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Despite its tiny body architecture and limited trafficking machinery, <jats:italic>T. gondii<\/jats:italic> relies heavily on transport of vesicles containing proteins, lipids and important virulence-like factors that are delivered to these secretory organelles. However, our understanding on how trafficking of vesicles operates in the parasite is still limited. Here, we show that the <jats:italic>T. gondii<\/jats:italic> vacuolar protein sorting 9 (<jats:italic>Tg<\/jats:italic>Vps9), has guanine nucleotide exchange factor (GEF) activity towards Rab5a and is crucial for sorting of proteins destined to secretory organelles. Our results illuminate features of <jats:italic>Tg<\/jats:italic>Vps9 protein as a key trafficking facilitator that regulates protein maturation, secretory organelle formation and secretion, thereby ensuring a primary role in host infection by <jats:italic>T. gondii<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/srep38842","type":"journal-article","created":{"date-parts":[[2016,12,14]],"date-time":"2016-12-14T10:38:02Z","timestamp":1481711882000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["A Critical Role for Toxoplasma gondii Vacuolar Protein Sorting VPS9 in Secretory Organelle Biogenesis and Host Infection"],"prefix":"10.1038","volume":"6","author":[{"given":"Takaya","family":"Sakura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabien","family":"Sindikubwabo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lena K.","family":"Oesterlin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo","family":"Bousquet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Slomianny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed-Ali","family":"Hakimi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon","family":"Langsley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanislas","family":"Tomavo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,12,14]]},"reference":[{"key":"BFsrep38842_CR1","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1038\/nrmicro1800","volume":"6","author":"JC Boothroyd","year":"2008","unstructured":"Boothroyd, J. 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