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TRIP-1 lacks a signal peptide, therefore, in this study, we provide evidence that intracellular TRIP-1 can be packaged and exported to the ECM via exosomes. Overexpression of TRIP-1 in MC3T3-E1 cells resulted in increased matrix mineralization during differentiation and knockdown resulted in reduced effects.\n                    <jats:italic>In vivo<\/jats:italic>\n                    function of TRIP-1 was studied by an implantation assay performed using TRIP-1 overexpressing and knockdown cells cultured in a 3-dimmensional scaffold. After 4 weeks, the subcutaneous tissues from TRIP-1 overexpressing cells showed higher calcium and phosphate deposits, arranged collagen fibrils and increased expression of Runx2 and alkaline phosphatase. Nucleation studies on demineralized and deproteinized dentin wafer is a powerful tool to determine the functional role of noncollagenous proteins in matrix mineralization. Using this system, we provide evidence that TRIP-1 binds to Type-I collagen and can promote mineralization. Surface plasmon resonance analysis demonstrated that TRIP-1 binds to collagen with\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>D<\/jats:italic>\n                    <\/jats:sub>\n                    \u2009=\u200948\u2009\u03bcM. SEM and TEM analysis showed that TRIP-1 promoted the nucleation and growth of calcium phosphate mineral aggregates. Taken together, we provide mechanistic insights of this intracellular protein in matrix mineralization.\n                  <\/jats:p>","DOI":"10.1038\/srep37885","type":"journal-article","created":{"date-parts":[[2016,11,24]],"date-time":"2016-11-24T05:39:38Z","timestamp":1479965978000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["TGF beta receptor II interacting protein-1, an intracellular protein has an extracellular role as a modulator of matrix mineralization"],"prefix":"10.1038","volume":"6","author":[{"given":"Amsaveni","family":"Ramachandran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sriram","family":"Ravindran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Chieh","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"George","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,11,24]]},"reference":[{"key":"BFsrep37885_CR1","doi-asserted-by":"publisher","first-page":"1576","DOI":"10.1002\/jbmr.1611","volume":"27","author":"D Metz-Estrella","year":"2012","unstructured":"Metz-Estrella, D., Jonason, J. 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