{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T05:52:58Z","timestamp":1788587578673,"version":"build-2803163510"},"reference-count":18,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2016,12,2]],"date-time":"2016-12-02T00:00:00Z","timestamp":1480636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2016,12,2]],"date-time":"2016-12-02T00:00:00Z","timestamp":1480636800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Group B\n                    <jats:italic>Streptococcus<\/jats:italic>\n                    (GBS) is an encapsulated, gram-positive pathogen that is an important cause of neonatal invasive infections, including sepsis and meningitis. There are ten known GBS serotypes based on distinct capsule compositions (Ia, Ib, II-IX), and current candidate capsular polysaccharide conjugate vaccines target only a subset of these. Serotyping of GBS isolates is important for understanding local epidemiology and for monitoring for serotype replacement or capsular switching. However, serotyping generally requires either latex agglutination, multiplex PCR with analysis of band sizes, or analysis of whole genome sequences\u2013all techniques that are either expensive or not widely available. Here we report the development of a robust real-time PCR assay for determining GBS serotypes. Using both a diverse reference set of strains encompassing all ten serotypes and a collection of clinical isolates, we demonstrate concordance between real-time PCR serotyping and latex agglutination. We propose that real-time PCR serotyping represents an attractive alternative to current serotyping methods and may allow for improved acquisition of GBS serotype data.\n                  <\/jats:p>","DOI":"10.1038\/srep38523","type":"journal-article","created":{"date-parts":[[2016,12,2]],"date-time":"2016-12-02T05:31:39Z","timestamp":1480656699000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Real-time PCR-based serotyping of Streptococcus agalactiae"],"prefix":"10.1038","volume":"6","author":[{"given":"Kathleen M.","family":"Breeding","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhavana","family":"Ragipani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun-Uk David","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Malik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tara M.","family":"Randis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam J.","family":"Ratner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,12,2]]},"reference":[{"key":"BFsrep38523_CR1","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1016\/S0140-6736(11)61651-6","volume":"379","author":"KM Edmond","year":"2012","unstructured":"Edmond, K. M. et al. Group B streptococcal disease in infants aged younger than 3 months: systematic review and meta-analysis. Lancet 379, 547\u2013556 (2012).","journal-title":"Lancet"},{"key":"BFsrep38523_CR2","doi-asserted-by":"publisher","first-page":"D7","DOI":"10.1016\/j.vaccine.2013.01.009","volume":"31","author":"K Le Doare","year":"2013","unstructured":"Le Doare, K. & Heath, P. T. An overview of global GBS epidemiology. Vaccine 31, D7\u2013D12 (2013).","journal-title":"Vaccine"},{"key":"BFsrep38523_CR3","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1056\/NEJM200001063420103","volume":"342","author":"SJ Schrag","year":"2000","unstructured":"Schrag, S. J. et al. Group B streptococcal disease in the era of intrapartum antibiotic prophylaxis. N Engl J Med 342, 15\u201320 (2000).","journal-title":"N Engl J Med"},{"key":"BFsrep38523_CR4","unstructured":"Verani, J. R., McGee, L. & Schrag, S. J. Division of Bacterial Diseases National Center for Immunization and Respiratory Diseases Centers for Disease Control and Prevention CDC. Prevention of perinatal group B streptococcal disease\u2013revised guidelines from CDC, 2010. MMWR Recomm Rep 59, 1\u201336 (2010)."},{"key":"BFsrep38523_CR5","doi-asserted-by":"publisher","first-page":"817","DOI":"10.1542\/peds.2010-2217","volume":"127","author":"BJ Stoll","year":"2011","unstructured":"Stoll, B. J. et al. Early Onset Neonatal Sepsis: The Burden of Group B Streptococcal and E. coli Disease Continues. Pediatrics 127, 817\u2013826 (2011).","journal-title":"Pediatrics"},{"key":"BFsrep38523_CR6","doi-asserted-by":"publisher","first-page":"828","DOI":"10.1097\/AOG.0000000000000163","volume":"123","author":"JR Verani","year":"2014","unstructured":"Verani, J. R. et al. Early-onset group B streptococcal disease in the United States: potential for further reduction. Obstetrics and gynecology 123, 828\u2013837 (2014).","journal-title":"Obstetrics and gynecology"},{"key":"BFsrep38523_CR7","doi-asserted-by":"publisher","first-page":"2308","DOI":"10.1172\/JCI119042","volume":"98","author":"DL Kasper","year":"1996","unstructured":"Kasper, D. L. et al. Immune response to type III group B streptococcal polysaccharide-tetanus toxoid conjugate vaccine. J Clin Invest 98, 2308\u20132314 (1996).","journal-title":"J Clin Invest"},{"key":"BFsrep38523_CR8","doi-asserted-by":"crossref","unstructured":"Madhi, S. A. et al. Safety and immunogenicity of an investigational maternal trivalent group B streptococcus vaccine in healthy women and their infants: a randomised phase 1b\/2 trial. Lancet Infect Dis. doi: 10.1016\/S1473-3099(16)00152-3 (2016).","DOI":"10.1016\/S1473-3099(16)00152-3"},{"key":"BFsrep38523_CR9","doi-asserted-by":"publisher","first-page":"23437","DOI":"10.1074\/jbc.M114.567974","volume":"289","author":"F Berti","year":"2014","unstructured":"Berti, F. et al. Structure of the type IX group B Streptococcus capsular polysaccharide and its evolutionary relationship with types V and VII. J Biol Chem 289, 23437\u201323448 (2014).","journal-title":"J Biol Chem"},{"key":"BFsrep38523_CR10","doi-asserted-by":"publisher","first-page":"3096","DOI":"10.1128\/IAI.73.5.3096-3103.2005","volume":"73","author":"MJ Cieslewicz","year":"2005","unstructured":"Cieslewicz, M. J. et al. Structural and genetic diversity of group B streptococcus capsular polysaccharides. 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J Bacteriol 192, 1361\u20131369 (2010).","journal-title":"J Bacteriol"},{"key":"BFsrep38523_CR13","doi-asserted-by":"publisher","first-page":"1985","DOI":"10.1128\/JCM.00159-07","volume":"45","author":"C Poyart","year":"2007","unstructured":"Poyart, C. et al. Multiplex PCR assay for rapid and accurate capsular typing of group B streptococci. J Clin Microbiol 45, 1985\u20131988 (2007).","journal-title":"J Clin Microbiol"},{"key":"BFsrep38523_CR14","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1016\/j.mimet.2009.11.010","volume":"80","author":"M Imperi","year":"2010","unstructured":"Imperi, M. et al. A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae. J. Microbiol. Methods 80, 212\u2013214 (2010).","journal-title":"J. Microbiol. 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Chemother"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep38523.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep38523","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep38523.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,4]],"date-time":"2023-01-04T20:03:25Z","timestamp":1672862605000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep38523"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12,2]]},"references-count":18,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2016,12,23]]}},"alternative-id":["BFsrep38523"],"URL":"https:\/\/doi.org\/10.1038\/srep38523","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12,2]]},"assertion":[{"value":"16 May 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 November 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 December 2016","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"38523"}}