{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T18:22:30Z","timestamp":1770834150177,"version":"3.50.1"},"reference-count":51,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,11,23]],"date-time":"2016-11-23T00:00:00Z","timestamp":1479859200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,11,23]],"date-time":"2016-11-23T00:00:00Z","timestamp":1479859200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Oxidative stress induces the activation of liver fibrogenic cells (myofibroblasts), thus promoting the expression of fibrosis-related genes, leading to hepatic fibrogenesis. MicroRNAs (miRNAs) are a new class of small RNAs ~18\u201325 nucleotides in length involved in post-transcriptional regulation of gene expression. Wound-healing and remodeling processes in liver fibrosis have been associated with changes in hepatic miRNA expression. However, the role of miR-706 in liver fibrogenesis is currently unknown. In the present study, we show that miR-706 is abundantly expressed in hepatocytes. Moreover, oxidative stress leads to a significant downregulation of miR-706, and the further reintroduction of miR-706 inhibits oxidative stress-induced expression of fibrosis-related markers such as \u03b1-SMA. Subsequent studies revealed that miR-706 directly inhibits PKC\u03b1 and TAOK1 expression <jats:italic>via<\/jats:italic> binding to the 3\u2032-untranslated region, preventing epithelial mesenchymal transition. <jats:italic>In vivo<\/jats:italic> studies showed that intravenous injection of miR-706 agomir successfully increases hepatic miR-706 and decreases \u03b1-SMA, PKC\u03b1, and TAOK1 protein levels in livers of carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>)-treated mice. In summary, this study reveals a protective role for miR-706 by blocking the oxidative stress-induced activation of PKC\u03b1\/TAOK1. Our results further identify a major implication for miR-706 in preventing hepatic fibrogenesis and suggest that miR-706 may be a suitable molecular target for anti-fibrosis therapy.<\/jats:p>","DOI":"10.1038\/srep37509","type":"journal-article","created":{"date-parts":[[2016,11,23]],"date-time":"2016-11-23T10:29:24Z","timestamp":1479896964000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["miR-706 inhibits the oxidative stress-induced activation of PKC\u03b1\/TAOK1 in liver fibrogenesis"],"prefix":"10.1038","volume":"6","author":[{"given":"Ruili","family":"Yin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duo","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuxian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuying","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,11,23]]},"reference":[{"key":"BFsrep37509_CR1","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1053\/jhep.2003.50304","volume":"38","author":"HD De Las","year":"2003","unstructured":"De Las, H. D. et al. Increased carbon monoxide production in patients with cirrhosis with and without spontaneous bacterial peritonitis. Hepatology 38, 452\u2013459 (2003).","journal-title":"Hepatology"},{"key":"BFsrep37509_CR2","doi-asserted-by":"publisher","first-page":"S38","DOI":"10.1016\/S0168-8278(02)00429-4","volume":"38","author":"SL Friedman","year":"2003","unstructured":"Friedman, S. L. Liver fibrosis \u2013 from bench to bedside. J Hepatol. 38 Suppl 1, S38\u2013S53 (2003).","journal-title":"J Hepatol."},{"key":"BFsrep37509_CR3","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1159\/000317248","volume":"16","author":"WA Zatonski","year":"2010","unstructured":"Zatonski, W. A. et al. Liver cirrhosis mortality in Europe, with special attention to Central and Eastern Europe. Eur Addict Res. 16, 193\u2013201 (2010).","journal-title":"Eur Addict Res."},{"key":"BFsrep37509_CR4","doi-asserted-by":"publisher","first-page":"209","DOI":"10.3389\/fphys.2015.00209","volume":"6","author":"J Lambrecht","year":"2015","unstructured":"Lambrecht, J., Mannaerts, I. & van Grunsven, L. A. The role of miRNAs in stress-responsive hepatic stellate cells during liver fibrosis. Front Physiol. 6, 209 (2015).","journal-title":"Front Physiol."},{"key":"BFsrep37509_CR5","doi-asserted-by":"publisher","first-page":"S108","DOI":"10.1111\/j.1440-1746.2007.05295.x","volume":"23","author":"DA Mann","year":"2008","unstructured":"Mann, D. A. & Mann, J. Epigenetic regulation of hepatic stellate cell activation. J Gastroenterol Hepatol. 23 Suppl 1, S108\u2013S111 (2008).","journal-title":"J Gastroenterol Hepatol."},{"key":"BFsrep37509_CR6","doi-asserted-by":"publisher","first-page":"E3297","DOI":"10.1073\/pnas.1400062111","volume":"111","author":"K Iwaisako","year":"2014","unstructured":"Iwaisako, K. et al. Origin of myofibroblasts in the fibrotic liver in mice. Proc Natl Acad Sci USA 111, E3297\u2013E3305 (2014).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep37509_CR7","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/j.ccr.2011.01.020","volume":"19","author":"M Quante","year":"2011","unstructured":"Quante, M. et al. Bone marrow-derived myofibroblasts contribute to the mesenchymal stem cell niche and promote tumor growth. Cancer Cell 19, 257\u2013272 (2011).","journal-title":"Cancer Cell"},{"key":"BFsrep37509_CR8","doi-asserted-by":"publisher","first-page":"23337","DOI":"10.1074\/jbc.M700194200","volume":"282","author":"M Zeisberg","year":"2007","unstructured":"Zeisberg, M. et al. Fibroblasts derive from hepatocytes in liver fibrosis via epithelial to mesenchymal transition. J Biol Chem 282, 23337\u201323347 (2007).","journal-title":"J Biol Chem"},{"key":"BFsrep37509_CR9","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1053\/j.gastro.2008.04.038","volume":"135","author":"S Dooley","year":"2008","unstructured":"Dooley, S. et al. Hepatocyte-specific Smad7 expression attenuates TGF-beta-mediated fibrogenesis and protects against liver damage. GASTROENTEROLOGY 135, 642\u2013659 (2008).","journal-title":"GASTROENTEROLOGY"},{"key":"BFsrep37509_CR10","doi-asserted-by":"publisher","first-page":"909","DOI":"10.1002\/hep.22397","volume":"48","author":"T Nitta","year":"2008","unstructured":"Nitta, T., Kim, J. S., Mohuczy, D. & Behrns, K. E. Murine cirrhosis induces hepatocyte epithelial mesenchymal transition and alterations in survival signaling pathways. Hepatology 48, 909\u2013919 (2008).","journal-title":"Hepatology"},{"key":"BFsrep37509_CR11","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.biopha.2014.10.028","volume":"69","author":"Z Liu","year":"2015","unstructured":"Liu, Z., Wang, J., Guo, C. & Fan, X. microRNA-21 mediates epithelial-mesenchymal transition of human hepatocytes via PTEN\/Akt pathway. Biomed Pharmacother 69, 24\u201328 (2015).","journal-title":"Biomed Pharmacother"},{"key":"BFsrep37509_CR12","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1002\/path.4373","volume":"234","author":"X Tu","year":"2014","unstructured":"Tu, X. et al. MicroRNA-101 suppresses liver fibrosis by targeting the TGFbeta signalling pathway. J Pathol 234, 46\u201359 (2014).","journal-title":"J Pathol"},{"key":"BFsrep37509_CR13","doi-asserted-by":"publisher","first-page":"2706","DOI":"10.1002\/jcp.24995","volume":"230","author":"S Zhao","year":"2015","unstructured":"Zhao, S. et al. Loss of MicroRNA-101 Promotes Epithelial to Mesenchymal Transition in Hepatocytes. J Cell Physiol 230, 2706\u20132717 (2015).","journal-title":"J Cell Physiol"},{"key":"BFsrep37509_CR14","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/j.cell.2009.01.002","volume":"136","author":"DP Bartel","year":"2009","unstructured":"Bartel, D. P. MicroRNAs: target recognition and regulatory functions. Cell 136, 215\u2013233 (2009).","journal-title":"Cell"},{"key":"BFsrep37509_CR15","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1038\/nrg3162","volume":"13","author":"AE Pasquinelli","year":"2012","unstructured":"Pasquinelli, A. E. MicroRNAs and their targets: recognition, regulation and an emerging reciprocal relationship. Nat Rev Genet 13, 271\u2013282 (2012).","journal-title":"Nat Rev Genet"},{"key":"BFsrep37509_CR16","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1016\/j.cell.2012.04.005","volume":"149","author":"MS Ebert","year":"2012","unstructured":"Ebert, M. S. & Sharp, P. A. Roles for microRNAs in conferring robustness to biological processes. Cell 149, 515\u2013524 (2012).","journal-title":"Cell"},{"key":"BFsrep37509_CR17","doi-asserted-by":"crossref","unstructured":"Wu, H., Ng, R., Chen, X., Steer, C. J. & Song, G. MicroRNA-21 is a potential link between non-alcoholic fatty liver disease and hepatocellular carcinoma via modulation of the HBP1-p53-Srebp1c pathway. Gut (2015).","DOI":"10.1136\/gutjnl-2014-308430"},{"key":"BFsrep37509_CR18","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/j.chom.2014.07.006","volume":"16","author":"CD Sedano","year":"2014","unstructured":"Sedano, C. D. & Sarnow, P. Hepatitis C virus subverts liver-specific miR-122 to protect the viral genome from exoribonuclease Xrn2. Cell Host Microbe 16, 257\u2013264 (2014).","journal-title":"Cell Host Microbe"},{"key":"BFsrep37509_CR19","doi-asserted-by":"publisher","first-page":"5885","DOI":"10.1007\/s13277-015-4456-1","volume":"37","author":"WF Li","year":"2016","unstructured":"Li, W. F., Dai, H., Ou, Q., Zuo, G. Q. & Liu, C. A. Overexpression of microRNA-30a-5p inhibits liver cancer cell proliferation and induces apoptosis by targeting MTDH\/PTEN\/AKT pathway. Tumour Biol 37, 5885\u20135895 (2016).","journal-title":"Tumour Biol"},{"key":"BFsrep37509_CR20","doi-asserted-by":"publisher","first-page":"844","DOI":"10.1177\/0192623315573587","volume":"43","author":"T Izawa","year":"2015","unstructured":"Izawa, T., Horiuchi, T., Atarashi, M., Kuwamura, M. & Yamate, J. Anti-fibrotic Role of miR-214 in Thioacetamide-induced Liver Cirrhosis in Rats. Toxicol Pathol 43, 844\u2013851 (2015).","journal-title":"Toxicol Pathol"},{"key":"BFsrep37509_CR21","doi-asserted-by":"publisher","first-page":"1522","DOI":"10.1111\/j.1742-4658.2011.08075.x","volume":"278","author":"WQ Li","year":"2011","unstructured":"Li, W. Q. et al. The rno-miR-34 family is upregulated and targets ACSL1 in Dimethylnitrosamine-induced hepatic fibrosis in rats. FEBS J. 278, 1522\u20131532 (2011).","journal-title":"FEBS J."},{"key":"BFsrep37509_CR22","doi-asserted-by":"crossref","first-page":"12224","DOI":"10.18632\/oncotarget.3652","volume":"6","author":"C Zeng","year":"2015","unstructured":"Zeng, C. et al. Identification of a novel TGF-beta-miR-122-fibronectin 1\/serum response factor signaling cascade and its implication in hepatic fibrogenesis. Oncotarget 6, 12224\u201312233 (2015).","journal-title":"Oncotarget"},{"key":"BFsrep37509_CR23","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1002\/hep.23922","volume":"53","author":"C Roderburg","year":"2011","unstructured":"Roderburg, C. et al. Micro-RNA profiling reveals a role for miR-29 in human and murine liver fibrosis. Hepatology 53, 209\u2013218 (2011).","journal-title":"Hepatology"},{"key":"BFsrep37509_CR24","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1039\/C0MB00230E","volume":"7","author":"ZY Li","year":"2011","unstructured":"Li, Z. Y., Na, H. M., Peng, G., Pu, J. & Liu, P. Alteration of microRNA expression correlates to fatty acid-mediated insulin resistance in mouse myoblasts. Mol Biosyst 7, 871\u2013877 (2011).","journal-title":"Mol Biosyst"},{"key":"BFsrep37509_CR25","doi-asserted-by":"publisher","first-page":"1643","DOI":"10.1007\/s00705-010-0749-2","volume":"155","author":"H Lian","year":"2010","unstructured":"Lian, H. et al. A laboratory-attenuated vesicular stomatitis virus induces apoptosis and alters the cellular microRNA expression profile in BHK cells. Arch Virol 155, 1643\u20131653 (2010).","journal-title":"Arch Virol"},{"key":"BFsrep37509_CR26","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1016\/j.cellsig.2003.09.002","volume":"16","author":"W Lang","year":"2004","unstructured":"Lang, W., Wang, H., Ding, L. & Xiao, L. Cooperation between PKC-alpha and PKC-epsilon in the regulation of JNK activation in human lung cancer cells. Cell Signal 16, 457\u2013467 (2004).","journal-title":"Cell Signal"},{"key":"BFsrep37509_CR27","doi-asserted-by":"publisher","first-page":"1820","DOI":"10.1016\/j.cellsig.2007.05.014","volume":"19","author":"ME Barnett","year":"2007","unstructured":"Barnett, M. E., Madgwick, D. K. & Takemoto, D. J. Protein kinase C as a stress sensor. Cell Signal 19, 1820\u20131829 (2007).","journal-title":"Cell Signal"},{"key":"BFsrep37509_CR28","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.mam.2007.09.001","volume":"29","author":"M Nitti","year":"2008","unstructured":"Nitti, M., Pronzato, M. A., Marinari, U. M. & Domenicotti, C. PKC signaling in oxidative hepatic damage. Mol Aspects Med. 29, 36\u201342 (2008).","journal-title":"Mol Aspects Med."},{"key":"BFsrep37509_CR29","doi-asserted-by":"publisher","first-page":"28625","DOI":"10.1074\/jbc.273.44.28625","volume":"273","author":"M Hutchison","year":"1998","unstructured":"Hutchison, M., Berman, K. S. & Cobb, M. H. Isolation of TAO1, a protein kinase that activates MEKs in stress-activated protein kinase cascades. J Biol Chem. 273, 28625\u201328632 (1998).","journal-title":"J Biol Chem."},{"key":"BFsrep37509_CR30","doi-asserted-by":"publisher","first-page":"2005","DOI":"10.1038\/sj.emboj.7601668","volume":"26","author":"M Raman","year":"2007","unstructured":"Raman, M., Earnest, S., Zhang, K., Zhao, Y. & Cobb, M. H. TAO kinases mediate activation of p38 in response to DNA damage. EMBO J 26, 2005\u20132014 (2007).","journal-title":"EMBO J"},{"key":"BFsrep37509_CR31","doi-asserted-by":"publisher","first-page":"6129","DOI":"10.1038\/sj.onc.1206605","volume":"22","author":"JT Yustein","year":"2003","unstructured":"Yustein, J. T. et al. Comparative studies of a new subfamily of human Ste20-like kinases: homodimerization, subcellular localization, and selective activation of MKK3 and p38. Oncogene 22, 6129\u20136141 (2003).","journal-title":"Oncogene"},{"key":"BFsrep37509_CR32","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1186\/2045-3701-2-34","volume":"2","author":"R Kostadinova","year":"2012","unstructured":"Kostadinova, R. et al. GW501516-activated PPARbeta\/delta promotes liver fibrosis via p38-JNK MAPK-induced hepatic stellate cell proliferation. Cell Biosci. 2, 34 (2012).","journal-title":"Cell Biosci."},{"key":"BFsrep37509_CR33","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1055\/s-2008-1040457","volume":"10","author":"H Tsukamoto","year":"1990","unstructured":"Tsukamoto, H., Matsuoka, M. & French, S. W. Experimental models of hepatic fibrosis: a review. Semin Liver Dis. 10, 56\u201365 (1990).","journal-title":"Semin Liver Dis."},{"key":"BFsrep37509_CR34","doi-asserted-by":"publisher","first-page":"G126","DOI":"10.1152\/ajpgi.00299.2009","volume":"298","author":"JI Yang","year":"2010","unstructured":"Yang, J. I. et al. Synergistic antifibrotic efficacy of statin and protein kinase C inhibitor in hepatic fibrosis. Am J Physiol Gastrointest Liver Physiol. 298, G126\u2013G132 (2010).","journal-title":"Am J Physiol Gastrointest Liver Physiol."},{"key":"BFsrep37509_CR35","doi-asserted-by":"publisher","first-page":"340","DOI":"10.1016\/j.jhep.2009.12.008","volume":"52","author":"P Taura,","year":"2010","unstructured":"Taura, P. et al. The use of beta-adrenergic drugs improves hepatic oxygen metabolism in cirrhotic patients undergoing liver resection. J Hepatol 52, 340\u2013347 (2010).","journal-title":"J Hepatol"},{"key":"BFsrep37509_CR36","doi-asserted-by":"crossref","unstructured":"Li X. et al. MiR-124 protects human hepatic L02 cells from H2O2-induced apoptosis by targeting Rab38 gene. Biochem Biophys Res Commun. 18; 450 (1): 148-53 (2014).","DOI":"10.1016\/j.bbrc.2014.05.085"},{"key":"BFsrep37509_CR37","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1146\/annurev-pathol-011110-130246","volume":"6","author":"V Hernandez-Gea","year":"2011","unstructured":"Hernandez-Gea, V. & Friedman, S. L. Pathogenesis of liver fibrosis. Annu Rev Pathol 6, 425\u2013456 (2011).","journal-title":"Annu Rev Pathol"},{"key":"BFsrep37509_CR38","doi-asserted-by":"publisher","first-page":"1655","DOI":"10.1053\/j.gastro.2008.03.003","volume":"134","author":"SL Friedman","year":"2008","unstructured":"Friedman, S. L. Mechanisms of hepatic fibrogenesis. Gastroenterology 134, 1655\u20131669 (2008).","journal-title":"Gastroenterology"},{"key":"BFsrep37509_CR39","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1159\/000362249","volume":"39","author":"S Sun","year":"2014","unstructured":"Sun, S. et al. Egr-1 mediates chronic hypoxia-induced renal interstitial fibrosis via the PKC\/ERK pathway. Am J Nephrol. 39, 436\u2013448 (2014).","journal-title":"Am J Nephrol."},{"key":"BFsrep37509_CR40","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/j.drup.2008.07.001","volume":"11","author":"M Sabbah","year":"2008","unstructured":"Sabbah, M. et al. Molecular signature and therapeutic perspective of the epithelial-to-mesenchymal transitions in epithelial cancers. Drug Resist Updat 11, 123\u2013151 (2008).","journal-title":"Drug Resist Updat"},{"key":"BFsrep37509_CR41","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1267\/ahc.14030","volume":"47","author":"S Takekoshi","year":"2014","unstructured":"Takekoshi, S., Kitatani, K. & Yamamoto, Y. Roles of Oxidized Diacylglycerol for Carbon Tetrachloride-induced Liver Injury and Fibrosis in Mouse. Acta Histochem Cytochem. 47, 185\u2013194 (2014).","journal-title":"Acta Histochem Cytochem."},{"key":"BFsrep37509_CR42","doi-asserted-by":"publisher","first-page":"12852","DOI":"10.1074\/jbc.M112.410357","volume":"288","author":"Y Wang","year":"2013","unstructured":"Wang, Y. et al. Nuclear factor \u039aB mediates suppression of canonical transient receptor potential 6 expression by reactive oxygen species and protein kinase C in kidney cells. J Biol Chem. 288, 12852\u201312865 (2013).","journal-title":"J Biol Chem."},{"key":"BFsrep37509_CR43","doi-asserted-by":"publisher","first-page":"C304","DOI":"10.1152\/ajpcell.00014.2011","volume":"301","author":"S Graham","year":"2011","unstructured":"Graham, S. et al. Abundance of TRPC6 protein in glomerular mesangial cells is decreased by ROS and PKC in diabetes. Am J Physiol Cell Physiol. 301, C304\u2013C315 (2011).","journal-title":"Am J Physiol Cell Physiol."},{"key":"BFsrep37509_CR44","doi-asserted-by":"publisher","first-page":"3150","DOI":"10.1002\/jnr.21421","volume":"85","author":"Y Quan","year":"2007","unstructured":"Quan, Y., Du, J. & Wang, X. High glucose stimulates GRO secretion from rat microglia via ROS, PKC, and NF-kappaB pathways. J Neurosci Res. 85, 3150\u20133159 (2007).","journal-title":"J Neurosci Res."},{"key":"BFsrep37509_CR45","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1016\/j.biopha.2013.03.014","volume":"67","author":"J Wei","year":"2013","unstructured":"Wei, J., Feng, L., Li, Z., Xu, G. & Fan, X. MicroRNA-21 activates hepatic stellate cells via PTEN\/Akt signaling. Biomed Pharmacother 67, 387\u2013392 (2013).","journal-title":"Biomed Pharmacother"},{"key":"BFsrep37509_CR46","doi-asserted-by":"publisher","first-page":"940","DOI":"10.1016\/j.bbrc.2014.03.037","volume":"446","author":"Y Zhang","year":"2014","unstructured":"Zhang, Y. et al. MiR-29b inhibits collagen maturation in hepatic stellate cells through down-regulating the expression of HSP47 and lysyl oxidase. Biochem Biophys Res Commun. 446, 940\u2013944 (2014).","journal-title":"Biochem Biophys Res Commun."},{"key":"BFsrep37509_CR47","doi-asserted-by":"crossref","first-page":"7325","DOI":"10.18632\/oncotarget.2621","volume":"6","author":"J Wang","year":"2015","unstructured":"Wang, J. et al. microRNA-29b prevents liver fibrosis by attenuating hepatic stellate cell activation and inducing apoptosis through targeting PI3K\/AKT pathway. Oncotarget 6, 7325\u20137338 (2015).","journal-title":"Oncotarget"},{"key":"BFsrep37509_CR48","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1186\/1756-8722-7-42","volume":"7","author":"JJ Wong","year":"2014","unstructured":"Wong, J. J. et al. Identification of nuclear-enriched miRNAs during mouse granulopoiesis. J Hematol Oncol 7, 42 (2014).","journal-title":"J Hematol Oncol"},{"key":"BFsrep37509_CR49","doi-asserted-by":"publisher","first-page":"2448","DOI":"10.1093\/bioinformatics\/btg345","volume":"19","author":"GW Wright","year":"2003","unstructured":"Wright, G. W. & Simon, R. M. A random variance model for detection of differential gene expression in small microarray experiments. Bioinformatics 19, 2448\u20132455 (2003).","journal-title":"Bioinformatics"},{"key":"BFsrep37509_CR50","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1038\/nprot.2015.017","volume":"10","author":"I Mederacke","year":"2015","unstructured":"Mederacke, I., Dapito, D. H., Affo, S., Uchinami, H. & Schwabe, R. F. High-yield and high-purity isolation of hepatic stellate cells from normal and fibrotic mouse livers. Nat Protoc. 10, 305\u2013315 (2015).","journal-title":"Nat Protoc."},{"key":"BFsrep37509_CR51","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1002\/hep.22653","volume":"49","author":"Z Sun","year":"2009","unstructured":"Sun, Z. et al. Recruitment of host progenitor cells in rat liver transplants. Hepatology 49, 587\u2013597 (2009).","journal-title":"Hepatology"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep37509.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep37509","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep37509.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T02:28:27Z","timestamp":1672885707000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep37509"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,23]]},"references-count":51,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,12,23]]}},"alternative-id":["BFsrep37509"],"URL":"https:\/\/doi.org\/10.1038\/srep37509","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,23]]},"assertion":[{"value":"3 August 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 October 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 November 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"37509"}}