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We hypothesized that platelets accumulate early on in ischemic myocardium and a newly developed technology of non-invasive molecular PET imaging of activated platelets can thus detect minimal degrees of myocardial ischemia. To induce different degrees of minimal cardiac ischemia, the left anterior descending artery (LAD) was ligated for 10, 20 or 60\u2009min. Mice were injected with a newly generated scFv<jats:sub>anti-GPIIb\/IIIa<\/jats:sub>-<jats:sup>64<\/jats:sup>CuMeCOSar radiotracer, composed of a single-chain antibody that only binds to activated integrin GPIIb\/IIIa (\u03b1<jats:sub>IIb<\/jats:sub>\u03b2<jats:sub>III<\/jats:sub>) and thus to activated platelets, and a sarcophagine cage MeCOSar complexing the long half-life PET tracer copper-64. A single PET\/CT scan was performed. Evans Blue\/TTC staining to detect necrosis as well as classical serological biomarkers like Troponin I and heart-type fatty acid-binding protein (H-FABP) were negative, whereas PET imaging of activated platelets was able to detect small degrees of ischemia. 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