Ca<jats:sup>2+<\/jats:sup> influx manner. It is possible that the bacterial CDC-mediated PNC formation is a similar response mechanism used by a wide range of bacteria. These findings may provide useful insight for discovering potential therapeutic targets for <jats:italic>S. suis<\/jats:italic>-associated STSS.<\/jats:p>","DOI":"10.1038\/srep36787","type":"journal-article","created":{"date-parts":[[2016,11,10]],"date-time":"2016-11-10T11:02:38Z","timestamp":1478775758000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Suilysin-induced Platelet-Neutrophil Complexes Formation is Triggered by Pore Formation-dependent Calcium Influx"],"prefix":"10.1038","volume":"6","author":[{"given":"Shengwei","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuling","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shaolong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shujing","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keke","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingyu","family":"Lv","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongqiang","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,11,10]]},"reference":[{"key":"BFsrep36787_CR1","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1086\/596763","volume":"48","author":"HFL Wertheim","year":"2009","unstructured":"Wertheim, H. 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