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Antibiotic therapy often needs to be administered for long durations in acne therapy, which results in extensive antibiotic exposure. The present study investigated a new treatment model for evaluating the antibacterial effects of lysozyme (LY)-shelled microbubbles (MBs) and ultrasound (US)-mediated LY-shelled MBs cavitation against <jats:italic>P. acnes<\/jats:italic> both <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>, with the aims of reducing the dose and treatment duration and improving the prognosis of acne vulgaris. In terms of the <jats:italic>in vitro<\/jats:italic> treatment efficacy, the growth of <jats:italic>P. acnes<\/jats:italic> was inhibited by 86.08\u2009\u00b1\u20092.99% in the LY-shelled MBs group and by 57.74\u2009\u00b1\u20093.09% in the LY solution group. For US power densities of 1, 2, and 3\u2009W\/cm<jats:sup>2<\/jats:sup> in the LY-shelled MBs group, the growth of <jats:italic>P. acnes<\/jats:italic> was inhibited by 95.79\u2009\u00b1\u20093.30%, 97.99\u2009\u00b1\u20091.16%, and 98.69\u2009\u00b1\u20091.13%, respectively. The <jats:italic>in vivo<\/jats:italic> results showed that the recovery rate on day 13 was higher in the US group with LY-shelled MBs (97.8\u2009\u00b1\u200919.8%) than in the LY-shelled MBs group (90.3\u2009\u00b1\u200923.3%). Our results show that combined treatments of US and LY-shelled MBs can significantly reduce the treatment duration and inhibit <jats:italic>P.-acnes<\/jats:italic>-induced inflammatory skin diseases.<\/jats:p>","DOI":"10.1038\/srep41325","type":"journal-article","created":{"date-parts":[[2017,1,24]],"date-time":"2017-01-24T11:12:02Z","timestamp":1485256322000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Treatment effects of lysozyme-shelled microbubbles and ultrasound in inflammatory skin disease"],"prefix":"10.1038","volume":"7","author":[{"given":"Ai-Ho","family":"Liao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chi-Ray","family":"Hung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chieh-Fu","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Chun","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang-Kang","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,1,24]]},"reference":[{"key":"BFsrep41325_CR1","doi-asserted-by":"publisher","first-page":"13","DOI":"10.2147\/AHMT.S55832","volume":"7","author":"DD Lynn","year":"2016","unstructured":"Lynn, D. 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