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Therefore, allograft sterilisation is crucial. We aimed to determine whether polyvinylpyrrolidone-iodine (PVP-I) can be used for sterilisation and as a new wet-preservation method. PVP-I\u2013sterilised and preserved allografts demonstrated improved mechanical property, osteogenesis, and excellent microbial inhibition. A thigh muscle pouch model of nude mice showed that PVP-I\u2013preserved allografts demonstrated better ectopic formation than Co<jats:sub>60<\/jats:sub>-sterilised allografts (control) <jats:italic>in vivo<\/jats:italic> (P\u2009&lt;\u20090.05). Furthermore, the PVP-I\u2013preserved group showed no difference between 24\u2009h and 12 weeks of allograft preservation (P\u2009&gt;\u20090.05). PVP-I\u2013preserved allografts showed more hydrophilic surfaces and PVP-I\u2013sterilised tendons showed higher mechanical strength than Co<jats:sub>60<\/jats:sub>-sterilised tendons (P\u2009&lt;\u20090.05). The level of residual PVP-I was higher without washing and with prolonged preservation (P\u2009&lt;\u20090.05). <jats:italic>In vitro<\/jats:italic> cellular tests showed that appropriate PVP-I concentration was nontoxic to preosteoblast cells, and cellular differentiation measured by alkaline phosphatase activity and osteogenic gene markers was enhanced (P\u2009&lt;\u20090.05). Therefore, the improved biological performance of implanted allografts may be attributable to better surface properties and residual PVP-I, and PVP-I immersion can be a simple, easy method for allograft sterilisation and preservation.<\/jats:p>","DOI":"10.1038\/srep38669","type":"journal-article","created":{"date-parts":[[2016,12,9]],"date-time":"2016-12-09T10:38:46Z","timestamp":1481279926000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Use of polyvinylpyrrolidone-iodine solution for sterilisation and preservation improves mechanical properties and osteogenesis of allografts"],"prefix":"10.1038","volume":"6","author":[{"given":"Yantao","family":"Zhao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiantong","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhonghai","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuli","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Xia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuxun","family":"Hou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongbin","family":"Zhong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feimin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Gu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,12,9]]},"reference":[{"key":"BFsrep38669_CR1","doi-asserted-by":"crossref","first-page":"22106","DOI":"10.1038\/srep22106","volume":"6","author":"G Chen","year":"2016","unstructured":"Chen, G. et al. 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