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Two millennia after swiping over Europe and North Africa, plague established permanent foci in North Africa but not in neighboring Europe. Mapping human plague foci reported in North Africa for 70 years indicated a significant location at &lt;3 kilometers from the Mediterranean seashore or the edge of salted lakes named chotts. In Algeria, culturing 352 environmental specimens naturally containing 0.5 to 70\u2009g\/L NaCl yielded one <jats:italic>Y. pestis<\/jats:italic> Orientalis biotype isolate in a 40\u2009g\/L NaCl chott soil specimen. Core genome SNP analysis placed this isolate within the <jats:italic>Y. pestis<\/jats:italic> branch 1, Orientalis biovar. Culturing <jats:italic>Y. pestis<\/jats:italic> in broth steadily enriched in NaCl indicated survival up to 150\u2009g\/L NaCl as L-form variants exhibiting a distinctive matrix assisted laser desorption-ionization time-of-flight mass spectrometry peptide profile. Further transcriptomic analyses found the upregulation of several outer-membrane proteins including TolC efflux pump and OmpF porin implied in osmotic pressure regulation. Salt tolerance of <jats:italic>Y. pestis<\/jats:italic> L-form may play a role in the maintenance of natural plague foci in North Africa and beyond, as these geographical correlations could be extended to 31 plague foci in the northern hemisphere (from 15\u00b0N to 50\u00b0N).<\/jats:p>","DOI":"10.1038\/srep40022","type":"journal-article","created":{"date-parts":[[2017,1,5]],"date-time":"2017-01-05T11:01:56Z","timestamp":1483614116000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Yersinia pestis halotolerance illuminates plague reservoirs"],"prefix":"10.1038","volume":"7","author":[{"given":"Maliya Alia","family":"Malek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Idir","family":"Bitam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony","family":"Levasseur","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Terras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Gaudart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Said","family":"Azza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Flaudrops","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Robert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Raoult","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Drancourt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,1,5]]},"reference":[{"key":"BFsrep40022_CR1","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.jinf.2012.09.010","volume":"66","author":"D Raoult","year":"2013","unstructured":"Raoult, D., Mouffok, N., Bitam, I., Piarroux, R. & Drancourt, M. 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