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In particular, the domestic pig is a proven model of human physiology and an agriculturally important species. In this study, we utilized the CRISPR\/Cas9 system to edit the<jats:italic>NANOS2<\/jats:italic>gene in pig embryos to generate offspring with mono-allelic and bi-allelic mutations. We found that<jats:italic>NANOS2<\/jats:italic>knockout pigs phenocopy knockout mice with male specific germline ablation but other aspects of testicular development are normal. Moreover, male pigs with one intact<jats:italic>NANOS2<\/jats:italic>allele and female knockout pigs are fertile. From an agriculture perspective,<jats:italic>NANOS2<\/jats:italic>knockout male pigs are expected to serve as an ideal surrogate for transplantation of donor spermatogonial stem cells to expand the availability of gametes from genetically desirable sires.<\/jats:p>","DOI":"10.1038\/srep40176","type":"journal-article","created":{"date-parts":[[2017,1,10]],"date-time":"2017-01-10T10:46:04Z","timestamp":1484045164000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":120,"title":["Generation of germline ablated male pigs by CRISPR\/Cas9 editing of the NANOS2 gene"],"prefix":"10.1038","volume":"7","author":[{"given":"Ki-Eun","family":"Park","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy V.","family":"Kaucher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Powell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Salman","family":"Waqas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelley E.S.","family":"Sandmaier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa J.","family":"Oatley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Hun","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"Tibary","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Donovan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le Ann","family":"Blomberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon G.","family":"Lillico","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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