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Total extractable organic P (P<jats:sub>o<\/jats:sub>) concentrations ranged from 504 to 1643\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup> and 2318 to 8395\u2009mg\u2009kg<jats:sup>\u22121<\/jats:sup> for aquatic macrophytes and algae, respectively. Using <jats:sup>31<\/jats:sup>P NMR spectroscopy, 11 P<jats:sub>o<\/jats:sub> species were detected in the mono- and diester region. Additionally, orthophosphate, pyrophosphate and phosphonates were also detected. Using EH, phytate-like P was identified as the prevalent class of enzyme-labile P<jats:sub>o<\/jats:sub>, followed by labile monoester- and diester-P. Comparison of the NMR and EH data indicated that the distribution pattern of major P forms in the samples determined by the two methods was similar (<jats:italic>r<\/jats:italic>\u2009=\u20090.712, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Additional <jats:sup>31<\/jats:sup>P NMR spectroscopic analysis of extracts following EH showed significant decreases in the monoester and pyrophosphate regions, with a corresponding increase in the orthophosphate signal, as compared to unhydrolyzed extracts. Based on these quantity and hydrolysis data, we proposed that recycling of P<jats:sub>o<\/jats:sub> in vegetative biomass residues is an important mechanism for long-term self-regulation of available P for algal blooming in eutrophic lakes.<\/jats:p>","DOI":"10.1038\/srep37164","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T10:29:45Z","timestamp":1479292185000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Forms and Lability of Phosphorus in Algae and Aquatic Macrophytes Characterized by Solution 31P NMR Coupled with Enzymatic Hydrolysis"],"prefix":"10.1038","volume":"6","author":[{"given":"Weiying","family":"Feng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanrong","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengchang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongqi","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P.","family":"Giesy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,11,16]]},"reference":[{"key":"BFsrep37164_CR1","doi-asserted-by":"publisher","first-page":"366","DOI":"10.1016\/j.envpol.2007.06.024","volume":"152","author":"R Zhang","year":"2008","unstructured":"Zhang, R. et al. 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