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We have previously shown that N-WASP is critical for cell-ECM adhesion in fibroblasts. To characterize the role of N-WASP in fibroblast for skin development, we generated a conditional knockout mouse model in which fibroblast N-WASP was ablated using the Cre recombinase driven by Fibroblast Specific Protein promoter (Fsp-Cre). N-WASP<jats:sup>FKO<\/jats:sup> (<jats:italic>N-WASP<\/jats:italic><jats:sup><jats:italic>fl\/fl<\/jats:italic><\/jats:sup><jats:italic>; Fsp-cre<\/jats:italic>) were born following Mendelian genetics, survived without any visible abnormalities for more than 1 year and were sexually reproductive, suggesting that expression of N-WASP in fibroblast is not critical for survival under laboratory conditions. Histological sections of N-WASP<jats:sup>FKO<\/jats:sup> mice skin (13 weeks old) showed thicker epidermis with higher percentage of cells staining for proliferation marker (PCNA), suggesting that N-WASP deficient fibroblasts promote keratinocyte proliferation. N-WASP<jats:sup>FKO<\/jats:sup> mice skin had elevated collagen content, elevated expression of FGF7 (keratinocyte growth factor) and TGF\u03b2 signaling proteins. Wound healing was faster in N-WASP<jats:sup>FKO<\/jats:sup> mice compared to control mice and N-WASP deficient fibroblasts were found to have enhanced collagen gel contraction properties. These results suggest that N-WASP deficiency in fibroblasts improves wound healing by growth factor-mediated enhancement of keratinocyte proliferation and increased wound contraction in mice.<\/jats:p>","DOI":"10.1038\/srep38109","type":"journal-article","created":{"date-parts":[[2016,12,2]],"date-time":"2016-12-02T09:59:52Z","timestamp":1480672792000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Conditional knockout of N-WASP in mouse fibroblast caused keratinocyte hyper proliferation and enhanced wound closure"],"prefix":"10.1038","volume":"6","author":[{"given":"Neeraj","family":"Jain","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pazhanichamy","family":"Kalailingam","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai Wei","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui Bing","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming Keat","family":"Sng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy Soon Kiat","family":"Chan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nguan Soon","family":"Tan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thirumaran","family":"Thanabalu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,12,2]]},"reference":[{"key":"BFsrep38109_CR1","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1101\/gad.1086903","volume":"17","author":"L Alonso","year":"2003","unstructured":"Alonso, L. & Fuchs, E. 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