{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:38:39Z","timestamp":1790710719001,"version":"4.1.0"},"reference-count":74,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,12,5]],"date-time":"2016-12-05T00:00:00Z","timestamp":1480896000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,12,5]],"date-time":"2016-12-05T00:00:00Z","timestamp":1480896000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cerebral malaria (CM) is a severe complication of <jats:italic>Plasmodium falciparum<\/jats:italic> infection responsible for thousands of deaths in children in sub-Saharan Africa. CM pathogenesis remains incompletely understood but a number of effectors have been proposed, including plasma microparticles (MP). MP numbers are increased in CM patients\u2019 circulation and, in the mouse model, they can be localised within inflamed vessels, suggesting their involvement in vascular damage. In the present work we define, for the first time, the protein cargo of MP during experimental cerebral malaria (ECM) with the overarching hypothesis that this characterisation could help understand CM pathogenesis. Using qualitative and quantitative high-throughput proteomics we compared MP proteins from non-infected and <jats:italic>P<\/jats:italic>. <jats:italic>berghei<\/jats:italic> ANKA-infected mice. More than 360 proteins were identified, 60 of which were differentially abundant, as determined by quantitative comparison using TMT<jats:sup>TM<\/jats:sup> isobaric labelling. Network analyses showed that ECM MP carry proteins implicated in molecular mechanisms relevant to CM pathogenesis, including endothelial activation. Among these proteins, the strict association of carbonic anhydrase I and S100A8 with ECM was verified by western blot on MP from DBA\/1 and C57BL\/6 mice. These results demonstrate that MP protein cargo represents a novel ECM pathogenic trait to consider in the understanding of CM pathogenesis.<\/jats:p>","DOI":"10.1038\/srep37871","type":"journal-article","created":{"date-parts":[[2016,12,5]],"date-time":"2016-12-05T12:46:33Z","timestamp":1480941993000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Exploring experimental cerebral malaria pathogenesis through the characterisation of host-derived plasma microparticle protein content"],"prefix":"10.1038","volume":"6","author":[{"given":"Natalia","family":"Tiberti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharissa L.","family":"Latham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Bush","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert O.","family":"Opoka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandy C.","family":"John","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annette","family":"Juillard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georges E.","family":"Grau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9ry","family":"Combes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,12,5]]},"reference":[{"key":"BFsrep37871_CR1","unstructured":"WHO. World malaria report (2014)."},{"key":"BFsrep37871_CR2","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1203\/00006450-201011001-00524","volume":"68","author":"R Idro","year":"2010","unstructured":"Idro, R., Marsh, K., John, C. C. & Newton, C. R. Cerebral malaria: mechanisms of brain injury and strategies for improved neurocognitive outcome. Pediatr Res 68, 267\u2013274, doi: 10.1203\/00006450-201011001-00524 (2010).","journal-title":"Pediatr Res"},{"key":"BFsrep37871_CR3","doi-asserted-by":"publisher","first-page":"1173","DOI":"10.1016\/S1474-4422(10)70270-2","volume":"9","author":"GL Birbeck","year":"2010","unstructured":"Birbeck, G. L. et al. Blantyre Malaria Project Epilepsy Study (BMPES) of neurological outcomes in retinopathy-positive paediatric cerebral malaria survivors: a prospective cohort study. Lancet Neurol 9, 1173\u20131181, doi: 10.1016\/S1474-4422(10)70270-2 (2010).","journal-title":"Lancet Neurol"},{"key":"BFsrep37871_CR4","doi-asserted-by":"publisher","first-page":"100","DOI":"10.3389\/fcimb.2014.00100","volume":"4","author":"J Storm","year":"2014","unstructured":"Storm, J. & Craig, A. G. Pathogenesis of cerebral malaria\u2013inflammation and cytoadherence. Front Cell Infect Microbiol 4, 100, doi: 10.3389\/fcimb.2014.00100 (2014).","journal-title":"Front Cell Infect Microbiol"},{"key":"BFsrep37871_CR5","doi-asserted-by":"publisher","first-page":"3337","DOI":"10.1002\/art.21350","volume":"52","author":"JH Distler","year":"2005","unstructured":"Distler, J. H. et al. Microparticles as regulators of inflammation: novel players of cellular crosstalk in the rheumatic diseases. Arthritis Rheum 52, 3337\u20133348, doi: 10.1002\/art.21350 (2005).","journal-title":"Arthritis Rheum"},{"key":"BFsrep37871_CR6","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1042\/CS20120309","volume":"124","author":"D Burger","year":"2013","unstructured":"Burger, D. et al. Microparticles: biomarkers and beyond. Clin Sci (Lond) 124, 423\u2013441, doi: 10.1042\/CS20120309 (2013).","journal-title":"Clin Sci (Lond)"},{"key":"BFsrep37871_CR7","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1161\/CIRCRESAHA.113.300858","volume":"114","author":"X Loyer","year":"2014","unstructured":"Loyer, X., Vion, A. C., Tedgui, A. & Boulanger, C. M. Microvesicles as cell-cell messengers in cardiovascular diseases. Circ Res 114, 345\u2013353, doi: 10.1161\/CIRCRESAHA.113.300858 (2014).","journal-title":"Circ Res"},{"key":"BFsrep37871_CR8","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.semcdb.2015.03.009","volume":"40","author":"J Gong","year":"2015","unstructured":"Gong, J., Jaiswal, R., Dalla, P., Luk, F. & Bebawy, M. Microparticles in cancer: A review of recent developments and the potential for clinical application. Semin Cell Dev Biol 40, 35\u201340, doi: 10.1016\/j.semcdb.2015.03.009 (2015).","journal-title":"Semin Cell Dev Biol"},{"key":"BFsrep37871_CR9","doi-asserted-by":"publisher","first-page":"756327","DOI":"10.1155\/2014\/756327","volume":"2014","author":"SM Schindler","year":"2014","unstructured":"Schindler, S. M., Little, J. P. & Klegeris, A. Microparticles: a new perspective in central nervous system disorders. Biomed Res Int 2014, 756327, doi: 10.1155\/2014\/756327 (2014).","journal-title":"Biomed Res Int"},{"key":"BFsrep37871_CR10","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1186\/1471-2121-14-23","volume":"14","author":"NS Barteneva","year":"2013","unstructured":"Barteneva, N. S. et al. Circulating microparticles: square the circle. BMC Cell Biol 14, 23, doi: 10.1186\/1471-2121-14-23 (2013).","journal-title":"BMC Cell Biol"},{"key":"BFsrep37871_CR11","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1002\/mas.21457","volume":"35","author":"G Pocsfalvi","year":"2016","unstructured":"Pocsfalvi, G. et al. Mass spectrometry of extracellular vesicles. Mass Spectrom Rev 35, 3\u201321, doi: 10.1002\/mas.21457 (2016).","journal-title":"Mass Spectrom Rev"},{"key":"BFsrep37871_CR12","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1002\/pmic.201300389","volume":"14","author":"T Katsuda","year":"2014","unstructured":"Katsuda, T., Kosaka, N. & Ochiya, T. The roles of extracellular vesicles in cancer biology: toward the development of novel cancer biomarkers. Proteomics 14, 412\u2013425, doi: 10.1002\/pmic.201300389 (2014).","journal-title":"Proteomics"},{"key":"BFsrep37871_CR13","doi-asserted-by":"publisher","first-page":"e13415","DOI":"10.1371\/journal.pone.0013415","volume":"5","author":"JB Pankoui Mfonkeu","year":"2010","unstructured":"Pankoui Mfonkeu, J. B. et al. Elevated cell-specific microparticles are a biological marker for cerebral dysfunctions in human severe malaria. PLoS One 5, e13415, doi: 10.1371\/journal.pone.0013415 (2010).","journal-title":"PLoS One"},{"key":"BFsrep37871_CR14","doi-asserted-by":"publisher","first-page":"2542","DOI":"10.1001\/jama.291.21.2542-b","volume":"291","author":"V Combes","year":"2004","unstructured":"Combes, V. et al. Circulating endothelial microparticles in malawian children with severe falciparum malaria complicated with coma. JAMA 291, 2542\u20132544, doi: 10.1001\/jama.291.21.2542-b (2004).","journal-title":"JAMA"},{"key":"BFsrep37871_CR15","doi-asserted-by":"publisher","first-page":"700","DOI":"10.1093\/infdis\/jiq104","volume":"203","author":"D Nantakomol","year":"2011","unstructured":"Nantakomol, D. et al. Circulating red cell-derived microparticles in human malaria. J Infect Dis 203, 700\u2013706, doi: 10.1093\/infdis\/jiq104 (2011).","journal-title":"J Infect Dis"},{"key":"BFsrep37871_CR16","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/j.ddmod.2005.05.015","volume":"2","author":"V Combes","year":"2005","unstructured":"Combes, V., de Souza, J. B., R\u00e9nia, L., Hunt, N. H. & Grau, G. E. Cerebral malaria: Which parasite? Which model? Drug Discovery Today: Disease Models 2, 141\u2013147, doi: 10.1016\/j.ddmod.2005.05.015 (2005).","journal-title":"Drug Discovery Today: Disease Models"},{"key":"BFsrep37871_CR17","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1016\/S0002-9440(10)62253-5","volume":"166","author":"V Combes","year":"2005","unstructured":"Combes, V. et al. ABCA1 gene deletion protects against cerebral malaria: potential pathogenic role of microparticles in neuropathology. Am J Pathol 166, 295\u2013302, doi: 10.1016\/S0002-9440(10)62253-5 (2005).","journal-title":"Am J Pathol"},{"key":"BFsrep37871_CR18","doi-asserted-by":"publisher","first-page":"e1003839","DOI":"10.1371\/journal.ppat.1003839","volume":"10","author":"F El-Assaad","year":"2014","unstructured":"El-Assaad, F., Wheway, J., Hunt, N. H., Grau, G. E. & Combes, V. Production, fate and pathogenicity of plasma microparticles in murine cerebral malaria. PLoS Pathog 10, e1003839, doi: 10.1371\/journal.ppat.1003839 (2014).","journal-title":"PLoS Pathog"},{"key":"BFsrep37871_CR19","doi-asserted-by":"publisher","first-page":"1321","DOI":"10.1073\/pnas.0706867105","volume":"105","author":"MF Penet","year":"2008","unstructured":"Penet, M. F. et al. Protection against cerebral malaria by the low-molecular-weight thiol pantethine. Proc Natl Acad Sci USA 105, 1321\u20131326, doi: 10.1073\/pnas.0706867105 (2008).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep37871_CR20","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1016\/j.ijpara.2006.02.005","volume":"36","author":"V Combes","year":"2006","unstructured":"Combes, V., Coltel, N., Faille, D., Wassmer, S. C. & Grau, G. E. Cerebral malaria: role of microparticles and platelets in alterations of the blood-brain barrier. Int J Parasitol 36, 541\u2013546, doi: 10.1016\/j.ijpara.2006.02.005 (2006).","journal-title":"Int J Parasitol"},{"key":"BFsrep37871_CR21","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1016\/j.pneurobio.2010.01.007","volume":"91","author":"V Combes","year":"2010","unstructured":"Combes, V. et al. Microvesiculation and cell interactions at the brain-endothelial interface in cerebral malaria pathogenesis. Prog Neurobiol 91, 140\u2013151, doi: 10.1016\/j.pneurobio.2010.01.007 (2010).","journal-title":"Prog Neurobiol"},{"key":"BFsrep37871_CR22","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1038\/nbt.2839","volume":"32","author":"JA Vizcaino","year":"2014","unstructured":"Vizcaino, J. A. et al. ProteomeXchange provides globally coordinated proteomics data submission and dissemination. Nat Biotechnol 32, 223\u2013226, doi: 10.1038\/nbt.2839 (2014).","journal-title":"Nat Biotechnol"},{"key":"BFsrep37871_CR23","doi-asserted-by":"publisher","first-page":"D1063","DOI":"10.1093\/nar\/gks1262","volume":"41","author":"JA Vizcaino","year":"2013","unstructured":"Vizcaino, J. A. et al. The PRoteomics IDEntifications (PRIDE) database and associated tools: status in 2013. Nucleic Acids Res 41, D1063\u20131069, doi: 10.1093\/nar\/gks1262 (2013).","journal-title":"Nucleic Acids Res"},{"key":"BFsrep37871_CR24","doi-asserted-by":"publisher","first-page":"1047","DOI":"10.1161\/CIRCRESAHA.110.226456","volume":"107","author":"SF Mause","year":"2010","unstructured":"Mause, S. F. & Weber, C. Microparticles: protagonists of a novel communication network for intercellular information exchange. Circ Res 107, 1047\u20131057, doi: 10.1161\/CIRCRESAHA.110.226456 (2010).","journal-title":"Circ Res"},{"key":"BFsrep37871_CR25","doi-asserted-by":"publisher","first-page":"e26588","DOI":"10.1371\/journal.pone.0026588","volume":"6","author":"L Martin-Jaular","year":"2011","unstructured":"Martin-Jaular, L., Nakayasu, E. S., Ferrer, M., Almeida, I. C. & Del Portillo, H. A. Exosomes from Plasmodium yoelii-infected reticulocytes protect mice from lethal infections. PLoS One 6, e26588, doi: 10.1371\/journal.pone.0026588 (2011).","journal-title":"PLoS One"},{"key":"BFsrep37871_CR26","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1016\/j.chom.2013.04.009","volume":"13","author":"PY Mantel","year":"2013","unstructured":"Mantel, P. Y. et al. Malaria-infected erythrocyte-derived microvesicles mediate cellular communication within the parasite population and with the host immune system. Cell Host Microbe 13, 521\u2013534, doi: 10.1016\/j.chom.2013.04.009 (2013).","journal-title":"Cell Host Microbe"},{"key":"BFsrep37871_CR27","doi-asserted-by":"publisher","first-page":"845","DOI":"10.1055\/s-0030-1267038","volume":"36","author":"KM Little","year":"2010","unstructured":"Little, K. M., Smalley, D. M., Harthun, N. L. & Ley, K. The plasma microparticle proteome. Semin Thromb Hemost 36, 845\u2013856, doi: 10.1055\/s-0030-1267038 (2010).","journal-title":"Semin Thromb Hemost"},{"key":"BFsrep37871_CR28","doi-asserted-by":"publisher","first-page":"e269","DOI":"10.1016\/j.thromres.2010.01.019","volume":"125","author":"E Ramacciotti","year":"2010","unstructured":"Ramacciotti, E. et al. Proteomics of microparticles after deep venous thrombosis. Thromb Res 125, e269\u2013274, doi: 10.1016\/j.thromres.2010.01.019 (2010).","journal-title":"Thromb Res"},{"key":"BFsrep37871_CR29","doi-asserted-by":"publisher","first-page":"2154","DOI":"10.1021\/pr200901p","volume":"11","author":"O Ostergaard","year":"2012","unstructured":"Ostergaard, O. et al. Quantitative proteome profiling of normal human circulating microparticles. J Proteome Res 11, 2154\u20132163, doi: 10.1021\/pr200901p (2012).","journal-title":"J Proteome Res"},{"key":"BFsrep37871_CR30","doi-asserted-by":"publisher","first-page":"1604","DOI":"10.1182\/blood-2004-03-1095","volume":"106","author":"I Del Conde","year":"2005","unstructured":"Del Conde, I., Shrimpton, C. N., Thiagarajan, P. & Lopez, J. A. Tissue-factor-bearing microvesicles arise from lipid rafts and fuse with activated platelets to initiate coagulation. Blood 106, 1604\u20131611, doi: 10.1182\/blood-2004-03-1095 (2005).","journal-title":"Blood"},{"key":"BFsrep37871_CR31","doi-asserted-by":"publisher","first-page":"e3694","DOI":"10.1371\/journal.pone.0003694","volume":"3","author":"MP Hunter","year":"2008","unstructured":"Hunter, M. P. et al. Detection of microRNA expression in human peripheral blood microvesicles. PLoS One 3, e3694, doi: 10.1371\/journal.pone.0003694 (2008).","journal-title":"PLoS One"},{"key":"BFsrep37871_CR32","doi-asserted-by":"publisher","first-page":"570","DOI":"10.1016\/j.coph.2013.05.008","volume":"13","author":"LV Norling","year":"2013","unstructured":"Norling, L. V. & Dalli, J. Microparticles are novel effectors of immunity. Curr Opin Pharmacol 13, 570\u2013575, doi: 10.1016\/j.coph.2013.05.008 (2013).","journal-title":"Curr Opin Pharmacol"},{"key":"BFsrep37871_CR33","doi-asserted-by":"publisher","first-page":"534","DOI":"10.1038\/nature10606","volume":"480","author":"C Crosnier","year":"2011","unstructured":"Crosnier, C. et al. Basigin is a receptor essential for erythrocyte invasion by Plasmodium falciparum. Nature 480, 534\u2013537, doi: 10.1038\/nature10606 (2011).","journal-title":"Nature"},{"key":"BFsrep37871_CR34","doi-asserted-by":"publisher","first-page":"e92411","DOI":"10.1371\/journal.pone.0092411","volume":"9","author":"M Brizuela","year":"2014","unstructured":"Brizuela, M. et al. Treatment of erythrocytes with the 2-cys peroxiredoxin inhibitor, Conoidin A, prevents the growth of Plasmodium falciparum and enhances parasite sensitivity to chloroquine. PLoS One 9, e92411, doi: 10.1371\/journal.pone.0092411 (2014).","journal-title":"PLoS One"},{"key":"BFsrep37871_CR35","doi-asserted-by":"publisher","first-page":"12560","DOI":"10.1073\/pnas.0705313104","volume":"104","author":"Y Liu","year":"2007","unstructured":"Liu, Y. et al. Aquaporin 9 is the major pathway for glycerol uptake by mouse erythrocytes, with implications for malarial virulence. Proc Natl Acad Sci USA 104, 12560\u201312564, doi: 10.1073\/pnas.0705313104 (2007).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep37871_CR36","doi-asserted-by":"publisher","first-page":"670","DOI":"10.1111\/j.1365-2141.2011.08755.x","volume":"154","author":"CA Moxon","year":"2011","unstructured":"Moxon, C. A., Grau, G. E. & Craig, A. G. Malaria: modification of the red blood cell and consequences in the human host. Br J Haematol 154, 670\u2013679, doi: 10.1111\/j.1365-2141.2011.08755.x (2011).","journal-title":"Br J Haematol"},{"key":"BFsrep37871_CR37","doi-asserted-by":"publisher","first-page":"3308","DOI":"10.1128\/MCB.9.8.3308","volume":"9","author":"P Fraser","year":"1989","unstructured":"Fraser, P., Cummings, P. & Curtis, P. The mouse carbonic anhydrase I gene contains two tissue-specific promoters. Mol Cell Biol 9, 3308\u20133313 (1989).","journal-title":"Mol Cell Biol"},{"key":"BFsrep37871_CR38","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1016\/j.atherosclerosis.2015.05.012","volume":"241","author":"MM Adeva-Andany","year":"2015","unstructured":"Adeva-Andany, M. M., Fernandez-Fernandez, C., Sanchez-Bello, R., Donapetry-Garcia, C. & Martinez-Rodriguez, J. The role of carbonic anhydrase in the pathogenesis of vascular calcification in humans. Atherosclerosis 241, 183\u2013191, doi: 10.1016\/j.atherosclerosis.2015.05.012 (2015).","journal-title":"Atherosclerosis"},{"key":"BFsrep37871_CR39","doi-asserted-by":"publisher","first-page":"948","DOI":"10.1086\/432941","volume":"41","author":"CR Newton","year":"2005","unstructured":"Newton, C. R. et al. The prognostic value of measures of acid\/base balance in pediatric falciparum malaria, compared with other clinical and laboratory parameters. Clin Infect Dis 41, 948\u2013957, doi: 10.1086\/432941 (2005).","journal-title":"Clin Infect Dis"},{"key":"BFsrep37871_CR40","doi-asserted-by":"publisher","first-page":"686","DOI":"10.4161\/viru.26348","volume":"4","author":"P Cabrales","year":"2013","unstructured":"Cabrales, P. et al. Cerebral tissue oxygenation impairment during experimental cerebral malaria. Virulence 4, 686\u2013697, doi: 10.4161\/viru.26348 (2013).","journal-title":"Virulence"},{"key":"BFsrep37871_CR41","doi-asserted-by":"publisher","first-page":"1150","DOI":"10.1128\/IAI.06008-11","volume":"80","author":"M Krupka","year":"2012","unstructured":"Krupka, M. et al. Mild Plasmodium falciparum malaria following an episode of severe malaria is associated with induction of the interferon pathway in Malawian children. Infect Immun 80, 1150\u20131155, doi: 10.1128\/IAI.06008-11 (2012).","journal-title":"Infect Immun"},{"key":"BFsrep37871_CR42","doi-asserted-by":"publisher","first-page":"e1004038","DOI":"10.1371\/journal.ppat.1004038","volume":"10","author":"J Bachmann","year":"2014","unstructured":"Bachmann, J. et al. Affinity proteomics reveals elevated muscle proteins in plasma of children with cerebral malaria. PLoS Pathog 10, e1004038, doi: 10.1371\/journal.ppat.1004038 (2014).","journal-title":"PLoS Pathog"},{"key":"BFsrep37871_CR43","doi-asserted-by":"publisher","first-page":"42","DOI":"10.4269\/ajtmh.14-0841","volume":"93","author":"SC Wassmer","year":"2015","unstructured":"Wassmer, S. C. et al. Investigating the Pathogenesis of Severe Malaria: A Multidisciplinary and Cross-Geographical Approach. Am J Trop Med Hyg 93, 42\u201356, doi: 10.4269\/ajtmh.14-0841 (2015).","journal-title":"Am J Trop Med Hyg"},{"key":"BFsrep37871_CR44","doi-asserted-by":"publisher","first-page":"e1004528","DOI":"10.1371\/journal.ppat.1004528","volume":"10","author":"A Nacer","year":"2014","unstructured":"Nacer, A. et al. Experimental cerebral malaria pathogenesis\u2013hemodynamics at the blood brain barrier. PLoS Pathog 10, e1004528, doi: 10.1371\/journal.ppat.1004528 (2014).","journal-title":"PLoS Pathog"},{"key":"BFsrep37871_CR45","doi-asserted-by":"publisher","first-page":"e24784","DOI":"10.1371\/journal.pone.0024784","volume":"6","author":"Y Wu","year":"2011","unstructured":"Wu, Y., Szestak, T., Stins, M. & Craig, A. G. Amplification of P. falciparum Cytoadherence through induction of a pro-adhesive state in host endothelium. PLoS One 6, e24784, doi: 10.1371\/journal.pone.0024784 (2011).","journal-title":"PLoS One"},{"key":"BFsrep37871_CR46","doi-asserted-by":"publisher","first-page":"e1004236","DOI":"10.1371\/journal.ppat.1004236","volume":"10","author":"S Pai","year":"2014","unstructured":"Pai, S. et al. Real-time imaging reveals the dynamics of leukocyte behaviour during experimental cerebral malaria pathogenesis. PLoS Pathog 10, e1004236, doi: 10.1371\/journal.ppat.1004236 (2014).","journal-title":"PLoS Pathog"},{"key":"BFsrep37871_CR47","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1016\/j.intimp.2014.12.033","volume":"24","author":"MC Souza","year":"2015","unstructured":"Souza, M. C. et al. Lipoxin A4 attenuates endothelial dysfunction during experimental cerebral malaria. Int Immunopharmacol 24, 400\u2013407, doi: 10.1016\/j.intimp.2014.12.033 (2015).","journal-title":"Int Immunopharmacol"},{"key":"BFsrep37871_CR48","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1097\/MOH.0b013e328345a4cf","volume":"18","author":"H Kim","year":"2011","unstructured":"Kim, H., Higgins, S., Liles, W. C. & Kain, K. C. Endothelial activation and dysregulation in malaria: a potential target for novel therapeutics. Curr Opin Hematol 18, 177\u2013185, doi: 10.1097\/MOH.0b013e328345a4cf (2011).","journal-title":"Curr Opin Hematol"},{"key":"BFsrep37871_CR49","doi-asserted-by":"crossref","unstructured":"O\u2019Regan, N. et al. A novel role for von Willebrand factor in the pathogenesis of experimental cerebral malaria. Blood, doi: 10.1182\/blood-2015-07-654921 (2015).","DOI":"10.1182\/blood-2015-07-654921"},{"key":"BFsrep37871_CR50","doi-asserted-by":"publisher","first-page":"610","DOI":"10.1093\/infdis\/jit419","volume":"209","author":"CA Moxon","year":"2014","unstructured":"Moxon, C. A. et al. Persistent endothelial activation and inflammation after Plasmodium falciparum Infection in Malawian children. J Infect Dis 209, 610\u2013615, doi: 10.1093\/infdis\/jit419 (2014).","journal-title":"J Infect Dis"},{"key":"BFsrep37871_CR51","doi-asserted-by":"publisher","first-page":"1623","DOI":"10.1007\/s00018-003-2347-x","volume":"60","author":"F Gimenez","year":"2003","unstructured":"Gimenez, F., Barraud de Lagerie, S., Fernandez, C., Pino, P. & Mazier, D. Tumor necrosis factor alpha in the pathogenesis of cerebral malaria. Cell Mol Life Sci 60, 1623\u20131635, doi: 10.1007\/s00018-003-2347-x (2003).","journal-title":"Cell Mol Life Sci"},{"key":"BFsrep37871_CR52","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1111\/j.1462-5822.2010.01528.x","volume":"13","author":"SC Wassmer","year":"2011","unstructured":"Wassmer, S. C. et al. Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF. Cell Microbiol 13, 198\u2013209, doi: 10.1111\/j.1462-5822.2010.01528.x (2011).","journal-title":"Cell Microbiol"},{"key":"BFsrep37871_CR53","doi-asserted-by":"publisher","first-page":"1180","DOI":"10.4049\/jimmunol.176.2.1180","volume":"176","author":"SC Wassmer","year":"2006","unstructured":"Wassmer, S. C. et al. TGF-beta1 released from activated platelets can induce TNF-stimulated human brain endothelium apoptosis: a new mechanism for microvascular lesion during cerebral malaria. J Immunol 176, 1180\u20131184 (2006).","journal-title":"J Immunol"},{"key":"BFsrep37871_CR54","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1159\/000330095","volume":"4","author":"G Srikrishna","year":"2012","unstructured":"Srikrishna, G. S100A8 and S100A9: new insights into their roles in malignancy. J Innate Immun 4, 31\u201340, doi: 10.1159\/000330095 (2012).","journal-title":"J Innate Immun"},{"key":"BFsrep37871_CR55","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1385\/1-59259-183-3:069","volume":"172","author":"CW Heizmann","year":"2002","unstructured":"Heizmann, C. W. The multifunctional S100 protein family. Methods Mol Biol 172, 69\u201380, doi: 10.1385\/1-59259-183-3:069 (2002).","journal-title":"Methods Mol Biol"},{"key":"BFsrep37871_CR56","doi-asserted-by":"publisher","first-page":"821","DOI":"10.1007\/s00726-010-0528-0","volume":"41","author":"J Goyette","year":"2011","unstructured":"Goyette, J. & Geczy, C. L. Inflammation-associated S100 proteins: new mechanisms that regulate function. Amino Acids 41, 821\u2013842, doi: 10.1007\/s00726-010-0528-0 (2011).","journal-title":"Amino Acids"},{"key":"BFsrep37871_CR57","doi-asserted-by":"publisher","first-page":"628","DOI":"10.1002\/1529-0131(200003) 43:3<628::AID-ANR20>3.0.CO;2-X","volume":"43","author":"M Frosch","year":"2000","unstructured":"Frosch, M. et al. Myeloid-related proteins 8 and 14 are specifically secreted during interaction of phagocytes and activated endothelium and are useful markers for monitoring disease activity in pauciarticular-onset juvenile rheumatoid arthritis. Arthritis Rheum 43, 628\u2013637, doi: 10.1002\/1529-0131(200003) 43:3&lt;628::AID-ANR20&gt;3.0.CO;2-X (2000).","journal-title":"Arthritis Rheum"},{"key":"BFsrep37871_CR58","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1111\/j.1365-2133.2006.07198.x","volume":"155","author":"S Benoit","year":"2006","unstructured":"Benoit, S. et al. Elevated serum levels of calcium-binding S100 proteins A8 and A9 reflect disease activity and abnormal differentiation of keratinocytes in psoriasis. Br J Dermatol 155, 62\u201366, doi: 10.1111\/j.1365-2133.2006.07198.x (2006).","journal-title":"Br J Dermatol"},{"key":"BFsrep37871_CR59","doi-asserted-by":"publisher","first-page":"1622","DOI":"10.1016\/j.bcp.2006.05.017","volume":"72","author":"C Gebhardt","year":"2006","unstructured":"Gebhardt, C., Nemeth, J., Angel, P. & Hess, J. S100A8 and S100A9 in inflammation and cancer. Biochem Pharmacol 72, 1622\u20131631, doi: 10.1016\/j.bcp.2006.05.017 (2006).","journal-title":"Biochem Pharmacol"},{"key":"BFsrep37871_CR60","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1128\/cdli.4.4.435-439.1997","volume":"4","author":"G Bordmann","year":"1997","unstructured":"Bordmann, G. et al. MRP 8\/14 as marker for Plasmodium falciparum-induced malaria episodes in individuals in a holoendemic area. Clin Diagn Lab Immunol 4, 435\u2013439 (1997).","journal-title":"Clin Diagn Lab Immunol"},{"key":"BFsrep37871_CR61","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.exppara.2014.01.003","volume":"138","author":"H Mizobuchi","year":"2014","unstructured":"Mizobuchi, H. et al. The accumulation of macrophages expressing myeloid-related protein 8 (MRP8) and MRP14 in the spleen of BALB\/cA mice during infection with Plasmodium berghei. Exp Parasitol 138, 1\u20138, doi: 10.1016\/j.exppara.2014.01.003 (2014).","journal-title":"Exp Parasitol"},{"key":"BFsrep37871_CR62","doi-asserted-by":"publisher","first-page":"2955","DOI":"10.1182\/blood-2004-07-2520","volume":"105","author":"D Viemann","year":"2005","unstructured":"Viemann, D. et al. Myeloid-related proteins 8 and 14 induce a specific inflammatory response in human microvascular endothelial cells. Blood 105, 2955\u20132962, doi: 10.1182\/blood-2004-07-2520 (2005).","journal-title":"Blood"},{"key":"BFsrep37871_CR63","doi-asserted-by":"publisher","first-page":"9496","DOI":"10.1074\/jbc.272.14.9496","volume":"272","author":"A Rammes","year":"1997","unstructured":"Rammes, A. et al. Myeloid-related protein (MRP) 8 and MRP14, calcium-binding proteins of the S100 family, are secreted by activated monocytes via a novel, tubulin-dependent pathway. J Biol Chem 272, 9496\u20139502 (1997).","journal-title":"J Biol Chem"},{"key":"BFsrep37871_CR64","doi-asserted-by":"publisher","first-page":"e1001942","DOI":"10.1371\/journal.pmed.1001942","volume":"13","author":"I Chen","year":"2016","unstructured":"Chen, I. et al. \u201cAsymptomatic\u201d Malaria: A Chronic and Debilitating Infection That Should Be Treated. PLoS Med 13, e1001942, doi: 10.1371\/journal.pmed.1001942 (2016).","journal-title":"PLoS Med"},{"key":"BFsrep37871_CR65","doi-asserted-by":"publisher","first-page":"291","DOI":"10.1016\/S1286-4579(02)01541-1","volume":"4","author":"JB de Souza","year":"2002","unstructured":"de Souza, J. B. & Riley, E. M. Cerebral malaria: the contribution of studies in animal models to our understanding of immunopathogenesis. Microbes Infect 4, 291\u2013300 (2002).","journal-title":"Microbes Infect"},{"key":"BFsrep37871_CR66","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1093\/cid\/ciu735","volume":"60","author":"E Shabani","year":"2015","unstructured":"Shabani, E. et al. High plasma erythropoietin levels are associated with prolonged coma duration and increased mortality in children with cerebral malaria. Clin Infect Dis 60, 27\u201335, doi: 10.1093\/cid\/ciu735 (2015).","journal-title":"Clin Infect Dis"},{"key":"BFsrep37871_CR67","doi-asserted-by":"publisher","first-page":"3378","DOI":"10.4049\/jimmunol.1303431","volume":"193","author":"J Wheway","year":"2014","unstructured":"Wheway, J., Latham, S. L., Combes, V. & Grau, G. E. Endothelial microparticles interact with and support the proliferation of T cells. J Immunol 193, 3378\u20133387, doi: 10.4049\/jimmunol.1303431 (2014).","journal-title":"J Immunol"},{"key":"BFsrep37871_CR68","doi-asserted-by":"publisher","first-page":"672","DOI":"10.1096\/fj.12-216531","volume":"27","author":"SL Latham","year":"2013","unstructured":"Latham, S. L. et al. Cooperation between beta- and gamma-cytoplasmic actins in the mechanical regulation of endothelial microparticle formation. FASEB J 27, 672\u2013683, doi: 10.1096\/fj.12-216531 (2013).","journal-title":"FASEB J"},{"key":"BFsrep37871_CR69","doi-asserted-by":"publisher","first-page":"1146","DOI":"10.1039\/c3mb70544g","volume":"10","author":"L Comelli","year":"2014","unstructured":"Comelli, L. et al. Characterization of secreted vesicles from vascular smooth muscle cells. Mol Biosyst 10, 1146\u20131152, doi: 10.1039\/c3mb70544g (2014).","journal-title":"Mol Biosyst"},{"key":"BFsrep37871_CR70","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1006\/abio.1998.2809","volume":"263","author":"CL Gatlin","year":"1998","unstructured":"Gatlin, C. L., Kleemann, G. R., Hays, L. G., Link, A. J. & Yates, J. R. 3rd Protein identification at the low femtomole level from silver-stained gels using a new fritless electrospray interface for liquid chromatography-microspray and nanospray mass spectrometry. Anal Biochem 263, 93\u2013101, doi: 10.1006\/abio.1998.2809 (1998).","journal-title":"Anal Biochem"},{"key":"BFsrep37871_CR71","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1016\/j.jprot.2012.12.012","volume":"79","author":"F Gluck","year":"2013","unstructured":"Gluck, F. et al. EasyProt\u2013an easy-to-use graphical platform for proteomics data analysis. J Proteomics 79, 146\u2013160, doi: 10.1016\/j.jprot.2012.12.012 (2013).","journal-title":"J Proteomics"},{"key":"BFsrep37871_CR72","doi-asserted-by":"publisher","first-page":"2783","DOI":"10.1074\/mcp.M110.001008","volume":"9","author":"N Tiberti","year":"2010","unstructured":"Tiberti, N. et al. Discovery and verification of osteopontin and Beta-2-microglobulin as promising markers for staging human African trypanosomiasis. Mol Cell Proteomics 9, 2783\u20132795, doi: 10.1074\/mcp.M110.001008 (2010).","journal-title":"Mol Cell Proteomics"},{"key":"BFsrep37871_CR73","doi-asserted-by":"publisher","first-page":"176","DOI":"10.3858\/emm.2008.40.2.176","volume":"40","author":"H Savli","year":"2008","unstructured":"Savli, H., Szendroi, A., Romics, I. & Nagy, B. Gene network and canonical pathway analysis in prostate cancer: a microarray study. Exp Mol Med 40, 176\u2013185, doi: 10.3858\/emm.2008.40.2.176 (2008).","journal-title":"Exp Mol Med"},{"key":"BFsrep37871_CR74","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1186\/s12918-015-0167-x","volume":"9","author":"CC Reyes-Gibby","year":"2015","unstructured":"Reyes-Gibby, C. C., Yuan, C., Wang, J., Yeung, S. C. & Shete, S. Gene network analysis shows immune-signaling and ERK1\/2 as novel genetic markers for multiple addiction phenotypes: alcohol, smoking and opioid addiction. BMC Syst Biol 9, 25, doi: 10.1186\/s12918-015-0167-x (2015).","journal-title":"BMC Syst Biol"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep37871.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep37871","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep37871.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T02:46:51Z","timestamp":1672886811000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep37871"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,5]]},"references-count":74,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,12,23]]}},"alternative-id":["BFsrep37871"],"URL":"https:\/\/doi.org\/10.1038\/srep37871","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,5]]},"assertion":[{"value":"6 June 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 October 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 December 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"37871"}}