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To study the T cell-specific function of A20 in bacterial infection, we infected T cell-specific A20 knockout (CD4-Cre A20\n                    <jats:sup>fl\/fl<\/jats:sup>\n                    ) and control mice with\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    . A20-deficient pathogen-specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells expanded stronger resulting in improved pathogen control at day 7 p.i. Imaging flow cytometry revealed that A20-deficient\n                    <jats:italic>Listeria<\/jats:italic>\n                    -specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells underwent increased apoptosis and necroptosis resulting in reduced numbers of memory CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells. In contrast, the primary CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cell response was A20-independent. Upon secondary infection, the increase and function of pathogen-specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, as well as pathogen control were significantly impaired in CD4-Cre A20\n                    <jats:sup>fl\/fl<\/jats:sup>\n                    mice.\n                    <jats:italic>In vitro<\/jats:italic>\n                    , apoptosis and necroptosis of\n                    <jats:italic>Listeria<\/jats:italic>\n                    -specific A20-deficient CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells were strongly induced as demonstrated by increased caspase-3\/7 activity, RIPK1\/RIPK3 complex formation and more morphologically apoptotic and necroptotic CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells.\n                    <jats:italic>In vitro<\/jats:italic>\n                    , A20 limited CD95L and TNF-induced caspase3\/7 activation. In conclusion, T cell-specific A20 limited the expansion but reduced apoptosis and necroptosis of\n                    <jats:italic>Listeria<\/jats:italic>\n                    -specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, resulting in an impaired pathogen control in primary but improved clearance in secondary infection.\n                  <\/jats:p>","DOI":"10.1038\/srep39796","type":"journal-article","created":{"date-parts":[[2016,12,22]],"date-time":"2016-12-22T07:46:14Z","timestamp":1482392774000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["A20 Curtails Primary but Augments Secondary CD8+ T Cell Responses in Intracellular Bacterial Infection"],"prefix":"10.1038","volume":"6","author":[{"given":"Sissy","family":"Just","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gopala","family":"Nishanth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f6rn H.","family":"Buchbinder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Naumann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inna","family":"Lavrik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk","family":"Schl\u00fcter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2016,12,22]]},"reference":[{"key":"BFsrep39796_CR1","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1016\/j.immuni.2011.07.010","volume":"35","author":"N Zhang","year":"2011","unstructured":"Zhang, N. & Bevan, M. 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