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This study investigates how age affects cartilage and subchondral bone changes in mouse joints following DMM. DMM was performed on male C57BL\/6 mice at 4 months (4\u2009M), 12 months (12\u2009M) and 19+ months (19\u2009M+) and on females at 12\u2009M and 18\u2009M+. Two months after surgery, operated and unoperated contralateral knees were harvested and evaluated using cartilage histology scores and \u03bcCT quantification of subchondral bone plate thickness and osteophyte formation. The 12\u2009M and 19\u2009M+ male mice developed more cartilage erosions and thicker subchondral bone plates after DMM than 4\u2009M males. The size of osteophytes trended up with age, while the bone volume fraction was significantly higher in the 19\u2009M+ group. Furthermore, 12\u2009M females developed milder OA than males as indicated by less cartilage degradation, less subchondral bone plate sclerosis and smaller osteophytes. Our results reveal distinct age\/gender-dependent structural changes in joint cartilage and subchondral bone post-DMM, facilitating more thoughtful selection of murine age\/gender when using this surgical technique for translational OA research.<\/jats:p>","DOI":"10.1038\/srep42294","type":"journal-article","created":{"date-parts":[[2017,2,9]],"date-time":"2017-02-09T10:25:10Z","timestamp":1486635910000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Age-dependent Changes in the Articular Cartilage and Subchondral Bone of C57BL\/6 Mice after Surgical Destabilization of Medial Meniscus"],"prefix":"10.1038","volume":"7","author":[{"given":"Henry","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordan D.","family":"Skelly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David C.","family":"Ayers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,2,9]]},"reference":[{"key":"BFsrep42294_CR1","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1159\/000213866","volume":"43","author":"AJ Bailey","year":"1997","unstructured":"Bailey, A. 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