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A promising target is the \u03b2-ketoacyl-acyl carrier protein synthase FabF, which participates in the catalysis of fatty acid synthesis and elongation, and is required for the production of phospholipid membranes, lipoproteins, and lipopolysaccharides. In this study, we report the 1.35\u2009\u00c5 crystal structure of FabF from <jats:italic>L. monocytogenes<\/jats:italic>, providing an excellent platform for the rational design of novel inhibitors. By comparing the structure of <jats:italic>L. monocytogenes<\/jats:italic> FabF with other published bacterial FabF structures in complex with known inhibitors and substrates, we highlight conformational changes within the active site, which will need to be accounted for during drug design and virtual screening studies. This high-resolution structure of FabF represents an important step in the development of new classes of antimicrobial agents targeting FabF for the treatment of listeriosis.<\/jats:p>","DOI":"10.1038\/srep39277","type":"journal-article","created":{"date-parts":[[2017,1,3]],"date-time":"2017-01-03T11:33:37Z","timestamp":1483443217000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Structural characterisation of the fatty acid biosynthesis enzyme FabF from the pathogen Listeria monocytogenes"],"prefix":"10.1038","volume":"7","author":[{"given":"Tatiana P.","family":"Soares da Costa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey D.","family":"Nanson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jade K.","family":"Forwood","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,1,3]]},"reference":[{"key":"BFsrep39277_CR1","doi-asserted-by":"crossref","first-page":"672","DOI":"10.5694\/j.1326-5377.1996.tb138683.x","volume":"165","author":"SK Crerar","year":"1996","unstructured":"Crerar, S. 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