{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T05:29:52Z","timestamp":1785648592409,"version":"3.56.0"},"reference-count":50,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,1,31]],"date-time":"2017-01-31T00:00:00Z","timestamp":1485820800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,1,31]],"date-time":"2017-01-31T00:00:00Z","timestamp":1485820800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Researchers in the field of mitochondrial biology are increasingly unveiling of the complex mechanisms between mitochondrial dysfunction and noncoding RNAs (ncRNAs). However, roles of ncRNAs underlying mitochondrial myopathy remain unexplored. The aim of this study was to elucidate the regulating networks of dysregulated ncRNAs in Mitochondrial myopathy, Encephalopathy, Lactic Acidosis, and Stroke-like episodes (MELAS) with mitochondrial DNA (mtDNA) A3243G mutation, which might make contributions to the unveiling of the complex mechanisms underlying mitochondrial myopathy and, possibly, new tools applicable to clinical practice. Through high-throughput technology followed by quantitative real-time polymerase chain reaction (qRT-PCR) and bioinformatics analyses, for the first time, we found that the dysregulated muscle miRNAs and lncRNAs between 20 MELAS patients with mtDNA A3243G mutation and 20 controls formed complex regulation networks and participated in immune system, signal transduction, translation, muscle contraction and other pathways in discovery and training phase. Then, selected ncRNAs were validated in muscle and serum in independent validation cohorts by qRT-PCR. Finally, ROC curve analysis indicated reduced serum miR-27b-3p had the better diagnosis value than lactate and might serve as a novel, noninvasive biomarker for MELAS. Follow-up investigation is warranted to better understand roles of ncRNAs in mitochondrial myopathy pathogenesis.<\/jats:p>","DOI":"10.1038\/srep41639","type":"journal-article","created":{"date-parts":[[2017,1,31]],"date-time":"2017-01-31T11:13:37Z","timestamp":1485861217000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Identification of miRNA, lncRNA and mRNA-associated ceRNA networks and potential biomarker for MELAS with mitochondrial DNA A3243G mutation"],"prefix":"10.1038","volume":"7","author":[{"given":"Wei","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Zhuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunqian","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuying","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanzhu","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,1,31]]},"reference":[{"key":"BFsrep41639_CR1","doi-asserted-by":"publisher","first-page":"1494","DOI":"10.1126\/science.aac7516","volume":"349","author":"RN Lightowlers","year":"2015","unstructured":"Lightowlers, R. N., Taylor, R. W. & Turnbull, D. M. Mutations causing mitochondrial disease: What is new and what challenges remain? Science. 349, 1494\u20131499 (2015).","journal-title":"Science"},{"key":"BFsrep41639_CR2","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1159\/000446586","volume":"7","author":"DM Niyazov","year":"2001","unstructured":"Niyazov, D. M., Kahler, S. G. & Frye, R. E. Primary Mitochondrial Disease and Secondary Mitochondrial Dysfunction: Importance of Distinction for Diagnosis and Treatment. Molecular syndromology. 7, 122\u2013137 (20016).","journal-title":"Molecular syndromology"},{"key":"BFsrep41639_CR3","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1002\/ana.24362","volume":"77","author":"GS Gorman","year":"2015","unstructured":"Gorman, G. S. et al. Prevalence of nuclear and mitochondrial DNA mutations related to adult mitochondrial disease. Annals of neurology. 77, 753\u2013759 (2015).","journal-title":"Annals of neurology"},{"key":"BFsrep41639_CR4","doi-asserted-by":"crossref","unstructured":"Johnson, S. C. et al. Network analysis of mitonuclear GWAS reveals functional networks and tissue expression profiles of disease-associated genes. Human genetics, doi: 10.1007\/s00439-016-1736-9 (2016).","DOI":"10.1007\/s00439-016-1736-9"},{"key":"BFsrep41639_CR5","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.mito.2016.07.003","volume":"30","author":"M Picard","year":"2016","unstructured":"Picard, M., Wallace, D. C. & Burelle, Y. The rise of mitochondria in medicine. Mitochondrion. 30, 105\u2013116 (2016).","journal-title":"Mitochondrion"},{"key":"BFsrep41639_CR6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1600-0404.2007.00836.x","volume":"116","author":"J Finsterer","year":"2007","unstructured":"Finsterer, J. Genetic, pathogenetic, and phenotypic implications of the mitochondrial A3243G tRNALeu(UUR) mutation. Acta neurologica Scandinavica. 116, 1\u201314 (2007).","journal-title":"Acta neurologica Scandinavica"},{"key":"BFsrep41639_CR7","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1007\/s40572-016-0103-2","volume":"3","author":"L Lambertini","year":"2016","unstructured":"Lambertini, L. & Byun, H. M. Mitochondrial Epigenetics and Environmental Exposure. Current environmental health reports. 3, 214\u2013224 (2016).","journal-title":"Current environmental health reports"},{"key":"BFsrep41639_CR8","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1038\/nrg3722","volume":"15","author":"KV Morris","year":"2014","unstructured":"Morris, K. V. & Mattick, J. S. The rise of regulatory RNA. Nature reviews. Genetics. 15, 423\u2013437 (2014).","journal-title":"Nature reviews. Genetics"},{"key":"BFsrep41639_CR9","doi-asserted-by":"publisher","first-page":"R57","DOI":"10.1186\/gb-2014-15-4-r57","volume":"15","author":"MR Friedlander","year":"2014","unstructured":"Friedlander, M. R. et al. Evidence for the biogenesis of more than 1,000 novel human microRNAs. Genome biology. 15, R57 (2014).","journal-title":"Genome biology"},{"key":"BFsrep41639_CR10","doi-asserted-by":"publisher","first-page":"e1003569","DOI":"10.1371\/journal.pgen.1003569","volume":"9","author":"MJ Hangauer","year":"2013","unstructured":"Hangauer, M. J., Vaughn, I. W. & McManus, M. T. Pervasive transcription of the human genome produces thousands of previously unidentified long intergenic noncoding RNAs. PLoS genetics. 9, e1003569 (2013).","journal-title":"PLoS genetics"},{"key":"BFsrep41639_CR11","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1007\/s00109-014-1131-8","volume":"92","author":"PG Maass","year":"2014","unstructured":"Maass, P. G., Luft, F. C. & Bahring, S. Long non-coding RNA in health and disease. Journal of molecular medicine. 92, 337\u2013346 (2014).","journal-title":"Journal of molecular medicine"},{"key":"BFsrep41639_CR12","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1007\/978-3-319-22671-2_8","volume":"888","author":"FV Duarte","year":"2015","unstructured":"Duarte, F. V., Palmeira, C. M. & Rolo, A. P. The Emerging Role of MitomiRs in the Pathophysiology of Human Disease. Advances in experimental medicine and biology. 888, 123\u2013154 (2015).","journal-title":"Advances in experimental medicine and biology"},{"key":"BFsrep41639_CR13","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1016\/j.mito.2012.10.009","volume":"12","author":"L Sripada","year":"2012","unstructured":"Sripada, L., Tomar, D. & Singh, R. Mitochondria: one of the destinations of miRNAs. Mitochondrion. 12, 593\u2013599 (2012).","journal-title":"Mitochondrion"},{"key":"BFsrep41639_CR14","doi-asserted-by":"publisher","first-page":"1441","DOI":"10.1016\/j.bbagen.2013.09.002","volume":"1840","author":"M Tomasetti","year":"2014","unstructured":"Tomasetti, M., Neuzil, J. & Dong, L. MicroRNAs as regulators of mitochondrial function: role in cancer suppression. Biochimica et biophysica acta. 1840, 1441\u20131453 (2014).","journal-title":"Biochimica et biophysica acta"},{"key":"BFsrep41639_CR15","doi-asserted-by":"publisher","first-page":"24895","DOI":"10.3390\/ijms161024895","volume":"16","author":"Y Hu","year":"2015","unstructured":"Hu, Y., Deng, H., Xu, S. & Zhang, J. MicroRNAs Regulate Mitochondrial Function in Cerebral Ischemia-Reperfusion Injury. International journal of molecular sciences. 16, 24895\u201324917 (2015).","journal-title":"International journal of molecular sciences"},{"key":"BFsrep41639_CR16","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1101\/gad.276022.115","volume":"30","author":"JH Noh","year":"2016","unstructured":"Noh, J. H. et al. HuR and GRSF1 modulate the nuclear export and mitochondrial localization of the lncRNA RMRP. Genes & development. 30, 1224\u20131239 (2016).","journal-title":"Genes & development"},{"key":"BFsrep41639_CR17","doi-asserted-by":"publisher","first-page":"e1004467","DOI":"10.1371\/journal.pgen.1004467","volume":"10","author":"K Wang","year":"2014","unstructured":"Wang, K. et al. MDRL lncRNA regulates the processing of miR-484 primary transcript by targeting miR-361. PLoS genetics. 10, e1004467 (2014).","journal-title":"PLoS genetics"},{"key":"BFsrep41639_CR18","doi-asserted-by":"publisher","first-page":"2172","DOI":"10.1080\/15548627.2015.1106663","volume":"11","author":"S Huang","year":"2015","unstructured":"Huang, S. et al. A new microRNA signal pathway regulated by long noncoding RNA TGFB2-OT1 in autophagy and inflammation of vascular endothelial cells. Autophagy. 11, 2172\u20132183 (2015).","journal-title":"Autophagy"},{"key":"BFsrep41639_CR19","doi-asserted-by":"publisher","first-page":"3596","DOI":"10.1038\/ncomms4596","volume":"5","author":"K Wang","year":"2014","unstructured":"Wang, K. et al. CARL lncRNA inhibits anoxia-induced mitochondrial fission and apoptosis in cardiomyocytes by impairing miR-539-dependent PHB2 downregulation. Nature communications. 5, 3596 (2014).","journal-title":"Nature communications"},{"key":"BFsrep41639_CR20","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.1093\/jjco\/hyv132","volume":"45","author":"HJ Li","year":"2015","unstructured":"Li, H. J. et al. Long non-coding RNA UCA1 promotes glutamine metabolism by targeting miR-16 in human bladder cancer. Japanese journal of clinical oncology. 45, 1055\u20131063 (2015).","journal-title":"Japanese journal of clinical oncology"},{"key":"BFsrep41639_CR21","doi-asserted-by":"publisher","first-page":"866","DOI":"10.1096\/fj.04-3045fje","volume":"19","author":"M Crimi","year":"2005","unstructured":"Crimi, M. et al. Skeletal muscle gene expression profiling in mitochondrial disorders. FASEB journal: official publication of the Federation of American Societies for Experimental Biology. 19, 866\u2013868 (2005).","journal-title":"FASEB journal: official publication of the Federation of American Societies for Experimental Biology"},{"key":"BFsrep41639_CR22","doi-asserted-by":"publisher","first-page":"e69282","DOI":"10.1371\/journal.pone.0069282","volume":"8","author":"Z Zhang","year":"2013","unstructured":"Zhang, Z. et al. Primary respiratory chain disease causes tissue-specific dysregulation of the global transcriptome and nutrient-sensing signaling network. PloS one. 8, e69282 (2013).","journal-title":"PloS one."},{"key":"BFsrep41639_CR23","doi-asserted-by":"publisher","first-page":"315","DOI":"10.1016\/j.ymeth.2014.06.003","volume":"69","author":"Z Zhang","year":"2014","unstructured":"Zhang, Z., Hailat, Z., Falk, M. J. & Chen, X. W. Integrative analysis of independent transcriptome data for rare diseases. Methods. 69, 315\u2013325 (2014).","journal-title":"Methods"},{"key":"BFsrep41639_CR24","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1186\/1471-2164-15-91","volume":"15","author":"SG Kalko","year":"2014","unstructured":"Kalko, S. G. et al. Transcriptomic profiling of TK2 deficient human skeletal muscle suggests a role for the p53 signalling pathway and identifies growth and differentiation factor-15 as a potential novel biomarker for mitochondrial myopathies. BMC genomics. 15, 91 (2014).","journal-title":"BMC genomics"},{"key":"BFsrep41639_CR25","doi-asserted-by":"publisher","first-page":"106","DOI":"10.1016\/j.biocel.2014.02.012","volume":"50","author":"Z Zhang","year":"2014","unstructured":"Zhang, Z. & Falk, M. J. Integrated transcriptome analysis across mitochondrial disease etiologies and tissues improves understanding of common cellular adaptations to respiratory chain dysfunction. The international journal of biochemistry & cell biology. 50, 106\u2013111 (2014).","journal-title":"The international journal of biochemistry & cell biology"},{"key":"BFsrep41639_CR26","doi-asserted-by":"publisher","first-page":"246","DOI":"10.7150\/ijbs.11138","volume":"11","author":"YE Lee","year":"2015","unstructured":"Lee, Y. E., Hong, C. Y., Lin, Y. L. & Chen, R. M. MicroRNA-1 participates in nitric oxide-induced apoptotic insults to MC3T3-E1 cells by targeting heat-shock protein-70. International journal of biological sciences. 11, 246\u2013255 (2015).","journal-title":"International journal of biological sciences"},{"key":"BFsrep41639_CR27","doi-asserted-by":"publisher","first-page":"484","DOI":"10.18632\/aging.100905","volume":"8","author":"A Lang","year":"2016","unstructured":"Lang, A. et al. MicroRNA-15b regulates mitochondrial ROS production and the senescence-associated secretory phenotype through sirtuin 4\/SIRT4. Aging. 8, 484\u2013505 (2016).","journal-title":"Aging"},{"key":"BFsrep41639_CR28","doi-asserted-by":"publisher","first-page":"13027","DOI":"10.1073\/pnas.0805038105","volume":"105","author":"E van Rooij","year":"2008","unstructured":"van Rooij, E. et al. Dysregulation of microRNAs after myocardial infarction reveals a role of miR-29 in cardiac fibrosis. Proceedings of the National Academy of Sciences of the United States of America. 105, 13027\u20131303 (2008).","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"BFsrep41639_CR29","doi-asserted-by":"publisher","first-page":"129","DOI":"10.3892\/mmr.2016.5208","volume":"14","author":"XC Zeng","year":"2016","unstructured":"Zeng, X. C., Li, L., Wen, H. & Bi, Q. MicroRNA-128 inhibition attenuates myocardial ischemia\/reperfusion injury-induced cardiomyocyte apoptosis by the targeted activation of peroxisome proliferator-activated receptor gamma. Molecular medicine reports. 14, 129\u2013136 (2016).","journal-title":"Molecular medicine reports"},{"key":"BFsrep41639_CR30","doi-asserted-by":"crossref","unstructured":"Nie, Y. et al. Impaired exercise tolerance, mitochondrial biogenesis, and muscle fiber maintenance in miR-133a-deficient mice. FASEB journal: official publication of the Federation of American Societies for Experimental Biology. pii: fj.201600529R (2016).","DOI":"10.1096\/fj.201600529R"},{"key":"BFsrep41639_CR31","doi-asserted-by":"publisher","first-page":"720","DOI":"10.1016\/j.bbrc.2013.05.050","volume":"435","author":"MJ Cha","year":"2013","unstructured":"Cha, M. J. et al. MicroRNA-145 suppresses ROS-induced Ca2+ overload of cardiomyocytes by targeting CaMKIIdelta. Biochemical and biophysical research communications. 435, 720\u2013726 (2013).","journal-title":"Biochemical and biophysical research communications"},{"key":"BFsrep41639_CR32","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1016\/j.expneurol.2014.12.018","volume":"265","author":"WX Wang","year":"2015","unstructured":"Wang, W. X. et al. Mitochondria-associated microRNAs in rat hippocampus following traumatic brain injury. Experimental neurology. 265, 84\u201393 (2015).","journal-title":"Experimental neurology."},{"key":"BFsrep41639_CR33","doi-asserted-by":"publisher","first-page":"534","DOI":"10.1152\/physiolgenomics.00130.2010","volume":"43","author":"Y Ye","year":"2011","unstructured":"Ye, Y. et al. The role of microRNA in modulating myocardial ischemia-reperfusion injury. Physiological genomics. 43, 534\u2013542 (2011).","journal-title":"Physiological genomics."},{"key":"BFsrep41639_CR34","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1038\/jcbfm.2008.157","volume":"29","author":"A Dharap","year":"2009","unstructured":"Dharap, A., Bowen, K., Place, R., Li, L. C. & Vemuganti, R. Transient focal ischemia induces extensive temporal changes in rat cerebral microRNAome. Journal of cerebral blood flow and metabolism: official journal of the International Society of Cerebral Blood Flow and Metabolism. 29, 675\u2013687 (2009).","journal-title":"Journal of cerebral blood flow and metabolism: official journal of the International Society of Cerebral Blood Flow and Metabolism"},{"key":"BFsrep41639_CR35","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1182\/blood-2004-01-0240","volume":"105","author":"PG Smith","year":"2005","unstructured":"Smith, P. G. et al. The phosphodiesterase PDE4B limits cAMP-associated PI3K\/AKT-dependent apoptosis in diffuse large B-cell lymphoma. Blood. 105, 308\u2013316 (2005).","journal-title":"Blood"},{"key":"BFsrep41639_CR36","doi-asserted-by":"publisher","first-page":"e44907","DOI":"10.1371\/journal.pone.0044907","volume":"7","author":"R Li","year":"2012","unstructured":"Li, R. et al. MicroRNA-145 protects cardiomyocytes against hydrogen peroxide (H2O2)-induced apoptosis through targeting the mitochondria apoptotic pathway. PloS one. 7, e44907 (2012).","journal-title":"PloS one"},{"key":"BFsrep41639_CR37","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1016\/j.bbrc.2015.08.090","volume":"465","author":"S Bucha","year":"2015","unstructured":"Bucha, S., Mukhopadhyay, D. & Bhattacharyya, N. P. Regulation of mitochondrial morphology and cell cycle by microRNA-214 targeting Mitofusin2. Biochemical and biophysical research communications. 465, 797\u2013802 (2015).","journal-title":"Biochemical and biophysical research communications"},{"key":"BFsrep41639_CR38","doi-asserted-by":"publisher","first-page":"1222","DOI":"10.1172\/JCI59327","volume":"122","author":"I Ahmed","year":"2012","unstructured":"Ahmed, I. et al. MicroRNA-214 protects the mouse heart from ischemic injury by controlling Ca2+ overload and cell death. The Journal of clinical investigation. 122, 1222\u20131232 (2012).","journal-title":"The Journal of clinical investigation"},{"key":"BFsrep41639_CR39","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1038\/nature12986","volume":"505","author":"Y Tay","year":"2014","unstructured":"Tay, Y., Rinn, J. & Pandolfi, P. P. The multilayered complexity of ceRNA crosstalk and competition. Nature. 505, 344\u2013352 (2014).","journal-title":"Nature"},{"key":"BFsrep41639_CR40","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1016\/j.freeradbiomed.2013.12.016","volume":"68","author":"N Rastogi","year":"2014","unstructured":"Rastogi, N. et al. (6)-Gingerolinduced myeloid leukemia cell death is initiated by reactive oxygen species and activation of miR-27b expression. Free radical biology & medicine. 68, 288\u2013301 (2014).","journal-title":"Free radical biology & medicine"},{"key":"BFsrep41639_CR41","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1016\/j.bbrc.2015.03.057","volume":"460","author":"R Bi","year":"2015","unstructured":"Bi, R. et al. MicroRNA-27b plays a role in pulmonary arterial hypertension by modulating peroxisome proliferator-activated receptor gamma dependent Hsp90-eNOS signaling and nitric oxide production. Biochemical and biophysical research communications. 460, 469\u2013475 (2015).","journal-title":"Biochemical and biophysical research communications"},{"key":"BFsrep41639_CR42","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1007\/s13105-015-0439-3","volume":"71","author":"X Zhou","year":"2015","unstructured":"Zhou, X., Zuo, S. & Xin, W. miR-27b overexpression improves mitochondrial function in a Sirt1-dependent manner. Journal of physiology and biochemistry. 71, 753\u2013762 (2015).","journal-title":"Journal of physiology and biochemistry"},{"key":"BFsrep41639_CR43","doi-asserted-by":"publisher","first-page":"e1635","DOI":"10.7717\/peerj.1635","volume":"4","author":"X Zhao","year":"2016","unstructured":"Zhao, X. et al. miR-27b attenuates apoptosis induced by transmissible gastroenteritis virus (TGEV) infection via targeting runt-related transcription factor 1 (RUNX1). PeerJ. 4, e1635 (2016).","journal-title":"PeerJ"},{"key":"BFsrep41639_CR44","doi-asserted-by":"crossref","first-page":"19666","DOI":"10.18632\/oncotarget.7531","volume":"7","author":"F Liu","year":"2016","unstructured":"Liu, F. et al. MicroRNA-27b up-regulated by human papillomavirus 16 E7 promotes proliferation and suppresses apoptosis by targeting polo-like kinase2 in cervical cancer. Oncotarget. 7, 19666\u201319679 (2016).","journal-title":"Oncotarget"},{"key":"BFsrep41639_CR45","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1186\/s13024-016-0121-4","volume":"11","author":"J Kim","year":"2016","unstructured":"Kim, J. et al. miR-27a and miR-27b regulate autophagic clearance of damaged mitochondria by targeting PTEN-induced putative kinase 1 (PINK1). Molecular neurodegeneration. 11, 55 (2016).","journal-title":"Molecular neurodegeneration"},{"key":"BFsrep41639_CR46","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1111\/cpr.12277","volume":"49","author":"D Yao","year":"2016","unstructured":"Yao, D. et al. Deconvoluting the complexity of microRNAs in autophagy to improve potential cancer therapy. Cell proliferation. 49, 541\u2013553 (2016).","journal-title":"Cell proliferation"},{"key":"BFsrep41639_CR47","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1159\/000117269","volume":"36","author":"UA Walker","year":"1996","unstructured":"Walker, U. A., Collins, S. & Byrne, E. Respiratory chain encephalomyopathies: a diagnostic classification. European neurology. 36, 260\u2013267 (1996).","journal-title":"European neurology."},{"key":"BFsrep41639_CR48","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1007\/978-1-59745-304-2_19","volume":"382","author":"Q Zhu","year":"2007","unstructured":"Zhu, Q. et al. microParaflo biochip for nucleic acid and protein analysis. Methods in molecular biology. 382, 287\u2013312 (2007).","journal-title":"Methods in molecular biology"},{"key":"BFsrep41639_CR49","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1093\/bioinformatics\/19.2.185","volume":"19","author":"BM Bolstad","year":"2003","unstructured":"Bolstad, B. M., Irizarry, R. A., Astrand, M. & Speed, T. P. A comparison of normalization methods for high density oligonucleotide array data based on variance and bias. Bioinformatics. 19, 185\u2013193 (2003).","journal-title":"Bioinformatics"},{"key":"BFsrep41639_CR50","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1007\/978-1-62703-709-9_10","volume":"1097","author":"PF Stadler","year":"2014","unstructured":"Stadler, P. F. Class-specific prediction of ncRNAs. Methods in molecular biology. 1097, 199\u2013213 (2014).","journal-title":"Methods in molecular biology"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep41639.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep41639","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep41639.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T20:58:29Z","timestamp":1671829109000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep41639"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,31]]},"references-count":50,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,3,14]]}},"alternative-id":["BFsrep41639"],"URL":"https:\/\/doi.org\/10.1038\/srep41639","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,31]]},"assertion":[{"value":"18 October 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 December 2016","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"31 January 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"41639"}}