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This aspect was investigated, using multi-color flow cytometry, during oral infection of mice with<jats:italic>Salmonella<\/jats:italic>Typhimurium (<jats:italic>S<\/jats:italic>. Typhimurium). The major highlights are: First, a block in the developmental pathway of CD4<jats:sup>\u2212<\/jats:sup>CD8<jats:sup>\u2212<\/jats:sup>double negative (DN) thymocytes is observed. Second, CD4<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup>double positive (DP) thymocytes, mainly in the DP1 (CD5<jats:sup>lo<\/jats:sup>CD3<jats:sup>lo<\/jats:sup>) and DP2 (CD5<jats:sup>hi<\/jats:sup>CD3<jats:sup>int<\/jats:sup>), but not DP3 (CD5<jats:sup>int<\/jats:sup>CD3<jats:sup>hi<\/jats:sup>), subsets are reduced. Third, single positive (SP) thymocytes are more resistant to depletion but their maturation is delayed, leading to accumulation of CD24<jats:sup>hi<\/jats:sup>CD3<jats:sup>hi<\/jats:sup>SP. Kinetic studies during infection demonstrated differences in sensitivity of thymic subpopulations: Immature single positive (ISP)\u2009&gt;\u2009DP1, DP2\u2009&gt;\u2009DN3, DN4\u2009&gt;\u2009DN2\u2009&gt;\u2009CD4<jats:sup>+<\/jats:sup>\u2009&gt;\u2009CD8<jats:sup>+<\/jats:sup>. Upon infection, glucocorticoids (GC), inflammatory cytokines, e.g. Ifn\u03b3, etc are induced, which enhance thymocyte death. Treatment with RU486, the GC receptor antagonist, increases the survival of most thymic subsets during infection. Studies with<jats:italic>Ifn\u03b3<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>mice demonstrated that endogenous Ifn\u03b3 produced during infection enhances the depletion of DN2-DN4 subsets, promotes the accumulation of DP3 and delays the maturation of SP thymocytes. The implications of these observations on host cellular responses during infections are discussed.<\/jats:p>","DOI":"10.1038\/srep40793","type":"journal-article","created":{"date-parts":[[2017,1,16]],"date-time":"2017-01-16T12:23:29Z","timestamp":1484569409000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Differential susceptibility and maturation of thymocyte subsets during Salmonella Typhimurium infection: insights on the roles of glucocorticoids and Interferon-gamma"],"prefix":"10.1038","volume":"7","author":[{"given":"Shamik","family":"Majumdar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mukta","family":"Deobagkar-Lele","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vasista","family":"Adiga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abinaya","family":"Raghavan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nitin","family":"Wadhwa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Syed Moiz","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Supriya Rajendra","family":"Rananaware","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Subhashish","family":"Chakraborty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omana","family":"Joy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dipankar","family":"Nandi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,1,16]]},"reference":[{"key":"BFsrep40793_CR1","doi-asserted-by":"publisher","first-page":"284","DOI":"10.4049\/jimmunol.1000679","volume":"185","author":"MA Yui","year":"2010","unstructured":"Yui, M. 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