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Although the thromboxane-prostanoid receptor (TP) is proposed to mediate such a response of PGI<jats:sub>2<\/jats:sub>, other unknown receptor(s) might also be involved. TP knockout (TP<jats:sup>\u2212\/\u2212<\/jats:sup>) mice were thus designed and used to test the hypothesis. Vessels, which normally show contraction to PGI<jats:sub>2<\/jats:sub>, were isolated for functional and biochemical analyses. Here, we showed that the contractile response evoked by PGI<jats:sub>2<\/jats:sub>was indeed only partially abolished in the abdominal aorta of TP<jats:sup>\u2212\/\u2212<\/jats:sup>mice. Interestingly, further antagonizing the E-type prostaglandin receptor EP3 removed the remaining contractile activity, resulting in relaxation evoked by PGI<jats:sub>2<\/jats:sub>in such vessels of TP<jats:sup>\u2212\/\u2212<\/jats:sup>mice. These results suggest that EP3 along with TP contributes to vasoconstrictor responses evoked by PGI<jats:sub>2<\/jats:sub>, and hence imply a novel mechanism for endothelial cyclooxygenase metabolites (which consist mainly of PGI<jats:sub>2<\/jats:sub>) in regulating vascular functions.<\/jats:p>","DOI":"10.1038\/srep42167","type":"journal-article","created":{"date-parts":[[2017,2,6]],"date-time":"2017-02-06T10:08:06Z","timestamp":1486375686000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Role of E-type prostaglandin receptor EP3 in the vasoconstrictor activity evoked by prostacyclin in thromboxane-prostanoid receptor deficient mice"],"prefix":"10.1038","volume":"7","author":[{"given":"Zhenhua","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingzhan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhong","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingbi","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,2,6]]},"reference":[{"key":"BFsrep42167_CR1","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1016\/0090-6980(76)90125-8","volume":"12","author":"S Bunting","year":"1976","unstructured":"Bunting, S., Gryglewski, R., Moncada, S. & Vane, J. 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