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The point mutation at codon 76 (K76T), which has been observed in more than 91% of<jats:italic>P. falciparum<\/jats:italic>isolates in India, is the major determinant of CQ resistance. To overcome the limitations and challenges of traditional methods, in this investigation we developed an easy to use loop mediated isothermal amplification (LAMP) protocol for rapid detection of the K76T mutation associated with CQ resistance in<jats:italic>P. falciparum<\/jats:italic>with naked eye visualization. In- house designed primers were synthesized and optimized to specifically distinguish the CQ resistant mutants of<jats:italic>P. falciparum.<\/jats:italic>The LAMP reaction was optimal at 61\u2009\u00b0C for 60\u2009min and calcein dye was added prior to amplification to enable visual detection. We demonstrate the detection limit of &lt;2\u2009ng\/\u03bcl respectively, supporting the high sensitivity of this calcein based LAMP method. To the best of our knowledge this is the first report on the establishment of an easy, reliable and cost effective LAMP assay for rapid and specific detection of highly CQ resistance in<jats:italic>P. falciparum<\/jats:italic>malaria.<\/jats:p>","DOI":"10.1038\/srep41119","type":"journal-article","created":{"date-parts":[[2017,1,30]],"date-time":"2017-01-30T10:33:17Z","timestamp":1485772397000},"update-policy":"http:\/\/dx.doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Establishment and application of a novel isothermal amplification assay for rapid detection of chloroquine resistance (K76T) in Plasmodium falciparum"],"prefix":"10.1038","volume":"7","author":[{"given":"Madhvi","family":"Chahar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neelima","family":"Mishra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anup","family":"Anvikar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajnikant","family":"Dixit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neena","family":"Valecha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,1,30]]},"reference":[{"key":"BFsrep41119_CR1","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1016\/S0140-6736(12)60034-8","volume":"379","author":"CJ Murray","year":"2012","unstructured":"Murray, C. 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