{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:48:32Z","timestamp":1777510112572,"version":"3.51.4"},"reference-count":63,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,2,16]],"date-time":"2017-02-16T00:00:00Z","timestamp":1487203200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,2,16]],"date-time":"2017-02-16T00:00:00Z","timestamp":1487203200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Microcin PDI inhibits a diversity of pathogenic <jats:italic>Escherichia coli<\/jats:italic> through the action of an effector protein, McpM. In this study we demonstrated that expression of the inhibitory phenotype is induced under low osmolarity conditions and expression is primarily controlled by the EnvZ\/OmpR two-component regulatory system. Functional, mutagenesis and complementation experiments were used to empirically demonstrate that EnvZ is required for the inhibitory phenotype and that regulation of <jats:italic>mcpM<\/jats:italic> is dependent on binding of the phosphorylated OmpR to the <jats:italic>mcpM<\/jats:italic> promoter region. The phosphorylated OmpR may recognize three different binding sites within this promoter region. Site-directed mutagenesis revealed that the McpM precursor peptide includes two leader peptides that undergo sequential cleavage at positions G17\/G18 and G35\/A36 during export through the type I secretion system. Competition assays showed that both cleaved products are required for the PDI phenotype although we could not distinguish loss of function from loss of secretion in these assays. McpM has four cysteines within the mature peptide and site-directed mutagenesis experiments demonstrated that the first two cysteines are necessary for McpM to inhibit susceptible cells. Together these data combined with previous work indicate that MccPDI is unique amongst the microcins that have been described to date.<\/jats:p>","DOI":"10.1038\/srep42529","type":"journal-article","created":{"date-parts":[[2017,2,16]],"date-time":"2017-02-16T10:34:09Z","timestamp":1487241249000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Microcin PDI regulation and proteolytic cleavage are unique among known microcins"],"prefix":"10.1038","volume":"7","author":[{"given":"Zhe","family":"Zhao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa H.","family":"Orfe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinxin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shao-Yeh","family":"Lu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas E.","family":"Besser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas R.","family":"Call","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,2,16]]},"reference":[{"key":"BFsrep42529_CR1","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1038\/nrmicro2259","volume":"8","author":"ME Hibbing","year":"2010","unstructured":"Hibbing, M. E., Fuqua, C., Parsek, M. R. & Peterson, S. B. Bacterial competition: surviving and thriving in the microbial jungle. Nat Rev Microbiol 8, 15\u201325, doi: 10.1038\/nrmicro2259 (2010).","journal-title":"Nat Rev Microbiol"},{"key":"BFsrep42529_CR2","unstructured":"Riley, M. A. In Prokaryotic antimicrobial peptides: from genes to applications (eds Drider, D. & Rebuffat, S. ) Ch. 2, 13\u201326 (Springer, New York, New York, 2011)."},{"key":"BFsrep42529_CR3","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1146\/annurev.micro.56.012302.161024","volume":"56","author":"MA Riley","year":"2002","unstructured":"Riley, M. A. & Wertz, J. E. Bacteriocins: evolution, ecology, and application. Annu Rev Microbiol 56, 117\u2013137, doi: 10.1146\/annurev.micro.56.012302.161024 (2002).","journal-title":"Annu Rev Microbiol"},{"key":"BFsrep42529_CR4","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/S0006-291X(76)80264-1","volume":"69","author":"C Asensio","year":"1976","unstructured":"Asensio, C. & Perez-Diaz, J. C. A new family of low molecular weight antibiotics from enterobacteria. Biochem Biophys Res Commun 69, 7\u201314 (1976).","journal-title":"Biochem Biophys Res Commun"},{"key":"BFsrep42529_CR5","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1111\/j.1574-6968.1984.tb01046.x","volume":"23","author":"F Baquero","year":"1984","unstructured":"Baquero, F. & Moreno, F. The microcins. FEMS Microbiology Letters 23, 117\u2013124, doi: 10.1111\/j.1574-6968.1984.tb01046.x (1984).","journal-title":"FEMS Microbiology Letters"},{"key":"BFsrep42529_CR6","doi-asserted-by":"publisher","first-page":"708","DOI":"10.1039\/b516237h","volume":"24","author":"S Duquesne","year":"2007","unstructured":"Duquesne, S., Destoumieux-Garzon, D., Peduzzi, J. & Rebuffat, S. Microcins, gene-encoded antibacterial peptides from enterobacteria. Nat Prod Rep 24, 708\u2013734, doi: 10.1039\/b516237h (2007).","journal-title":"Nat Prod Rep"},{"key":"BFsrep42529_CR7","doi-asserted-by":"crossref","unstructured":"Rebuffat, S. In Prokaryotic Antimicrobial Peptides: from genes to applications (eds Djamel, Drider & Sylvie, Rebuffat ) Ch. 4, 55\u201372 (Springer: New York, 2011).","DOI":"10.1007\/978-1-4419-7692-5_4"},{"key":"BFsrep42529_CR8","doi-asserted-by":"publisher","first-page":"1380","DOI":"10.1111\/j.1365-2958.2007.05874.x","volume":"65","author":"K Severinov","year":"2007","unstructured":"Severinov, K., Semenova, E., Kazakov, A., Kazakov, T. & Gelfand, M. S. Low-molecular-weight post-translationally modified microcins. Mol Microbiol 65, 1380\u20131394, doi: 10.1111\/j.1365-2958.2007.05874.x (2007).","journal-title":"Mol Microbiol"},{"key":"BFsrep42529_CR9","doi-asserted-by":"crossref","unstructured":"Severinov, K., Semenova, E. & Kazakov, T. In Prokaryotic Antimicrobial Peptides: from Genes to Applications (eds Djamel, Drider & Sylvie, Rebuffat ) Ch. 15, 289\u2013308 (Springer: New York, 2011).","DOI":"10.1007\/978-1-4419-7692-5_15"},{"key":"BFsrep42529_CR10","doi-asserted-by":"crossref","unstructured":"Vassiliadis, G., Destoumieux-Garzon, D. & Peduzzi, J. In Prokaryotic Antimicrobial Peptides: from Genes to Applications (eds Djamel, Drider & Sylvie, Rebuffat ) Ch. 16, 309\u2013330 (Springer: New York, 2011).","DOI":"10.1007\/978-1-4419-7692-5_16"},{"key":"BFsrep42529_CR11","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1146\/annurev.mi.46.100192.001041","volume":"46","author":"R Kolter","year":"1992","unstructured":"Kolter, R. & Moreno, F. Genetics of ribosomally synthesized peptide antibiotics. Annu Rev Microbiol 46, 141\u2013163, doi: 10.1146\/annurev.mi.46.100192.001041 (1992).","journal-title":"Annu Rev Microbiol"},{"key":"BFsrep42529_CR12","doi-asserted-by":"publisher","first-page":"3875","DOI":"10.1002\/j.1460-2075.1990.tb07606.x","volume":"9","author":"L Gilson","year":"1990","unstructured":"Gilson, L., Mahanty, H. K. & Kolter, R. Genetic analysis of an MDR-like export system: the secretion of colicin V. Embo J 9, 3875\u20133884 (1990).","journal-title":"Embo J"},{"key":"BFsrep42529_CR13","doi-asserted-by":"publisher","first-page":"2383","DOI":"10.1099\/13500872-140-9-2383","volume":"140","author":"LS Havarstein","year":"1994","unstructured":"Havarstein, L. S., Holo, H. & Nes, I. F. The leader peptide of colicin V shares consensus sequences with leader peptides that are common among peptide bacteriocins produced by gram-positive bacteria. Microbiology 140 (Pt 9), 2383\u20132389, doi: 10.1099\/13500872-140-9-2383 (1994).","journal-title":"Microbiology"},{"key":"BFsrep42529_CR14","doi-asserted-by":"publisher","first-page":"28057","DOI":"10.1074\/jbc.271.45.28057","volume":"271","author":"X Zhong","year":"1996","unstructured":"Zhong, X., Kolter, R. & Tai, P. C. Processing of colicin V-1, a secretable marker protein of a bacterial ATP binding cassette export system, requires membrane integrity, energy, and cytosolic factors. J Biol Chem 271, 28057\u201328063 (1996).","journal-title":"J Biol Chem"},{"key":"BFsrep42529_CR15","doi-asserted-by":"publisher","first-page":"2345","DOI":"10.1128\/AEM.03150-09","volume":"77","author":"AA Sawant","year":"2011","unstructured":"Sawant, A. A., Casavant, N. C., Call, D. R. & Besser, T. E. Proximity-dependent inhibition in Escherichia coli isolates from cattle. Appl Environ Microbiol 77, 2345\u20132351, doi: 10.1128\/AEM.03150-09 (2011).","journal-title":"Appl Environ Microbiol"},{"issue":"18","key":"BFsrep42529_CR16","doi-asserted-by":"publisher","first-page":"6592","DOI":"10.1128\/AEM.01067-12","volume":"78","author":"Lauren J. Eberhart","year":"2012","unstructured":"Eberhart, L. J. et al. Characterization of a novel microcin that kills enterohemorrhagic Escherichia coli O157:H7 and O26. Appl Environ Microbiol 78, 6592\u20136599, doi: 10.1128\/AEM.01067-12.","journal-title":"Applied and Environmental Microbiology"},{"key":"BFsrep42529_CR17","doi-asserted-by":"publisher","first-page":"6953","DOI":"10.1128\/AEM.01704-15","volume":"81","author":"Z Zhao","year":"2015","unstructured":"Zhao, Z. et al. Genome-wide screening identifies six genes that are associated with susceptibility to Escherichia coli microcin PDI. Appl Environ Microbiol 81, 6953\u20136963, doi: 10.1128\/AEM.01704-15 (2015).","journal-title":"Appl Environ Microbiol"},{"key":"BFsrep42529_CR18","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1016\/0923-2508(94)90083-3","volume":"145","author":"SA Forst","year":"1994","unstructured":"Forst, S. A. & Roberts, D. L. Signal transduction by the EnvZ-OmpR phosphotransfer system in bacteria. Res Microbiol 145, 363\u2013373 (1994).","journal-title":"Res Microbiol"},{"key":"BFsrep42529_CR19","doi-asserted-by":"publisher","first-page":"24155","DOI":"10.1074\/jbc.M110715200","volume":"277","author":"SJ Cai","year":"2002","unstructured":"Cai, S. J. & Inouye, M. EnvZ-OmpR interaction and osmoregulation in Escherichia coli . J Biol Chem 277, 24155\u201324161, doi: 10.1074\/jbc.M110715200 (2002).","journal-title":"J Biol Chem"},{"key":"BFsrep42529_CR20","doi-asserted-by":"publisher","first-page":"6052","DOI":"10.1073\/pnas.86.16.6052","volume":"86","author":"S Forst","year":"1989","unstructured":"Forst, S., Delgado, J. & Inouye, M. Phosphorylation of OmpR by the osmosensor EnvZ modulates expression of the ompF and ompC genes in Escherichia coli . Proc Natl Acad Sci USA 86, 6052\u20136056 (1989).","journal-title":"Proc Natl Acad Sci USA"},{"key":"BFsrep42529_CR21","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1046\/j.1365-2958.2000.01837.x","volume":"36","author":"L Qin","year":"2000","unstructured":"Qin, L., Dutta, R., Kurokawa, H., Ikura, M. & Inouye, M. A monomeric histidine kinase derived from EnvZ, an Escherichia coli osmosensor. Mol Microbiol 36, 24\u201332 (2000).","journal-title":"Mol Microbiol"},{"key":"BFsrep42529_CR22","doi-asserted-by":"publisher","first-page":"1131","DOI":"10.1046\/j.1365-2958.2003.03502.x","volume":"48","author":"X Feng","year":"2003","unstructured":"Feng, X., Oropeza, R. & Kenney, L. J. Dual regulation by phospho-OmpR of ssrA\/B gene expression in Salmonella pathogenicity island 2. Mol Microbiol 48, 1131\u20131143 (2003).","journal-title":"Mol Microbiol"},{"key":"BFsrep42529_CR23","doi-asserted-by":"publisher","first-page":"2038","DOI":"10.1128\/JB.187.6.2038-2049.2005","volume":"187","author":"G Jubelin","year":"2005","unstructured":"Jubelin, G. et al. CpxR\/OmpR interplay regulates curli gene expression in response to osmolarity In Escherichia coli. J Bacteriol 187, 2038\u20132049, doi: 10.1128\/JB.187.6.2038-2049.2005 (2005).","journal-title":"Escherichia coli. J Bacteriol"},{"key":"BFsrep42529_CR24","doi-asserted-by":"publisher","first-page":"26849","DOI":"10.1074\/jbc.270.45.26849","volume":"270","author":"SL Harlocker","year":"1995","unstructured":"Harlocker, S. L., Bergstrom, L. & Inouye, M. Tandem binding of six OmpR proteins to the ompF upstream regulatory sequence of Escherichia coli . J Biol Chem 270, 26849\u201326856 (1995).","journal-title":"J Biol Chem"},{"key":"BFsrep42529_CR25","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1128\/AAC.48.2.505-513.2004","volume":"48","author":"AM Pons","year":"2004","unstructured":"Pons, A. M. et al. Genetic analysis and complete primary structure of microcin L. Antimicrob Agents Chemother 48, 505\u2013513 (2004).","journal-title":"Antimicrob Agents Chemother"},{"key":"BFsrep42529_CR26","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1093\/jb\/mvp102","volume":"146","author":"K Inaba","year":"2009","unstructured":"Inaba, K. Disulfide bond formation system in Escherichia coli . J Biochem 146, 591\u2013597, doi: 10.1093\/jb\/mvp102 (2009).","journal-title":"J Biochem"},{"key":"BFsrep42529_CR27","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1128\/MMBR.00016-05","volume":"70","author":"D Drider","year":"2006","unstructured":"Drider, D., Fimland, G., Hechard, Y., McMullen, L. M. & Prevost, H. The continuing story of class IIa bacteriocins. Microbiol Mol Biol Rev 70, 564\u2013582, doi: 10.1128\/MMBR.00016-05 (2006).","journal-title":"Microbiol Mol Biol Rev"},{"key":"BFsrep42529_CR28","doi-asserted-by":"publisher","first-page":"416","DOI":"10.1016\/S0968-0004(00)89091-X","volume":"20","author":"GC Walker","year":"1995","unstructured":"Walker, G. C. SOS-regulated proteins in translesion DNA synthesis and mutagenesis. Trends Biochem Sci 20, 416\u2013420 (1995).","journal-title":"Trends Biochem Sci"},{"key":"BFsrep42529_CR29","doi-asserted-by":"publisher","first-page":"1783","DOI":"10.1099\/mic.0.2007\/016139-0","volume":"154","author":"O Gillor","year":"2008","unstructured":"Gillor, O., Vriezen, J. A. & Riley, M. A. The role of SOS boxes in enteric bacteriocin regulation. Microbiology 154, 1783\u20131792, doi: 10.1099\/mic.0.2007\/016139-0 (2008).","journal-title":"Microbiology"},{"key":"BFsrep42529_CR30","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1016\/S0300-9084(02)01452-9","volume":"84","author":"F Moreno","year":"2002","unstructured":"Moreno, F., Gonzalez-Pastor, J. E., Baquero, M. R. & Bravo, D. The regulation of microcin B, C and J operons. Biochimie 84, 521\u2013529 (2002).","journal-title":"Biochimie"},{"key":"BFsrep42529_CR31","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1016\/S0300-9084(02)01415-3","volume":"84","author":"G Corsini","year":"2002","unstructured":"Corsini, G., Baeza, M., Monasterio, O. & Lagos, R. The expression of genes involved in microcin maturation regulates the production of active microcin E492. Biochimie 84, 539\u2013544 (2002).","journal-title":"Biochimie"},{"key":"BFsrep42529_CR32","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1111\/j.1365-2958.1987.tb00512.x","volume":"1","author":"N Connell","year":"1987","unstructured":"Connell, N., Han, Z., Moreno, F. & Kolter, R. An E. coli promoter induced by the cessation of growth. Mol Microbiol 1, 195\u2013201 (1987).","journal-title":"Mol Microbiol"},{"key":"BFsrep42529_CR33","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1128\/AEM.65.1.291-293.1999","volume":"65","author":"P Uguen","year":"1999","unstructured":"Uguen, P., Hamelin, J., Le Pennec, J. P. & Blanco, C. Influence of osmolarity and the presence of an osmoprotectant on Lactococcus lactis growth and bacteriocin production. Appl Environ Microbiol 65, 291\u2013293 (1999).","journal-title":"Appl Environ Microbiol"},{"key":"BFsrep42529_CR34","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1146\/annurev.biochem.69.1.183","volume":"69","author":"AM Stock","year":"2000","unstructured":"Stock, A. M., Robinson, V. L. & Goudreau, P. N. Two-component signal transduction. Annu Rev Biochem 69, 183\u2013215, doi: 10.1146\/annurev.biochem.69.1.183 (2000).","journal-title":"Annu Rev Biochem"},{"key":"BFsrep42529_CR35","doi-asserted-by":"publisher","first-page":"1058","DOI":"10.1128\/jb.167.3.1058-1065.1986","volume":"167","author":"C Hernandez-Chico","year":"1986","unstructured":"Hernandez-Chico, C., San Millan, J. L., Kolter, R. & Moreno, F. Growth phase and ompR regulation of transcription of microcin B17 genes. J Bacteriol 167, 1058\u20131065 (1986).","journal-title":"J Bacteriol"},{"key":"BFsrep42529_CR36","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.1046\/j.1365-2958.2002.03240.x","volume":"46","author":"T Yoshida","year":"2002","unstructured":"Yoshida, T., Cai, S. & Inouye, M. Interaction of EnvZ, a sensory histidine kinase, with phosphorylated OmpR, the cognate response regulator. Mol Microbiol 46, 1283\u20131294 (2002).","journal-title":"Mol Microbiol"},{"key":"BFsrep42529_CR37","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1002\/prot.340010305","volume":"1","author":"J Davagnino","year":"1986","unstructured":"Davagnino, J., Herrero, M., Furlong, D., Moreno, F. & Kolter, R. The DNA replication inhibitor microcin B17 is a forty-three-amino-acid protein containing sixty percent glycine. Proteins 1, 230\u2013238, doi: 10.1002\/prot.340010305 (1986).","journal-title":"Proteins"},{"key":"BFsrep42529_CR38","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1074\/mcp.M100001-MCP200","volume":"1","author":"DJ Brooks","year":"2002","unstructured":"Brooks, D. J. & Fresco, J. R. Increased frequency of cysteine, tyrosine, and phenylalanine residues since the last universal ancestor. Mol Cell Proteomics 1, 125\u2013131 (2002).","journal-title":"Mol Cell Proteomics"},{"key":"BFsrep42529_CR39","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0006-291X(02)02770-5","volume":"300","author":"NM Giles","year":"2003","unstructured":"Giles, N. M., Giles, G. I. & Jacob, C. Multiple roles of cysteine in biocatalysis. Biochem Biophys Res Commun 300, 1\u20134 (2003).","journal-title":"Biochem Biophys Res Commun"},{"key":"BFsrep42529_CR40","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1016\/j.pep.2011.10.009","volume":"82","author":"M Berkmen","year":"2012","unstructured":"Berkmen, M. Production of disulfide-bonded proteins in Escherichia coli . Protein Expr Purif 82, 240\u2013251, doi: 10.1016\/j.pep.2011.10.009 (2012).","journal-title":"Protein Expr Purif"},{"key":"BFsrep42529_CR41","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1111\/j.1432-1033.1995.414_b.x","volume":"234","author":"A Bayer","year":"1995","unstructured":"Bayer, A., Freund, S. & Jung, G. Post-translational heterocyclic backbone modifications in the 43-peptide antibiotic microcin B17. Structure elucidation and NMR study of a 13C,15N-labelled gyrase inhibitor. Eur J Biochem 234, 414\u2013426 (1995).","journal-title":"Eur J Biochem"},{"key":"BFsrep42529_CR42","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1093\/genetics\/141.1.25","volume":"141","author":"LH Zhang","year":"1995","unstructured":"Zhang, L. H., Fath, M. J., Mahanty, H. K., Tai, P. C. & Kolter, R. Genetic analysis of the colicin V secretion pathway. Genetics 141, 25\u201332 (1995).","journal-title":"Genetics"},{"key":"BFsrep42529_CR43","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1042\/BJ20042107","volume":"389","author":"D Destoumieux-Garzon","year":"2005","unstructured":"Destoumieux-Garzon, D. et al. The iron-siderophore transporter FhuA is the receptor for the antimicrobial peptide microcin J25: role of the microcin Val11-Pro16 beta-hairpin region in the recognition mechanism. Biochem J 389, 869\u2013876, doi: 10.1042\/BJ20042107 (2005).","journal-title":"Biochem J"},{"key":"BFsrep42529_CR44","doi-asserted-by":"publisher","first-page":"7741","DOI":"10.1128\/jb.175.23.7741-7742.1993","volume":"175","author":"RA Salomon","year":"1993","unstructured":"Salomon, R. A. & Farias, R. N. The FhuA protein is involved in microcin 25 uptake. J Bacteriol 175, 7741\u20137742 (1993).","journal-title":"J Bacteriol"},{"key":"BFsrep42529_CR45","doi-asserted-by":"publisher","first-page":"3323","DOI":"10.1128\/jb.177.11.3323-3325.1995","volume":"177","author":"RA Salomon","year":"1995","unstructured":"Salomon, R. A. & Farias, R. N. The peptide antibiotic microcin 25 is imported through the TonB pathway and the SbmA protein. J Bacteriol 177, 3323\u20133325 (1995).","journal-title":"J Bacteriol"},{"key":"BFsrep42529_CR46","doi-asserted-by":"publisher","first-page":"12475","DOI":"10.1021\/ja036756q","volume":"125","author":"KA Wilson","year":"2003","unstructured":"Wilson, K. A. et al. Structure of microcin J25, a peptide inhibitor of bacterial RNA polymerase, is a lassoed tail. J Am Chem Soc 125, 12475\u201312483, doi: 10.1021\/ja036756q (2003).","journal-title":"J Am Chem Soc"},{"key":"BFsrep42529_CR47","first-page":"1685","volume":"132","author":"M Lavina","year":"1986","unstructured":"Lavina, M., Pugsley, A. P. & Moreno, F. Identification, mapping, cloning and characterization of a gene (sbmA) required for microcin B17 action on Escherichia coli K12. J Gen Microbiol 132, 1685\u20131693 (1986).","journal-title":"J Gen Microbiol"},{"key":"BFsrep42529_CR48","doi-asserted-by":"publisher","first-page":"8361","DOI":"10.1128\/JB.01028-07","volume":"189","author":"M Novikova","year":"2007","unstructured":"Novikova, M. et al. The Escherichia coli Yej transporter is required for the uptake of translation inhibitor microcin C. J Bacteriol 189, 8361\u20138365, doi: 10.1128\/JB.01028-07 (2007).","journal-title":"J Bacteriol"},{"key":"BFsrep42529_CR49","doi-asserted-by":"publisher","first-page":"2607","DOI":"10.1128\/JB.01956-07","volume":"190","author":"T Kazakov","year":"2008","unstructured":"Kazakov, T. et al. Escherichia coli peptidase A, B, or N can process translation inhibitor microcin C. J Bacteriol 190, 2607\u20132610, doi: 10.1128\/JB.01956-07 (2008).","journal-title":"J Bacteriol"},{"key":"BFsrep42529_CR50","doi-asserted-by":"publisher","first-page":"18033","DOI":"10.1074\/jbc.M513174200","volume":"281","author":"A Metlitskaya","year":"2006","unstructured":"Metlitskaya, A. et al. Aspartyl-tRNA synthetase is the target of peptide nucleotide antibiotic Microcin C. J Biol Chem 281, 18033\u201318042, doi: 10.1074\/jbc.M513174200 (2006).","journal-title":"J Biol Chem"},{"key":"BFsrep42529_CR51","doi-asserted-by":"publisher","first-page":"28233","DOI":"10.1074\/jbc.M400228200","volume":"279","author":"X Thomas","year":"2004","unstructured":"Thomas, X. et al. Siderophore peptide, a new type of post-translationally modified antibacterial peptide with potent activity. J Biol Chem 279, 28233\u201328242, doi: 10.1074\/jbc.M400228200 (2004).","journal-title":"J Biol Chem"},{"key":"BFsrep42529_CR52","doi-asserted-by":"publisher","first-page":"2557","DOI":"10.1099\/mic.0.26396-0","volume":"149","author":"SI Patzer","year":"2003","unstructured":"Patzer, S. I., Baquero, M. R., Bravo, D., Moreno, F. & Hantke, K. The colicin G, H and X determinants encode microcins M and H47, which might utilize the catecholate siderophore receptors FepA, Cir, Fiu and IroN. Microbiology 149, 2557\u20132570 (2003).","journal-title":"Microbiology"},{"key":"BFsrep42529_CR53","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1007\/s10534-005-4452-9","volume":"19","author":"D Destoumieux-Garzon","year":"2006","unstructured":"Destoumieux-Garzon, D., Peduzzi, J., Thomas, X., Djediat, C. & Rebuffat, S. Parasitism of iron-siderophore receptors of Escherichia coli by the siderophore-peptide microcin E492m and its unmodified counterpart. Biometals 19, 181\u2013191, doi: 10.1007\/s10534-005-4452-9 (2006).","journal-title":"Biometals"},{"key":"BFsrep42529_CR54","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/0014-5793(93)80096-D","volume":"321","author":"R Lagos","year":"1993","unstructured":"Lagos, R., Wilkens, M., Vergara, C., Cecchi, X. & Monasterio, O. Microcin E492 forms ion channels in phospholipid bilayer membrane. FEBS Lett 321, 145\u2013148 (1993).","journal-title":"FEBS Lett"},{"key":"BFsrep42529_CR55","doi-asserted-by":"publisher","first-page":"1031","DOI":"10.1046\/j.1365-2958.2003.03610.x","volume":"49","author":"D Destoumieux-Garzon","year":"2003","unstructured":"Destoumieux-Garzon, D. et al. Microcin E492 antibacterial activity: evidence for a TonB-dependent inner membrane permeabilization on Escherichia coli . Mol Microbiol 49, 1031\u20131041 (2003).","journal-title":"Mol Microbiol"},{"key":"BFsrep42529_CR56","doi-asserted-by":"publisher","first-page":"7049","DOI":"10.1128\/JB.00688-06","volume":"188","author":"S Bieler","year":"2006","unstructured":"Bieler, S., Silva, F., Soto, C. & Belin, D. Bactericidal activity of both secreted and nonsecreted microcin E492 requires the mannose permease. J Bacteriol 188, 7049\u20137061, doi: 10.1128\/JB.00688-06 (2006).","journal-title":"J Bacteriol"},{"key":"BFsrep42529_CR57","doi-asserted-by":"publisher","first-page":"3128","DOI":"10.1128\/AAC.45.11.3128-3131.2001","volume":"45","author":"M Trujillo","year":"2001","unstructured":"Trujillo, M., Rodriguez, E. & Lavina, M. ATP synthase is necessary for microcin H47 antibiotic action. Antimicrob Agents Chemother 45, 3128\u20133131, doi: 10.1128\/AAC.45.11.3128-3131.2001 (2001).","journal-title":"Antimicrob Agents Chemother"},{"key":"BFsrep42529_CR58","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1128\/jb.158.2.757-759.1984","volume":"158","author":"CC Yang","year":"1984","unstructured":"Yang, C. C. & Konisky, J. Colicin V-treated Escherichia coli does not generate membrane potential. J Bacteriol 158, 757\u2013759 (1984).","journal-title":"J Bacteriol"},{"key":"BFsrep42529_CR59","doi-asserted-by":"publisher","first-page":"1945","DOI":"10.1128\/JB.187.6.1945-1950.2005","volume":"187","author":"F Gerard","year":"2005","unstructured":"Gerard, F., Pradel, N. & Wu, L. F. Bactericidal activity of colicin V is mediated by an inner membrane protein, SdaC, of Escherichia coli. J Bacteriol 187, 1945\u20131950, doi: 10.1128\/JB.187.6.1945-1950.2005 (2005).","journal-title":"J Bacteriol"},{"key":"BFsrep42529_CR60","doi-asserted-by":"publisher","first-page":"924","DOI":"10.1038\/nprot.2007.132","volume":"2","author":"KL Heckman","year":"2007","unstructured":"Heckman, K. L. & Pease, L. R. Gene splicing and mutagenesis by PCR-driven overlap extension. Nat Protoc 2, 924\u2013932, doi: 10.1038\/nprot.2007.132 (2007).","journal-title":"Nat Protoc"},{"key":"BFsrep42529_CR61","doi-asserted-by":"publisher","first-page":"1310","DOI":"10.1128\/jb.178.5.1310-1319.1996","volume":"178","author":"DL Milton","year":"1996","unstructured":"Milton, D. L., O\u2019Toole, R., Horstedt, P. & Wolf-Watz, H. Flagellin A is essential for the virulence of Vibrio anguillarum . J Bacteriol 178, 1310\u20131319 (1996).","journal-title":"J Bacteriol"},{"key":"BFsrep42529_CR62","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1016\/S0167-7012(03)00194-5","volume":"55","author":"CY Chen","year":"2003","unstructured":"Chen, C. Y., Nace, G. W. & Irwin, P. L. A 6\u2009\u00d7\u20096 drop plate method for simultaneous colony counting and MPN enumeration of Campylobacter jejuni, Listeria monocytogenes, and Escherichia coli . J Microbiol Methods 55, 475\u2013479 (2003).","journal-title":"J Microbiol Methods"},{"key":"BFsrep42529_CR63","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak, K. J. & Schmittgen, T. D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25, 402\u2013408, doi: 10.1006\/meth.2001.1262 (2001).","journal-title":"Methods"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep42529.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep42529","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep42529.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,24]],"date-time":"2022-12-24T01:35:24Z","timestamp":1671845724000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep42529"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,16]]},"references-count":63,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,3,14]]}},"alternative-id":["BFsrep42529"],"URL":"https:\/\/doi.org\/10.1038\/srep42529","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,16]]},"assertion":[{"value":"28 September 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 January 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 February 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"42529"}}