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The target protein of azoles is CYP51, a lanosterol 14-\u03b1-demethylase involved in the biosynthesis of ergosterol. Recently, a novel azole resistance mechanism has arisen in pathogenic fungal species <jats:italic>Aspergillus fumigatus<\/jats:italic>. Resistant strains contain a 34-bp or 46-bp tandem repeat (TR) in the promoter of <jats:italic>cyp51A<\/jats:italic>, and have disseminated globally in a short period of time. In this study, we investigated whether an azole-resistant strain with a 46-bp TR (TR46\/Y121F\/T289A) could be sensitised to azoles by deletion of <jats:italic>srbA<\/jats:italic>, encoding a direct regulator of <jats:italic>cyp51A<\/jats:italic>. The loss of SrbA did not affect colony growth or conidia production, but decreased expression of <jats:italic>cyp51A<\/jats:italic>. The <jats:italic>srbA<\/jats:italic> deletion strain showed hyper-susceptibility to medical azoles as well as azole fungicides, while its sensitivity to non-azole fungicides was unchanged. This is the first demonstration that deletion of a regulator of <jats:italic>cyp51A<\/jats:italic> can sensitise an azole-resistant <jats:italic>A. fumigatus<\/jats:italic> strain. This finding may assist in the development of new drugs to help combat life-threatening azole-resistant fungal pathogens.<\/jats:p>","DOI":"10.1038\/srep38833","type":"journal-article","created":{"date-parts":[[2016,12,9]],"date-time":"2016-12-09T13:03:52Z","timestamp":1481288632000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Sensitisation of an Azole-Resistant Aspergillus fumigatus Strain containing the Cyp51A-Related Mutation by Deleting the SrbA Gene"],"prefix":"10.1038","volume":"6","author":[{"given":"Daisuke","family":"Hagiwara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsuhiko","family":"Kamei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,12,9]]},"reference":[{"key":"BFsrep38833_CR1","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.medmal.2013.02.010","volume":"43","author":"L Leli\u00e8vre","year":"2013","unstructured":"Leli\u00e8vre, L. et al. 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