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However, the interaction between IGF-1\/IGF-1R and<jats:italic>hsa-let-7c<\/jats:italic>on the committed differentiation of stem cells from apical papilla (SCAPs) remains unclear. In this study, human SCAPs were isolated and treated with IGF-1 and<jats:italic>hsa-let-7c<\/jats:italic>over\/low-expression viruses. The odonto\/osteogenic differentiation of these stem cells and the involvement of mitogen-activated protein kinase (MAPK) pathway were subsequently investigated. Alizarin red staining showed that<jats:italic>hsa-let-7c<\/jats:italic>low-expression can significantly promote the mineralization of IGF-1 treated SCAPs, while<jats:italic>hsa-let-7c<\/jats:italic>over-expression can decrease the calcium deposition of IGF-1 treated SCAPs. Western blot assay and real-time reverse transcription polymerase chain reaction further demonstrated that the expression of odonto\/osteogenic markers (ALP, RUNX2\/<jats:italic>RUNX2<\/jats:italic>, OSX\/<jats:italic>OSX<\/jats:italic>, OCN\/<jats:italic>OCN<\/jats:italic>, COL-I\/<jats:italic>COL-I<\/jats:italic>, DSPP\/<jats:italic>DSP<\/jats:italic>, and DMP-1\/<jats:italic>DMP-1<\/jats:italic>) in IGF-1 treated SCAPs were significantly upregulated in<jats:italic>Let-7c<\/jats:italic>-low group. On the contrary,<jats:italic>hsa-let-7c<\/jats:italic>over-expression could downregulate the expression of these odonto\/osteogenic markers. Moreover, western blot assay showed that the JNK and p38 MAPK signaling pathways were activated in<jats:italic>Let-7c<\/jats:italic>-low SCAPs but inhibited in<jats:italic>Let-7c<\/jats:italic>-over SCAPs. Together, the IGF-1\/IGF-1R\/<jats:italic>hsa-let-7c<\/jats:italic>axis can control the odonto\/osteogenic differentiation of IGF-1-treated SCAPs via the regulation of JNK and p38 MAPK signaling pathways.<\/jats:p>","DOI":"10.1038\/srep36922","type":"journal-article","created":{"date-parts":[[2016,11,11]],"date-time":"2016-11-11T10:15:57Z","timestamp":1478859357000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["IGF-1\/IGF-1R\/hsa-let-7c axis regulates the committed differentiation of stem cells from apical papilla"],"prefix":"10.1038","volume":"6","author":[{"given":"Shu","family":"Ma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Genxia","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Jin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiyao","family":"Pang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanqiu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zilu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinhua","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,11,11]]},"reference":[{"key":"BFsrep36922_CR1","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1590\/S0103-64402011000200001","volume":"22","author":"C Estrela","year":"2011","unstructured":"Estrela, C., Alencar, A. 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