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In particular, while ankyrin (Ank-1) mutations account for approximately 50% of hereditary spherocytosis (HS) cases, an association with malaria is not well-established, and conflicting evidence has been reported. We describe a novel N-ethyl-N-nitrosourea (ENU)-induced ankyrin mutation MRI61689 that gives rise to two different ankyrin transcripts: one with an introduced splice acceptor site resulting a frameshift, the other with a skipped exon.\n                    <jats:italic>Ank-1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>(MRI61689\/<\/jats:italic>\n                      +)\n                    <\/jats:sup>\n                    mice exhibit an HS-like phenotype including reduction in mean corpuscular volume (MCV), increased osmotic fragility and reduced RBC deformability. They were also found to be resistant to rodent malaria\n                    <jats:italic>Plasmodium chabaudi<\/jats:italic>\n                    infection. Parasites in\n                    <jats:italic>Ank-1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>(MRI61689\/<\/jats:italic>\n                      +)\n                    <\/jats:sup>\n                    erythrocytes grew normally, but red cells showed resistance to merozoite invasion. Uninfected\n                    <jats:italic>Ank-1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>(MRI61689\/<\/jats:italic>\n                      +)\n                    <\/jats:sup>\n                    erythrocytes were also more likely to be cleared from circulation during infection; the \u201cbystander effect\u201d. This increased clearance is a novel resistance mechanism which was not observed in previous ankyrin mouse models. We propose that this bystander effect is due to reduced deformability of\n                    <jats:italic>Ank-1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>(MRI61689\/<\/jats:italic>\n                      +)\n                    <\/jats:sup>\n                    erythrocytes. This paper highlights the complex roles ankyrin plays in mediating malaria resistance.\n                  <\/jats:p>","DOI":"10.1038\/srep37197","type":"journal-article","created":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T06:31:07Z","timestamp":1479277867000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A novel ENU-induced ankyrin-1 mutation impairs parasite invasion and increases erythrocyte clearance during malaria infection in mice"],"prefix":"10.1038","volume":"6","author":[{"given":"Hong Ming","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis C.","family":"Bauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick M.","family":"Lelliott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Greth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendan J.","family":"McMorran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon J.","family":"Foote","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaetan","family":"Burgio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,11,16]]},"reference":[{"key":"BFsrep37197_CR1","unstructured":"W.H.O. 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