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Our study revealed that a total of 6, 5, 2 and 10 bacterial genera showed biologically significant differences in the GITs of pre-weaned calves fed four waste-milk diets: acidified waste milk, pasteurised waste milk, untreated bulk milk, and untreated waste milk, respectively. Specifically, generic biomarkers were observed in the rumen (e.g.,<jats:italic>Bifidobacterium, Parabacteroides, Fibrobacter, Clostridium<\/jats:italic>, etc.), caecum (e.g.,<jats:italic>Faecalibacterium, Oxalobacter, Odoribacter<\/jats:italic>, etc.) and colon (e.g.,<jats:italic>Megamonas, Comamonas, Stenotrophomonas<\/jats:italic>, etc.) but not in the faeces. In addition, the predicted metabolic pathways showed that the expression of genes related to metabolic diseases was increased in the calves fed untreated waste milk, which indicated that untreated waste milk is not a suitable liquid diet for pre-weaned calves. This is the first study to demonstrate how different types of waste milk fed to pre-weaned calves affect the community structure of colonised bacteria, and the results may provide insights for the intentional adjustment of diets and gastrointestinal bacterial communities.<\/jats:p>","DOI":"10.1038\/srep42689","type":"journal-article","created":{"date-parts":[[2017,3,10]],"date-time":"2017-03-10T10:09:20Z","timestamp":1489140560000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Influence of dairy by-product waste milk on the microbiomes of different gastrointestinal tract components in pre-weaned dairy calves"],"prefix":"10.1038","volume":"7","author":[{"given":"Y. F.","family":"Deng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. 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