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A possible alternative to invasive or non-invasive sampling of wild-living vertebrates is the use of vertebrate material contained in invertebrates feeding on them, their feces, or their remains. Carrion flies have been shown to contain vertebrate DNA; here we investigate whether they might also be suitable for wildlife pathogen detection. We collected 498 flies in Ta\u00ef National Park, C\u00f4te d\u2019Ivoire, a tropical rainforest and examined them for adenoviruses (family <jats:italic>Adenoviridae<\/jats:italic>), whose DNA is frequently shed in feces of local mammals. Adenoviral DNA was detected in 6\/142 mammal-positive flies. Phylogenetic analyses revealed that five of these sequences were closely related to sequences obtained from local non-human primates, while the sixth sequence was closely related to a murine adenovirus. Next-generation sequencing-based DNA-profiling of the meals of the respective flies identified putative hosts that were a good fit to those suggested by adenoviral sequence affinities. We conclude that, while characterizing the genetic diversity of wildlife infectious agents through fly-based monitoring may not be cost-efficient, this method could probably be used to detect the genetic material of wildlife infectious agents causing wildlife mass mortality in pristine areas.<\/jats:p>","DOI":"10.1038\/srep37952","type":"journal-article","created":{"date-parts":[[2016,11,30]],"date-time":"2016-11-30T10:20:08Z","timestamp":1480501208000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Assessing the feasibility of fly based surveillance of wildlife infectious diseases"],"prefix":"10.1038","volume":"6","author":[{"given":"Constanze","family":"Hoffmann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Stockhausen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Merkel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Calvignac-Spencer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian H.","family":"Leendertz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,11,30]]},"reference":[{"key":"BFsrep37952_CR1","doi-asserted-by":"crossref","first-page":"1369","DOI":"10.1126\/science.1259657","volume":"345","author":"SK Gire","year":"2014","unstructured":"Gire, S. 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