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We investigated the roles of hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) and cysteine (Cys) in plant responses to Cd\n                    <jats:sup>2+<\/jats:sup>\n                    stress. The expression of H\n                    <jats:sub>2<\/jats:sub>\n                    S synthetic genes\n                    <jats:italic>LCD<\/jats:italic>\n                    and\n                    <jats:italic>DES1<\/jats:italic>\n                    were induced by Cd\n                    <jats:sup>2+<\/jats:sup>\n                    within 3\u2009h, and endogenous H\n                    <jats:sub>2<\/jats:sub>\n                    S was then rapidly released. H\n                    <jats:sub>2<\/jats:sub>\n                    S promoted the expression of Cys synthesis-related genes\n                    <jats:italic>SAT1<\/jats:italic>\n                    and\n                    <jats:italic>OASA1<\/jats:italic>\n                    , which led to endogenous Cys accumulation. The H\n                    <jats:sub>2<\/jats:sub>\n                    S and Cys cycle system was stimulated by Cd\n                    <jats:sup>2+<\/jats:sup>\n                    stress, and it maintained high levels in plant cells. H\n                    <jats:sub>2<\/jats:sub>\n                    S inhibited the ROS burst by inducing alternative respiration capacity (AP) and antioxidase activity. H\n                    <jats:sub>2<\/jats:sub>\n                    S weakened Cd\n                    <jats:sup>2+<\/jats:sup>\n                    toxicity by inducing the metallothionein (MTs) genes expression. Cys promoted GSH accumulation and inhibited the ROS burst, and GSH induced the expression of phytochelatin (PCs) genes, counteracting Cd\n                    <jats:sup>2+<\/jats:sup>\n                    toxicity. In summary, the H\n                    <jats:sub>2<\/jats:sub>\n                    S and Cys cycle system played a key role in plant responses to Cd\n                    <jats:sup>2+<\/jats:sup>\n                    stress. The Cd\n                    <jats:sup>2+<\/jats:sup>\n                    tolerance was weakened when the cycle system was blocked in\n                    <jats:italic>lcddes1-1<\/jats:italic>\n                    and\n                    <jats:italic>oasa1<\/jats:italic>\n                    mutants. This paper is the first to describe the role of the H\n                    <jats:sub>2<\/jats:sub>\n                    S and Cys cycle system in Cd\n                    <jats:sup>2+<\/jats:sup>\n                    stress and to explore the relevant and specificity mechanisms of H\n                    <jats:sub>2<\/jats:sub>\n                    S and Cys in mediating Cd\n                    <jats:sup>2+<\/jats:sup>\n                    stress.\n                  <\/jats:p>","DOI":"10.1038\/srep39702","type":"journal-article","created":{"date-parts":[[2016,12,22]],"date-time":"2016-12-22T07:14:32Z","timestamp":1482390872000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":126,"title":["Hydrogen sulfide - cysteine cycle system enhances cadmium tolerance through alleviating cadmium-induced oxidative stress and ion toxicity in Arabidopsis roots"],"prefix":"10.1038","volume":"6","author":[{"given":"Honglei","family":"Jia","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhua","family":"Dou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wantong","family":"Si","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaolin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiejun","family":"Xi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jisheng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2016,12,22]]},"reference":[{"key":"BFsrep39702_CR1","first-page":"183","volume":"119","author":"NS Bolan","year":"2013","unstructured":"Bolan, N. 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