{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T04:14:39Z","timestamp":1768709679008,"version":"3.49.0"},"reference-count":50,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,2,23]],"date-time":"2017-02-23T00:00:00Z","timestamp":1487808000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,2,23]],"date-time":"2017-02-23T00:00:00Z","timestamp":1487808000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Salusin-\u03b2 is a bioactive peptide involved in vascular smooth muscle cell proliferation, vascular fibrosis and hypertension. The present study was designed to determine the effects of silencing salusin-\u03b2 on hypertension and cardiovascular remodeling in spontaneously hypertensive rats (SHR). Thirteen-week-old male SHR and normotensive Wistar-Kyoto rats (WKY) were subjected to intravenous injection of PBS, adenoviral vectors encoding salusin-\u03b2 shRNA (Ad-Sal-shRNA) or a scramble shRNA. Salusin-\u03b2 levels in plasma, myocardium and mesenteric artery were increased in SHR. Silencing salusin-\u03b2 had no significant effect on blood pressure in WKY, but reduced blood pressure in SHR. It reduced the ratio of left ventricle weight to body weight, cross-sectional areas of cardiocytes and perivascular fibrosis, and decreased the media thickness and the media\/lumen ratio of arteries in SHR. Silencing salusin-\u03b2 almost normalized plasma norepinephrine and angiotensin II levels in SHR. It prevented the upregulation of angiotensin II and AT<jats:sub>1<\/jats:sub> receptors, and reduced the NAD(P)H oxidase activity and superoxide anion levels in myocardium and mesenteric artery of SHR. Knockdown of salusin-\u03b2 attenuated cell proliferation and fibrosis in vascular smooth muscle cells from SHR. These results indicate that silencing salusin-\u03b2 attenuates hypertension and cardiovascular remodeling in SHR.<\/jats:p>","DOI":"10.1038\/srep43259","type":"journal-article","created":{"date-parts":[[2017,2,23]],"date-time":"2017-02-23T10:37:42Z","timestamp":1487846262000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Silencing salusin-\u03b2 attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats"],"prefix":"10.1038","volume":"7","author":[{"given":"Xing-Sheng","family":"Ren","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Ling","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye-Bo","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue-Hua","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Ming","family":"Kang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guo-Qing","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,2,23]]},"reference":[{"key":"BFsrep43259_CR1","doi-asserted-by":"crossref","first-page":"1166","DOI":"10.1038\/nm913","volume":"9","author":"M Shichiri","year":"2003","unstructured":"Shichiri, M. et al. Salusins: newly identified bioactive peptides with hemodynamic and mitogenic activities. Nat. Med. 9, 1166\u20131172 (2003).","journal-title":"Nat. Med"},{"key":"BFsrep43259_CR2","doi-asserted-by":"crossref","first-page":"1255","DOI":"10.1291\/hypres.30.1255","volume":"30","author":"N Suzuki","year":"2007","unstructured":"Suzuki, N. et al. Systemic distribution of salusin expression in the rat. Hypertens. Res. 30, 1255\u20131262 (2007).","journal-title":"Hypertens. Res."},{"key":"BFsrep43259_CR3","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/j.peptides.2010.12.012","volume":"32","author":"N Suzuki","year":"2011","unstructured":"Suzuki, N., Shichiri, M., Tateno, T., Sato, K. & Hirata, Y. Distinct systemic distribution of salusin-alpha and salusin-beta in the rat. Peptides 32, 805\u2013810 (2011).","journal-title":"Peptides"},{"key":"BFsrep43259_CR4","doi-asserted-by":"crossref","first-page":"e76714","DOI":"10.1371\/journal.pone.0076714","volume":"8","author":"K Fujimoto","year":"2013","unstructured":"Fujimoto, K. et al. Circulating levels of human salusin-beta, a potent hemodynamic and atherogenesis regulator. PLoS. One. 8, e76714 (2013).","journal-title":"PLoS. One."},{"key":"BFsrep43259_CR5","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1097\/HJH.0000000000000156","volume":"32","author":"K Matsumura","year":"2014","unstructured":"Matsumura, K. Salusin and central regulation of blood pressure in hypertension. J. Hypertens. 32, 981\u2013982 (2014).","journal-title":"J. Hypertens."},{"key":"BFsrep43259_CR6","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1093\/cvr\/cvt031","volume":"98","author":"WW Chen","year":"2013","unstructured":"Chen, W. W. et al. Salusin-\u03b2 in paraventricular nucleus increases blood pressure and sympathetic outflow via vasopressin in hypertensive rats. Cardiovasc Res 98, 344\u2013351 (2013).","journal-title":"Cardiovasc Res"},{"key":"BFsrep43259_CR7","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1111\/apha.12188","volume":"210","author":"HJ Sun","year":"2014","unstructured":"Sun, H. J. et al. Superoxide anions involved in sympathoexcitation and pressor effects of salusin-beta in paraventricular nucleus in hypertensive rats. Acta Physiol (Oxf) 210, 534\u2013545 (2014).","journal-title":"Acta Physiol (Oxf)"},{"key":"BFsrep43259_CR8","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1097\/HJH.0000000000000143","volume":"32","author":"LL Zhang","year":"2014","unstructured":"Zhang, L. L. et al. Salusin-beta in rostral ventrolateral medulla increases sympathetic outflow and blood pressure via superoxide anions in hypertensive rats. J. Hypertens. 32, 1059\u20131067 (2014).","journal-title":"J. Hypertens."},{"key":"BFsrep43259_CR9","doi-asserted-by":"crossref","first-page":"11162","DOI":"10.1038\/srep11162","volume":"5","author":"HB Li","year":"2015","unstructured":"Li, H. B. et al. Central blockade of salusin beta attenuates hypertension and hypothalamic inflammation in spontaneously hypertensive rats. Sci. Rep. 5, 11162 (2015).","journal-title":"Sci. Rep"},{"key":"BFsrep43259_CR10","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1016\/j.bbadis.2015.05.008","volume":"1852","author":"HJ Sun","year":"2015","unstructured":"Sun, H. J. et al. Salusin-beta contributes to vascular remodeling associated with hypertension via promoting vascular smooth muscle cell proliferation and vascular fibrosis. Biochim. Biophys. Acta 1852, 1709\u20131718 (2015).","journal-title":"Biochim. Biophys. Acta"},{"key":"BFsrep43259_CR11","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/s11906-012-0325-0","volume":"15","author":"M Briet","year":"2013","unstructured":"Briet, M. & Schiffrin, E. L. Treatment of arterial remodeling in essential hypertension. Curr. Hypertens. Rep. 15, 3\u20139 (2013).","journal-title":"Curr. Hypertens. Rep."},{"key":"BFsrep43259_CR12","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1007\/s11906-006-0046-3","volume":"8","author":"D Rizzoni","year":"2006","unstructured":"Rizzoni, D. & Agabiti, R. E. Small artery remodeling in hypertension and diabetes. Curr. Hypertens. Rep. 8, 90\u201395 (2006).","journal-title":"Curr. Hypertens. Rep."},{"key":"BFsrep43259_CR13","first-page":"105","volume":"17","author":"MJ Mulvany","year":"2002","unstructured":"Mulvany, M. J. Small artery remodeling and significance in the development of hypertension. News Physiol Sci. 17, 105\u2013109 (2002).","journal-title":"News Physiol Sci"},{"key":"BFsrep43259_CR14","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1161\/HYPERTENSIONAHA.114.03449","volume":"64","author":"GF Mitchell","year":"2014","unstructured":"Mitchell, G. F. Arterial stiffness and hypertension: chicken or egg? Hypertension 64, 210\u2013214 (2014).","journal-title":"Hypertension"},{"key":"BFsrep43259_CR15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/jhh.2014.36","volume":"29","author":"W Nadruz","year":"2015","unstructured":"Nadruz, W. Myocardial remodeling in hypertension. J. Hum. Hypertens. 29, 1\u20136 (2015).","journal-title":"J. Hum. Hypertens."},{"key":"BFsrep43259_CR16","doi-asserted-by":"crossref","first-page":"23596","DOI":"10.1038\/srep23596","volume":"6","author":"HJ Sun","year":"2016","unstructured":"Sun, H. J. et al. Salusin-beta induces foam cell formation and monocyte adhesion in human vascular smooth muscle cells via miR155\/NOX2\/NFkappaB pathway. Sci. Rep. 6, 23596 (2016).","journal-title":"Sci. Rep"},{"key":"BFsrep43259_CR17","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1089\/ars.2015.6475","volume":"24","author":"HJ Sun","year":"2016","unstructured":"Sun, H. J. et al. Salusin-beta Promotes Vascular Smooth Muscle Cell migration and Intimal Hyperplasia after Vascular Injury via ROS\/NFkappaB\/MMP-9 Pathway. Antioxid. Redox. Signal. 24, 1045\u20131057 (2016).","journal-title":"Antioxid. Redox. Signal."},{"key":"BFsrep43259_CR18","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/S1054-8807(03)00135-2","volume":"13","author":"D Bell","year":"2004","unstructured":"Bell, D. et al. Temporal characteristics of cardiomyocyte hypertrophy in the spontaneously hypertensive rat. Cardiovasc. Pathol. 13, 71\u201378 (2004).","journal-title":"Cardiovasc. Pathol."},{"key":"BFsrep43259_CR19","first-page":"3","volume":"108","author":"D Graham","year":"2005","unstructured":"Graham, D., McBride, M. W., Brain, N. J. & Dominiczak, A. F. Congenic\/consomic models of hypertension. Methods Mol. Med. 108, 3\u201315 (2005).","journal-title":"Methods Mol. Med."},{"key":"BFsrep43259_CR20","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1111\/apha.12447","volume":"213","author":"WW Chen","year":"2015","unstructured":"Chen, W. W. et al. Cardiac sympathetic afferent reflex and its implications for sympathetic activation in chronic heart failure and hypertension. Acta Physiol (Oxf) 213, 778\u2013794 (2015).","journal-title":"Acta Physiol (Oxf)"},{"key":"BFsrep43259_CR21","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.jash.2016.02.015","volume":"10","author":"G Grassi","year":"2016","unstructured":"Grassi, G. & Ram, V. S. Evidence for a critical role of the sympathetic nervous system in hypertension. J. Am. Soc. Hypertens. 10, 457\u2013466 (2016).","journal-title":"J. Am. Soc. Hypertens"},{"key":"BFsrep43259_CR22","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1161\/HYPERTENSIONAHA.111.00633","volume":"61","author":"GF DiBona","year":"2013","unstructured":"DiBona, G. F. Sympathetic nervous system and hypertension. Hypertension 61, 556\u2013560 (2013).","journal-title":"Hypertension"},{"key":"BFsrep43259_CR23","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1161\/01.HYP.5.4.552","volume":"5","author":"DS Goldstein","year":"1983","unstructured":"Goldstein, D. S., McCarty, R., Polinsky, R. J. & Kopin, I. J. Relationship between plasma norepinephrine and sympathetic neural activity. Hypertension 5, 552\u2013559 (1983).","journal-title":"Hypertension"},{"key":"BFsrep43259_CR24","doi-asserted-by":"crossref","first-page":"R264","DOI":"10.1152\/ajpregu.00687.2009","volume":"300","author":"K Burgi","year":"2011","unstructured":"Burgi, K. et al. Tyrosine hydroxylase immunoreactivity as indicator of sympathetic activity: simultaneous evaluation in different tissues of hypertensive rats. Am. J. Physiol Regul. Integr. Comp Physiol 300, R264\u2013R271 (2011).","journal-title":"Am. J. Physiol Regul. Integr. Comp Physiol"},{"key":"BFsrep43259_CR25","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1007\/s11906-003-0073-2","volume":"5","author":"RM Touyz","year":"2003","unstructured":"Touyz, R. M. The role of angiotensin II in regulating vascular structural and functional changes in hypertension. Curr. Hypertens. Rep. 5, 155\u2013164 (2003).","journal-title":"Curr. Hypertens. Rep."},{"key":"BFsrep43259_CR26","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1161\/CIRCRESAHA.116.303587","volume":"116","author":"RL Te","year":"2015","unstructured":"Te, R. L., van Esch, J. H., Roks, A. J., van den Meiracker, A. H. & Danser, A. H. Hypertension: renin-angiotensin-aldosterone system alterations. Circ. Res. 116, 960\u2013975 (2015).","journal-title":"Circ. Res."},{"key":"BFsrep43259_CR27","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1056\/NEJMe020054","volume":"346","author":"JR Sowers","year":"2002","unstructured":"Sowers, J. R. Hypertension, angiotensin II, and oxidative stress. N. Engl. J. Med. 346, 1999\u20132001 (2002).","journal-title":"N. Engl. J. Med."},{"key":"BFsrep43259_CR28","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1046\/j.1440-1681.2003.03930.x","volume":"30","author":"RM Touyz","year":"2003","unstructured":"Touyz, R. M., Tabet, F. & Schiffrin, E. L. Redox-dependent signalling by angiotensin II and vascular remodelling in hypertension. Clin. Exp. Pharmacol. Physiol 30, 860\u2013866 (2003).","journal-title":"Clin. Exp. Pharmacol. Physiol"},{"key":"BFsrep43259_CR29","first-page":"I26","volume":"75","author":"DD Savage","year":"1987","unstructured":"Savage, D. D. et al. The spectrum of left ventricular hypertrophy in a general population sample: the Framingham Study. Circulation 75, I26\u2013I33 (1987).","journal-title":"Circulation"},{"key":"BFsrep43259_CR30","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1161\/01.HYP.11.2.198","volume":"11","author":"GK Owens","year":"1988","unstructured":"Owens, G. K., Schwartz, S. M. & McCanna, M. Evaluation of medial hypertrophy in resistance vessels of spontaneously hypertensive rats. Hypertension 11, 198\u2013207 (1988).","journal-title":"Hypertension"},{"key":"BFsrep43259_CR31","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1161\/hy09t1.096249","volume":"38","author":"HD Intengan","year":"2001","unstructured":"Intengan, H. D. & Schiffrin, E. L. Vascular remodeling in hypertension: roles of apoptosis, inflammation, and fibrosis. Hypertension 38, 581\u2013587 (2001).","journal-title":"Hypertension"},{"key":"BFsrep43259_CR32","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1016\/j.lfs.2016.01.022","volume":"146","author":"WY Kwon","year":"2016","unstructured":"Kwon, W. Y. et al. Interleukin-10 deficiency aggravates angiotensin II-induced cardiac remodeling in mice. Life Sci. 146, 214\u2013221 (2016).","journal-title":"Life Sci."},{"key":"BFsrep43259_CR33","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1161\/HYPERTENSIONAHA.115.06737","volume":"67","author":"SP Didion","year":"2016","unstructured":"Didion, S. P. New Insights Into Mechanisms Associated With Angiotensin II-Induced Vascular Hypertrophy and Remodeling. Hypertension 67, 501\u2013503 (2016).","journal-title":"Hypertension"},{"key":"BFsrep43259_CR34","doi-asserted-by":"crossref","first-page":"21692","DOI":"10.1038\/srep21692","volume":"6","author":"Y Deng","year":"2016","unstructured":"Deng, Y. et al. Prenatal inflammation-induced NF-kappaB dyshomeostasis contributes to renin-angiotensin system over-activity resulting in prenatally programmed hypertension in offspring. Sci. Rep. 6, 21692 (2016).","journal-title":"Sci. Rep"},{"key":"BFsrep43259_CR35","doi-asserted-by":"crossref","first-page":"797","DOI":"10.3390\/ijms17070797","volume":"17","author":"N Munoz-Durango","year":"2016","unstructured":"Munoz-Durango, N. et al. Role of the Renin-Angiotensin-Aldosterone System beyond Blood Pressure Regulation: Molecular and Cellular Mechanisms Involved in End-Organ Damage during Arterial Hypertension. Int. J. Mol. Sci. 17, 797 (2016).","journal-title":"Int. J. Mol. Sci."},{"key":"BFsrep43259_CR36","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1161\/CIRCRESAHA.116.303587","volume":"116","author":"RL Te","year":"2015","unstructured":"Te, R. L., van Esch, J. H., Roks, A. J., van den Meiracker, A. H. & Danser, A. H. Hypertension: renin-angiotensin-aldosterone system alterations. Circ. Res. 116, 960\u2013975 (2015).","journal-title":"Circ. Res."},{"key":"BFsrep43259_CR37","doi-asserted-by":"crossref","first-page":"132","DOI":"10.2174\/1573402111666150529130922","volume":"11","author":"N Sinha","year":"2015","unstructured":"Sinha, N. & Dabla, P. K. Oxidative stress and antioxidants in hypertension-a current review. Curr. Hypertens. Rev. 11, 132\u2013142 (2015).","journal-title":"Curr. Hypertens. Rev"},{"key":"BFsrep43259_CR38","doi-asserted-by":"crossref","first-page":"25342","DOI":"10.1038\/srep25342","volume":"6","author":"PW Cheng","year":"2016","unstructured":"Cheng, P. W. et al. Resveratrol Inhibition of Rac1-Derived Reactive Oxygen Species by AMPK Decreases Blood Pressure in a Fructose-Induced Rat Model of Hypertension. Sci. Rep. 6, 25342 (2016).","journal-title":"Sci. Rep"},{"key":"BFsrep43259_CR39","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1016\/j.cjca.2015.02.008","volume":"31","author":"AC Montezano","year":"2015","unstructured":"Montezano, A. C. et al. Oxidative stress and human hypertension: vascular mechanisms, biomarkers, and novel therapies. Can. J. Cardiol. 31, 631\u2013641 (2015).","journal-title":"Can. J. Cardiol."},{"key":"BFsrep43259_CR40","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1055\/s-0035-1548765","volume":"123","author":"R Brito","year":"2015","unstructured":"Brito, R., Castillo, G., Gonzalez, J., Valls, N. & Rodrigo, R. Oxidative stress in hypertension: mechanisms and therapeutic opportunities. Exp. Clin. Endocrinol. Diabetes 123, 325\u2013335 (2015).","journal-title":"Exp. Clin. Endocrinol. Diabetes"},{"key":"BFsrep43259_CR41","doi-asserted-by":"crossref","first-page":"1804","DOI":"10.1161\/CIRCRESAHA.114.302524","volume":"114","author":"G Mancia","year":"2014","unstructured":"Mancia, G. & Grassi, G. The autonomic nervous system and hypertension. Circ. Res. 114, 1804\u20131814 (2014).","journal-title":"Circ. Res."},{"key":"BFsrep43259_CR42","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1161\/01.HYP.34.4.724","volume":"34","author":"G Mancia","year":"1999","unstructured":"Mancia, G., Grassi, G., Giannattasio, C. & Seravalle, G. Sympathetic activation in the pathogenesis of hypertension and progression of organ damage. Hypertension 34, 724\u2013728 (1999).","journal-title":"Hypertension"},{"key":"BFsrep43259_CR43","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/j.brainres.2005.07.055","volume":"1058","author":"Y Han","year":"2005","unstructured":"Han, Y. et al. Reactive oxygen species in paraventricular nucleus modulates cardiac sympathetic afferent reflex in rats. Brain Res. 1058, 82\u201390 (2005).","journal-title":"Brain Res."},{"key":"BFsrep43259_CR44","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1016\/j.ejheart.2007.07.004","volume":"9","author":"Y Han","year":"2007","unstructured":"Han, Y. et al. Reactive oxygen species in the paraventricular nucleus mediate the cardiac sympathetic afferent reflex in chronic heart failure rats. Eur. J Heart Fail. 9, 967\u2013973 (2007).","journal-title":"Eur. J Heart Fail."},{"key":"BFsrep43259_CR45","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1093\/cvr\/cvp099","volume":"83","author":"S Gao","year":"2009","unstructured":"Gao, S., Long, C. L., Wang, R. H. & Wang & H. K(ATP) activation prevents progression of cardiac hypertrophy to failure induced by pressure overload via protecting endothelial function. Cardiovasc. Res. 83, 444\u2013456 (2009).","journal-title":"Cardiovasc. Res."},{"key":"BFsrep43259_CR46","doi-asserted-by":"crossref","first-page":"H51","DOI":"10.1152\/ajpheart.00240.2012","volume":"304","author":"Y Wang","year":"2013","unstructured":"Wang, Y. et al. Relaxant effect of all-trans-retinoic acid via NO-sGC-cGMP pathway and calcium-activated potassium channels in rat mesenteric artery. Am. J. Physiol Heart Circ. Physiol 304, H51\u2013H57 (2013).","journal-title":"Am. J. Physiol Heart Circ. Physiol"},{"key":"BFsrep43259_CR47","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jss.2005.07.029","volume":"131","author":"SG Houghton","year":"2006","unstructured":"Houghton, S. G., Nicholson, V. D. & Sarr, M. G. In vivo complete neural isolation of the rat jejunoileum: a simple model to study denervation sequelae of intestinal transplantation. J. Surg. Res. 131, 53\u201357 (2006).","journal-title":"J. Surg. Res."},{"key":"BFsrep43259_CR48","doi-asserted-by":"crossref","first-page":"H2093","DOI":"10.1152\/ajpheart.01045.2009","volume":"298","author":"H Raina","year":"2010","unstructured":"Raina, H., Zhang, Q., Rhee, A. Y., Pallone, T. L. & Wier, W. G. Sympathetic nerves and the endothelium influence the vasoconstrictor effect of low concentrations of ouabain in pressurized small arteries. Am. J. Physiol Heart Circ. Physiol 298, H2093\u2013H2101 (2010).","journal-title":"Am. J. Physiol Heart Circ. Physiol"},{"key":"BFsrep43259_CR49","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1093\/cvr\/cvq081","volume":"87","author":"T Bagnost","year":"2010","unstructured":"Bagnost, T. et al. Cardiovascular effects of arginase inhibition in spontaneously hypertensive rats with fully developed hypertension. Cardiovasc. Res. 87, 569\u2013577 (2010).","journal-title":"Cardiovasc. Res."},{"key":"BFsrep43259_CR50","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.atherosclerosis.2013.01.030","volume":"228","author":"EH Alcantara","year":"2013","unstructured":"Alcantara, E. H. et al. Long-term zinc deprivation accelerates rat vascular smooth muscle cell proliferation involving the down-regulation of JNK1\/2 expression in MAPK signaling. Atherosclerosis 228, 46\u201352 (2013).","journal-title":"Atherosclerosis"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep43259","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep43259.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep43259.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,23]],"date-time":"2022-12-23T19:37:10Z","timestamp":1671824230000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep43259"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,23]]},"references-count":50,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,4,10]]}},"alternative-id":["BFsrep43259"],"URL":"https:\/\/doi.org\/10.1038\/srep43259","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,23]]},"assertion":[{"value":"9 September 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 January 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 February 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"43259"}}