{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T19:33:46Z","timestamp":1770838426383,"version":"3.50.1"},"reference-count":86,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,3,6]],"date-time":"2017-03-06T00:00:00Z","timestamp":1488758400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,3,6]],"date-time":"2017-03-06T00:00:00Z","timestamp":1488758400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Retinal ischemia occurs in a variety of eye diseases. Restrained blood flow induces retinal damage, which leads to progressive optic nerve degeneration and vision loss. Previous studies indicate that extracellular matrix (ECM) constituents play an important role in complex tissues, such as retina and optic nerve. They have great impact on de- and regeneration processes and represent major candidates of central nervous system glial scar formation. Nevertheless, the importance of the ECM during ischemic retina and optic nerve neurodegeneration is not fully understood yet. In this study, we analyzed remodeling of the extracellular glycoproteins fibronectin, laminin, tenascin-C and tenascin-R and the chondroitin sulfate proteoglycans (CSPGs) aggrecan, brevican and phosphacan\/RPTP\u03b2\/\u03b6 in retinae and optic nerves of an ischemia\/reperfusion rat model via quantitative real-time PCR, immunohistochemistry and Western blot. A variety of ECM constituents were dysregulated in the retina and optic nerve after ischemia. Regarding fibronectin, significantly elevated mRNA and protein levels were observed in the retina following ischemia, while laminin and tenascin-C showed enhanced immunoreactivity in the optic nerve after ischemia. Interestingly, CSPGs displayed significantly increased expression levels in the optic nerve. Our study demonstrates a dynamic expression of ECM molecules following retinal ischemia, which strengthens their regulatory role during neurodegeneration.<\/jats:p>","DOI":"10.1038\/srep43470","type":"journal-article","created":{"date-parts":[[2017,3,6]],"date-time":"2017-03-06T11:16:10Z","timestamp":1488798970000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Ischemic injury leads to extracellular matrix alterations in retina and optic nerve"],"prefix":"10.1038","volume":"7","author":[{"given":"Jacqueline","family":"Reinhard","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Renner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Wiemann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel A.","family":"Shakoor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gesa","family":"Stute","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. Burkhard","family":"Dick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Faissner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie C.","family":"Joachim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,3,6]]},"reference":[{"key":"BFsrep43470_CR1","doi-asserted-by":"publisher","first-page":"2353","DOI":"10.1167\/iovs.12-11103","volume":"54","author":"DA Sim","year":"2013","unstructured":"Sim, D. A. et al. The effects of macular ischemia on visual acuity in diabetic retinopathy. Invest Ophthalmol Vis Sci 54, 2353\u20132360 (2013).","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"BFsrep43470_CR2","doi-asserted-by":"publisher","first-page":"1020","DOI":"10.1136\/bjophthalmol-2013-303143","volume":"97","author":"DJ Coleman","year":"2013","unstructured":"Coleman, D. J. et al. Age-related macular degeneration: choroidal ischaemia? Br J Ophthalmol 97, 1020\u20131023 (2013).","journal-title":"Br J Ophthalmol"},{"key":"BFsrep43470_CR3","doi-asserted-by":"publisher","first-page":"859","DOI":"10.1016\/S0161-6420(97)30221-8","volume":"104","author":"JB Mizener","year":"1997","unstructured":"Mizener, J. B., Podhajsky, P. & Hayreh, S. S. Ocular ischemic syndrome. Ophthalmology 104, 859\u2013864 (1997).","journal-title":"Ophthalmology"},{"key":"BFsrep43470_CR4","doi-asserted-by":"publisher","first-page":"1391","DOI":"10.1038\/nm.2507","volume":"17","author":"HK Eltzschig","year":"2011","unstructured":"Eltzschig, H. K. & Eckle, T. Ischemia and reperfusion\u2013from mechanism to translation. Nat Med 17, 1391\u20131401 (2011).","journal-title":"Nat Med"},{"key":"BFsrep43470_CR5","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1186\/s12863-015-0201-4","volume":"16","author":"K Andreeva","year":"2015","unstructured":"Andreeva, K., Soliman, M. M. & Cooper, N. G. Regulatory networks in retinal ischemia-reperfusion injury. BMC Genet 16, 43 (2015).","journal-title":"BMC Genet"},{"key":"BFsrep43470_CR6","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1097\/ICU.0000000000000043","volume":"25","author":"ND Chinskey","year":"2014","unstructured":"Chinskey, N. D., Besirli, C. G. & Zacks, D. N. Retinal cell death and current strategies in retinal neuroprotection. Curr Opin Ophthalmol 25, 228\u2013233 (2014).","journal-title":"Curr Opin Ophthalmol"},{"key":"BFsrep43470_CR7","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1186\/1750-1326-8-21","volume":"8","author":"BJ Kim","year":"2013","unstructured":"Kim, B. J., Braun, T. A., Wordinger, R. J. & Clark, A. F. Progressive morphological changes and impaired retinal function associated with temporal regulation of gene expression after retinal ischemia\/reperfusion injury in mice. Mol Neurodegener 8, 21 (2013).","journal-title":"Mol Neurodegener"},{"key":"BFsrep43470_CR8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.exer.2014.04.009","volume":"123","author":"G Dvoriantchikova","year":"2014","unstructured":"Dvoriantchikova, G., Degterev, A. & Ivanov, D. Retinal ganglion cell (RGC) programmed necrosis contributes to ischemia-reperfusion-induced retinal damage. Exp Eye Res 123, 1\u20137 (2014).","journal-title":"Exp Eye Res"},{"key":"BFsrep43470_CR9","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/j.preteyeres.2003.12.001","volume":"23","author":"NN Osborne","year":"2004","unstructured":"Osborne, N. N. et al. Retinal ischemia: mechanisms of damage and potential therapeutic strategies. Prog Retin Eye Res 23, 91\u2013147 (2004).","journal-title":"Prog Retin Eye Res"},{"key":"BFsrep43470_CR10","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1007\/s00441-011-1302-7","volume":"349","author":"D Fischer","year":"2012","unstructured":"Fischer, D. Stimulating axonal regeneration of mature retinal ganglion cells and overcoming inhibitory signaling. Cell Tissue Res 349, 79\u201385 (2012).","journal-title":"Cell Tissue Res"},{"key":"BFsrep43470_CR11","doi-asserted-by":"publisher","first-page":"688","DOI":"10.1016\/j.preteyeres.2012.06.005","volume":"31","author":"D Fischer","year":"2012","unstructured":"Fischer, D. & Leibinger, M. Promoting optic nerve regeneration. Prog Retin Eye Res 31, 688\u2013701 (2012).","journal-title":"Prog Retin Eye Res"},{"key":"BFsrep43470_CR12","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1007\/s00441-012-1335-6","volume":"349","author":"CW Schmeer","year":"2012","unstructured":"Schmeer, C. W., Wohl, S. G. & Isenmann, S. Cell-replacement therapy and neural repair in the retina. Cell Tissue Res 349, 363\u2013374 (2012).","journal-title":"Cell Tissue Res"},{"key":"BFsrep43470_CR13","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/j.exer.2014.07.001","volume":"133","author":"J Reinhard","year":"2015","unstructured":"Reinhard, J., Joachim, S. C. & Faissner, A. Extracellular matrix remodeling during retinal development. Exp Eye Res 133, 132\u2013140 (2015).","journal-title":"Exp Eye Res"},{"key":"BFsrep43470_CR14","doi-asserted-by":"publisher","first-page":"832","DOI":"10.1111\/j.1471-4159.1990.tb04567.x","volume":"55","author":"M Schulz","year":"1990","unstructured":"Schulz, M., Raju, T., Ralston, G. & Bennett, M. R. A retinal ganglion cell neurotrophic factor purified from the superior colliculus. J Neurochem 55, 832\u2013841 (1990).","journal-title":"J Neurochem"},{"key":"BFsrep43470_CR15","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1016\/0165-3806(94)00155-S","volume":"84","author":"KR Huxlin","year":"1995","unstructured":"Huxlin, K. R., Carr, R., Schulz, M., Sefton, A. J. & Bennett, M. R. Trophic effect of collicular proteoglycan on neonatal rat retinal ganglion cells in situ . Brain Res Dev Brain Res 84, 77\u201388 (1995).","journal-title":"Brain Res Dev Brain Res"},{"key":"BFsrep43470_CR16","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/0006-8993(95)00695-M","volume":"697","author":"KA Nichol","year":"1995","unstructured":"Nichol, K. A., Schulz, M. W. & Bennett, M. R. Nitric oxide-mediated death of cultured neonatal retinal ganglion cells: neuroprotective properties of glutamate and chondroitin sulfate proteoglycan. Brain Res 697, 1\u201316 (1995).","journal-title":"Brain Res"},{"key":"BFsrep43470_CR17","first-page":"2748","volume":"41","author":"M Inatani","year":"2000","unstructured":"Inatani, M. et al. Upregulated expression of neurocan, a nervous tissue specific proteoglycan, in transient retinal ischemia. Invest Ophthalmol Vis Sci 41, 2748\u20132754 (2000).","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"BFsrep43470_CR18","first-page":"1783","volume":"40","author":"M Inatani","year":"1999","unstructured":"Inatani, M. et al. Expression of proteoglycan decorin in neural retina. Invest Ophthalmol Vis Sci 40, 1783\u20131791 (1999).","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"BFsrep43470_CR19","doi-asserted-by":"publisher","first-page":"2777","DOI":"10.1167\/iovs.13-13372","volume":"55","author":"H Schmid","year":"2014","unstructured":"Schmid, H., Renner, M., Dick, H. B. & Joachim, S. C. Loss of inner retinal neurons after retinal ischemia in rats. Invest Ophthalmol Vis Sci 55, 2777\u20132787 (2014).","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"BFsrep43470_CR20","doi-asserted-by":"publisher","first-page":"783","DOI":"10.1083\/jcb.126.3.783","volume":"126","author":"A Faissner","year":"1994","unstructured":"Faissner, A. et al. Isolation of a neural chondroitin sulfate proteoglycan with neurite outgrowth promoting properties. J Cell Biol 126, 783\u2013799 (1994).","journal-title":"J Cell Biol"},{"key":"BFsrep43470_CR21","doi-asserted-by":"publisher","first-page":"28444","DOI":"10.1074\/jbc.273.43.28444","volume":"273","author":"AM Clement","year":"1998","unstructured":"Clement, A. M. et al. The DSD-1 carbohydrate epitope depends on sulfation, correlates with chondroitin sulfate D motifs, and is sufficient to promote neurite outgrowth. J Biol Chem 273, 28444\u201328453 (1998).","journal-title":"J Biol Chem"},{"key":"BFsrep43470_CR22","doi-asserted-by":"publisher","first-page":"1330","DOI":"10.1002\/glia.22839","volume":"63","author":"A Faissner","year":"2015","unstructured":"Faissner, A. & Reinhard, J. The extracellular matrix compartment of neural stem and glial progenitor cells. Glia 63, 1330\u20131349 (2015).","journal-title":"Glia"},{"key":"BFsrep43470_CR23","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1002\/cne.21479","volume":"504","author":"A Klausmeyer","year":"2007","unstructured":"Klausmeyer, A., Garwood, J. & Faissner, A. Differential expression of phosphacan\/RPTPbeta isoforms in the developing mouse visual system. J Comp Neurol 504, 659\u2013679 (2007).","journal-title":"J Comp Neurol"},{"key":"BFsrep43470_CR24","first-page":"500","volume":"28","author":"SJ Sramek","year":"1987","unstructured":"Sramek, S. J., Wallow, I. H., Bindley, C. & Sterken, G. Fibronectin distribution in the rat eye. An immunohistochemical study. Invest Ophthalmol Vis Sci 28, 500\u2013505 (1987).","journal-title":"An immunohistochemical study. Invest Ophthalmol Vis Sci"},{"key":"BFsrep43470_CR25","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1016\/0014-4835(88)90014-0","volume":"47","author":"E Essner","year":"1988","unstructured":"Essner, E. & Lin, W. L. Immunocytochemical localization of laminin, type IV collagen and fibronectin in rat retinal vessels. Exp Eye Res 47, 317\u2013327 (1988).","journal-title":"Exp Eye Res"},{"key":"BFsrep43470_CR26","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.exer.2014.10.021","volume":"133","author":"PN Bishop","year":"2015","unstructured":"Bishop, P. N. The role of extracellular matrix in retinal vascular development and preretinal neovascularization. Exp Eye Res 133, 30\u201336 (2015).","journal-title":"Exp Eye Res"},{"key":"BFsrep43470_CR27","first-page":"194","volume":"27","author":"JA Jerdan","year":"1986","unstructured":"Jerdan, J. A. & Glaser, B. M. Retinal microvessel extracellular matrix: an immunofluorescent study. Invest Ophthalmol Vis Sci 27, 194\u2013203 (1986).","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"BFsrep43470_CR28","first-page":"258","volume":"37","author":"S Roy","year":"1996","unstructured":"Roy, S., Cagliero, E. & Lorenzi, M. Fibronectin overexpression in retinal microvessels of patients with diabetes. Invest Ophthalmol Vis Sci 37, 258\u2013266 (1996).","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"BFsrep43470_CR29","doi-asserted-by":"publisher","first-page":"3073","DOI":"10.1182\/blood.V90.8.3073","volume":"90","author":"EL George","year":"1997","unstructured":"George, E. L., Baldwin, H. S. & Hynes, R. O. Fibronectins are essential for heart and blood vessel morphogenesis but are dispensable for initial specification of precursor cells. Blood 90, 3073\u20133081 (1997).","journal-title":"Blood"},{"key":"BFsrep43470_CR30","doi-asserted-by":"publisher","first-page":"4451","DOI":"10.1242\/dev.071381","volume":"138","author":"D Stenzel","year":"2011","unstructured":"Stenzel, D. et al. Integrin-dependent and -independent functions of astrocytic fibronectin in retinal angiogenesis. Development 138, 4451\u20134463 (2011).","journal-title":"Development"},{"key":"BFsrep43470_CR31","doi-asserted-by":"publisher","first-page":"568","DOI":"10.1167\/iovs.04-0649","volume":"46","author":"R Fuchshofer","year":"2005","unstructured":"Fuchshofer, R., Birke, M., Welge-Lussen, U., Kook, D. & Lutjen-Drecoll, E. Transforming growth factor-beta 2 modulated extracellular matrix component expression in cultured human optic nerve head astrocytes. Invest Ophthalmol Vis Sci 46, 568\u2013578 (2005).","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"BFsrep43470_CR32","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.matbio.2011.12.002","volume":"31","author":"YN Li","year":"2012","unstructured":"Li, Y. N. et al. The gamma3 chain of laminin is widely but differentially expressed in murine basement membranes: expression and functional studies. Matrix Biol 31, 120\u2013134 (2012).","journal-title":"Matrix Biol"},{"key":"BFsrep43470_CR33","doi-asserted-by":"publisher","first-page":"47461","DOI":"10.1074\/jbc.M204824200","volume":"277","author":"SK Chintala","year":"2002","unstructured":"Chintala, S. K., Zhang, X., Austin, J. S. & Fini, M. E. Deficiency in matrix metalloproteinase gelatinase B (MMP-9) protects against retinal ganglion cell death after optic nerve ligation. J Biol Chem 277, 47461\u201347468 (2002).","journal-title":"J Biol Chem"},{"key":"BFsrep43470_CR34","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1167\/iovs.04-0832","volume":"46","author":"L Guo","year":"2005","unstructured":"Guo, L. et al. Retinal ganglion cell apoptosis in glaucoma is related to intraocular pressure and IOP-induced effects on extracellular matrix. Invest Ophthalmol Vis Sci 46, 175\u2013182 (2005).","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"BFsrep43470_CR35","doi-asserted-by":"publisher","first-page":"e0125250","DOI":"10.1371\/journal.pone.0125250","volume":"10","author":"E Vecino","year":"2015","unstructured":"Vecino, E., Heller, J. P., Veiga-Crespo, P., Martin, K. R. & Fawcett, J. W. Influence of extracellular matrix components on the expression of integrins and regeneration of adult retinal ganglion cells. PLoS One 10, e0125250 (2015).","journal-title":"PLoS One"},{"key":"BFsrep43470_CR36","doi-asserted-by":"publisher","first-page":"e48332","DOI":"10.1371\/journal.pone.0048332","volume":"7","author":"AR Santos","year":"2012","unstructured":"Santos, A. R. et al. beta1 integrin-focal adhesion kinase (FAK) signaling modulates retinal ganglion cell (RGC) survival. PLoS One 7, e48332 (2012).","journal-title":"PLoS One"},{"key":"BFsrep43470_CR37","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1016\/S0002-9440(10)64374-X","volume":"160","author":"D Gebarowska","year":"2002","unstructured":"Gebarowska, D. et al. Synthetic peptides interacting with the 67-kd laminin receptor can reduce retinal ischemia and inhibit hypoxia-induced retinal neovascularization. Am J Pathol 160, 307\u2013313 (2002).","journal-title":"Am J Pathol"},{"key":"BFsrep43470_CR38","doi-asserted-by":"publisher","first-page":"911","DOI":"10.3109\/02713689508995131","volume":"14","author":"L D\u2019Alessandri","year":"1995","unstructured":"D\u2019Alessandri, L., Ranscht, B., Winterhalter, K. H. & Vaughan, L. Contactin\/F11 and tenascin-C co-expression in the chick retina correlates with formation of the synaptic plexiform layers. Curr Eye Res 14, 911\u2013926 (1995).","journal-title":"Curr Eye Res"},{"key":"BFsrep43470_CR39","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/B978-0-444-63486-3.00002-5","volume":"214","author":"A Heikkinen","year":"2014","unstructured":"Heikkinen, A., Pihlajaniemi, T., Faissner, A. & Yuzaki, M. Neural ECM and synaptogenesis. Prog Brain Res 214, 29\u201351 (2014).","journal-title":"Prog Brain Res"},{"key":"BFsrep43470_CR40","doi-asserted-by":"publisher","first-page":"5214961","DOI":"10.1155\/2016\/5214961","volume":"2016","author":"E Dzyubenko","year":"2016","unstructured":"Dzyubenko, E., Gottschling, C. & Faissner, A. Neuron-Glia Interactions in Neural Plasticity: Contributions of Neural Extracellular Matrix and Perineuronal Nets. Neural Plast 2016, 5214961 (2016).","journal-title":"Neural Plast"},{"key":"BFsrep43470_CR41","doi-asserted-by":"publisher","first-page":"858","DOI":"10.1016\/j.exer.2007.01.006","volume":"84","author":"F Dijk","year":"2007","unstructured":"Dijk, F., Bergen, A. A. & Kamphuis, W. GAP-43 expression is upregulated in retinal ganglion cells after ischemia\/reperfusion-induced damage. Exp Eye Res 84, 858\u2013867 (2007).","journal-title":"Exp Eye Res"},{"key":"BFsrep43470_CR42","doi-asserted-by":"publisher","first-page":"2125","DOI":"10.1002\/hep.24639","volume":"54","author":"N Kuriyama","year":"2011","unstructured":"Kuriyama, N., Duarte, S., Hamada, T., Busuttil, R. W. & Coito, A. J. Tenascin-C: a novel mediator of hepatic ischemia and reperfusion injury. Hepatology 54, 2125\u20132136 (2011).","journal-title":"Hepatology"},{"key":"BFsrep43470_CR43","doi-asserted-by":"publisher","first-page":"1116","DOI":"10.2967\/jnumed.109.071340","volume":"51","author":"J Taki","year":"2010","unstructured":"Taki, J. et al. Dynamic expression of tenascin-C after myocardial ischemia and reperfusion: assessment by 125I-anti-tenascin-C antibody imaging. J Nucl Med 51, 1116\u20131122 (2010).","journal-title":"J Nucl Med"},{"key":"BFsrep43470_CR44","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1186\/s13550-015-0100-8","volume":"5","author":"J Taki","year":"2015","unstructured":"Taki, J. et al. Effect of postconditioning on dynamic expression of tenascin-C and left ventricular remodeling after myocardial ischemia and reperfusion. EJNMMI Res 5, 21 (2015).","journal-title":"EJNMMI Res"},{"key":"BFsrep43470_CR45","doi-asserted-by":"publisher","first-page":"786","DOI":"10.1097\/01.WCB.0000062340.80057.06","volume":"23","author":"A Lu","year":"2003","unstructured":"Lu, A. et al. Genomics of the periinfarction cortex after focal cerebral ischemia. J Cereb Blood Flow Metab 23, 786\u2013810 (2003).","journal-title":"J Cereb Blood Flow Metab"},{"key":"BFsrep43470_CR46","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/978-3-319-04981-6_20","volume":"120","author":"H Suzuki","year":"2015","unstructured":"Suzuki, H. et al. Tenascin-C is a possible mediator between initial brain injury and vasospasm-related and -unrelated delayed cerebral ischemia after aneurysmal subarachnoid hemorrhage. Acta Neurochir Suppl 120, 117\u2013121 (2015).","journal-title":"Acta Neurochir Suppl"},{"key":"BFsrep43470_CR47","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1002\/(SICI)1098-1136(199903)26:1<36::AID-GLIA4>3.0.CO;2-C","volume":"26","author":"S Hirsch","year":"1999","unstructured":"Hirsch, S. & Bahr, M. Immunocytochemical characterization of reactive optic nerve astrocytes and meningeal cells. Glia 26, 36\u201346 (1999).","journal-title":"Glia"},{"key":"BFsrep43470_CR48","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1007\/s00441-011-1313-4","volume":"349","author":"L Roll","year":"2012","unstructured":"Roll, L., Mittmann, T., Eysel, U. T. & Faissner, A. The laser lesion of the mouse visual cortex as a model to study neural extracellular matrix remodeling during degeneration, regeneration and plasticity of the CNS. Cell Tissue Res 349, 133\u2013145 (2012).","journal-title":"Cell Tissue Res"},{"key":"BFsrep43470_CR49","doi-asserted-by":"publisher","first-page":"219","DOI":"10.3389\/fncel.2014.00219","volume":"8","author":"L Roll","year":"2014","unstructured":"Roll, L. & Faissner, A. Influence of the extracellular matrix on endogenous and transplanted stem cells after brain damage. Front Cell Neurosci 8, 219 (2014).","journal-title":"Front Cell Neurosci"},{"key":"BFsrep43470_CR50","doi-asserted-by":"publisher","first-page":"3161","DOI":"10.1167\/iovs.06-1282","volume":"48","author":"EC Johnson","year":"2007","unstructured":"Johnson, E. C., Jia, L., Cepurna, W. O., Doser, T. A. & Morrison, J. C. Global changes in optic nerve head gene expression after exposure to elevated intraocular pressure in a rat glaucoma model. Invest Ophthalmol Vis Sci 48, 3161\u20133177 (2007).","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"BFsrep43470_CR51","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1006\/exer.1998.0577","volume":"68","author":"JD Pena","year":"1999","unstructured":"Pena, J. D., Varela, H. J., Ricard, C. S. & Hernandez, M. R. Enhanced tenascin expression associated with reactive astrocytes in human optic nerve heads with primary open angle glaucoma. Exp Eye Res 68, 29\u201340 (1999).","journal-title":"Exp Eye Res"},{"key":"BFsrep43470_CR52","doi-asserted-by":"crossref","unstructured":"Reinehr, S. et al. Early remodelling of the extracellular matrix proteins tenascin-C and phosphacan in retina and optic nerve of an experimental autoimmune glaucoma model. J Cell Mol Med(2016).","DOI":"10.1111\/jcmm.12909"},{"key":"BFsrep43470_CR53","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1016\/S0301-0082(99)00061-1","volume":"61","author":"P Pesheva","year":"2000","unstructured":"Pesheva, P. & Probstmeier, R. The yin and yang of tenascin-R in CNS development and pathology. Prog Neurobiol 61, 465\u2013493 (2000).","journal-title":"Prog Neurobiol"},{"key":"BFsrep43470_CR54","doi-asserted-by":"publisher","first-page":"26641","DOI":"10.1074\/jbc.R500005200","volume":"280","author":"HC Hsia","year":"2005","unstructured":"Hsia, H. C. & Schwarzbauer, J. E. Meet the tenascins: multifunctional and mysterious. J Biol Chem 280, 26641\u201326644 (2005).","journal-title":"J Biol Chem"},{"key":"BFsrep43470_CR55","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1002\/glia.440090108","volume":"9","author":"U Bartsch","year":"1993","unstructured":"Bartsch, U., Pesheva, P., Raff, M. & Schachner, M. Expression of janusin (J1-160\/180) in the retina and optic nerve of the developing and adult mouse. Glia 9, 57\u201369 (1993).","journal-title":"Glia"},{"key":"BFsrep43470_CR56","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1111\/j.1460-9568.1993.tb00497.x","volume":"5","author":"ES Wintergerst","year":"1993","unstructured":"Wintergerst, E. S., Fuss, B. & Bartsch, U. Localization of janusin mRNA in the central nervous system of the developing and adult mouse. Eur J Neurosci 5, 299\u2013310 (1993).","journal-title":"Eur J Neurosci"},{"key":"BFsrep43470_CR57","doi-asserted-by":"publisher","first-page":"330","DOI":"10.1002\/(SICI)1098-1136(20000215)29:4<330::AID-GLIA4>3.0.CO;2-L","volume":"29","author":"T Becker","year":"2000","unstructured":"Becker, T. et al. Tenascin-R inhibits regrowth of optic fibers in vitro and persists in the optic nerve of mice after injury. Glia 29, 330\u2013346 (2000).","journal-title":"Glia"},{"key":"BFsrep43470_CR58","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1523\/JNEUROSCI.19-02-00813.1999","volume":"19","author":"CG Becker","year":"1999","unstructured":"Becker, C. G., Becker, T., Meyer, R. L. & Schachner, M. Tenascin-R inhibits the growth of optic fibers in vitro but is rapidly eliminated during nerve regeneration in the salamander Pleurodeles waltl. J Neurosci 19, 813\u2013827 (1999).","journal-title":"J Neurosci"},{"key":"BFsrep43470_CR59","doi-asserted-by":"publisher","first-page":"842","DOI":"10.1523\/JNEUROSCI.22-03-00842.2002","volume":"22","author":"CG Becker","year":"2002","unstructured":"Becker, C. G. & Becker, T. Repellent guidance of regenerating optic axons by chondroitin sulfate glycosaminoglycans in zebrafish. J Neurosci 22, 842\u2013853 (2002).","journal-title":"J Neurosci"},{"key":"BFsrep43470_CR60","doi-asserted-by":"publisher","first-page":"899","DOI":"10.1002\/dneu.20624","volume":"68","author":"DM Lang","year":"2008","unstructured":"Lang, D. M. et al. Tenascin-R and axon growth-promoting molecules are up-regulated in the regenerating visual pathway of the lizard (Gallotia galloti). Dev Neurobiol 68, 899\u2013916 (2008).","journal-title":"Dev Neurobiol"},{"key":"BFsrep43470_CR61","doi-asserted-by":"publisher","first-page":"7337","DOI":"10.1074\/jbc.M007234200","volume":"276","author":"S Schumacher","year":"2001","unstructured":"Schumacher, S. et al. CALEB binds via its acidic stretch to the fibrinogen-like domain of tenascin-C or tenascin-R and its expression is dynamically regulated after optic nerve lesion. J Biol Chem 276, 7337\u20137345 (2001).","journal-title":"J Biol Chem"},{"key":"BFsrep43470_CR62","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1016\/S1350-9462(02)00009-5","volume":"21","author":"M Inatani","year":"2002","unstructured":"Inatani, M. & Tanihara, H. Proteoglycans in retina. Prog Retin Eye Res 21, 429\u2013447 (2002).","journal-title":"Prog Retin Eye Res"},{"key":"BFsrep43470_CR63","first-page":"658","volume":"21","author":"MM LaVail","year":"1981","unstructured":"LaVail, M. M., Pinto, L. H. & Yasumura, D. The interphotoreceptor matrix in rats with inherited retinal dystrophy. Invest Ophthalmol Vis Sci 21, 658\u2013668 (1981).","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"BFsrep43470_CR64","doi-asserted-by":"publisher","first-page":"981","DOI":"10.3109\/02713688608995180","volume":"5","author":"K Porrello","year":"1986","unstructured":"Porrello, K. & LaVail, M. M. Immunocytochemical localization of chondroitin sulfates in the interphotoreceptor matrix of the normal and dystrophic rat retina. Curr Eye Res 5, 981\u2013993 (1986).","journal-title":"Curr Eye Res"},{"key":"BFsrep43470_CR65","doi-asserted-by":"publisher","first-page":"169","DOI":"10.3109\/02713688509000846","volume":"4","author":"AT Hewitt","year":"1985","unstructured":"Hewitt, A. T. & Newsome, D. A. Altered synthesis of Bruch\u2019s membrane proteoglycans associated with dominant retinitis pigmentosa. Curr Eye Res 4, 169\u2013174 (1985).","journal-title":"Curr Eye Res"},{"key":"BFsrep43470_CR66","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/0042-6989(94)E0049-Q","volume":"35","author":"J Wallman","year":"1995","unstructured":"Wallman, J. et al. Moving the retina: choroidal modulation of refractive state. Vision Res 35, 37\u201350 (1995).","journal-title":"Vision Res"},{"key":"BFsrep43470_CR67","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1006\/exnr.2000.7573","volume":"167","author":"I Selles-Navarro","year":"2001","unstructured":"Selles-Navarro, I., Ellezam, B., Fajardo, R., Latour, M. & McKerracher, L. Retinal ganglion cell and nonneuronal cell responses to a microcrush lesion of adult rat optic nerve. Exp Neurol 167, 282\u2013289 (2001).","journal-title":"Exp Neurol"},{"key":"BFsrep43470_CR68","doi-asserted-by":"publisher","first-page":"488","DOI":"10.1016\/j.expneurol.2012.01.023","volume":"234","author":"LF Chen","year":"2012","unstructured":"Chen, L. F., FitzGibbon, T., He, J. R. & Yin, Z. Q. Localization and developmental expression patterns of CSPG-cs56 (aggrecan) in normal and dystrophic retinas in two rat strains. Exp Neurol 234, 488\u2013498 (2012).","journal-title":"Exp Neurol"},{"key":"BFsrep43470_CR69","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/j.brainres.2005.07.036","volume":"1056","author":"S Nagel","year":"2005","unstructured":"Nagel, S. et al. Focal cerebral ischemia induces changes in both MMP-13 and aggrecan around individual neurons. Brain Res 1056, 43\u201350 (2005).","journal-title":"Brain Res"},{"key":"BFsrep43470_CR70","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1016\/j.expneurol.2013.06.011","volume":"248","author":"T Kuboyama","year":"2013","unstructured":"Kuboyama, T. et al. Paxillin phosphorylation counteracts proteoglycan-mediated inhibition of axon regeneration. Exp Neurol 248, 157\u2013169 (2013).","journal-title":"Exp Neurol"},{"key":"BFsrep43470_CR71","doi-asserted-by":"publisher","first-page":"1266","DOI":"10.1111\/j.1471-4159.2009.05873.x","volume":"108","author":"Y Bekku","year":"2009","unstructured":"Bekku, Y., Rauch, U., Ninomiya, Y. & Oohashi, T. Brevican distinctively assembles extracellular components at the large diameter nodes of Ranvier in the CNS. J Neurochem 108, 1266\u20131276 (2009).","journal-title":"J Neurochem"},{"key":"BFsrep43470_CR72","doi-asserted-by":"publisher","first-page":"3113","DOI":"10.1523\/JNEUROSCI.5598-09.2010","volume":"30","author":"Y Bekku","year":"2010","unstructured":"Bekku, Y. et al. Bral1: its role in diffusion barrier formation and conduction velocity in the CNS. J Neurosci 30, 3113\u20133123 (2010).","journal-title":"J Neurosci"},{"key":"BFsrep43470_CR73","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/j.expneurol.2004.11.021","volume":"193","author":"J Aya-ay","year":"2005","unstructured":"Aya-ay, J. et al. The effect of hypoxic-ischemic brain injury in perinatal rats on the abundance and proteolysis of brevican and NG2. Exp Neurol 193, 149\u2013162 (2005).","journal-title":"Exp Neurol"},{"key":"BFsrep43470_CR74","doi-asserted-by":"publisher","first-page":"1106","DOI":"10.1002\/jnr.21553","volume":"86","author":"CC Leonardo","year":"2008","unstructured":"Leonardo, C. C., Eakin, A. K., Ajmo, J. M. & Gottschall, P. E. Versican and brevican are expressed with distinct pathology in neonatal hypoxic-ischemic injury. J Neurosci Res 86, 1106\u20131114 (2008).","journal-title":"J Neurosci Res"},{"key":"BFsrep43470_CR75","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1007\/s00401-011-0914-z","volume":"123","author":"R Reitmeir","year":"2012","unstructured":"Reitmeir, R. et al. Vascular endothelial growth factor induces contralesional corticobulbar plasticity and functional neurological recovery in the ischemic brain. Acta Neuropathol 123, 273\u2013284 (2012).","journal-title":"Acta Neuropathol"},{"key":"BFsrep43470_CR76","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/j.expneurol.2008.11.026","volume":"216","author":"M Fitzgerald","year":"2009","unstructured":"Fitzgerald, M. et al. Secondary degeneration of the optic nerve following partial transection: the benefits of lomerizine. Exp Neurol 216, 219\u2013230 (2009).","journal-title":"Exp Neurol"},{"key":"BFsrep43470_CR77","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.brainres.2011.06.005","volume":"1403","author":"KJ Cho","year":"2011","unstructured":"Cho, K. J., Kim, J. H., Park, H. Y. & Park, C. K. Glial cell response and iNOS expression in the optic nerve head and retina of the rat following acute high IOP ischemia-reperfusion. Brain Res 1403, 67\u201377 (2011).","journal-title":"Brain Res"},{"key":"BFsrep43470_CR78","doi-asserted-by":"crossref","first-page":"14349","DOI":"10.1016\/S0021-9258(17)36625-5","volume":"269","author":"G Barnea","year":"1994","unstructured":"Barnea, G. et al. Receptor tyrosine phosphatase beta is expressed in the form of proteoglycan and binds to the extracellular matrix protein tenascin. J Biol Chem 269, 14349\u201314352 (1994).","journal-title":"J Biol Chem"},{"key":"BFsrep43470_CR79","doi-asserted-by":"crossref","first-page":"12142","DOI":"10.1016\/S0021-9258(17)32692-3","volume":"269","author":"M Grumet","year":"1994","unstructured":"Grumet, M. et al. Interactions with tenascin and differential effects on cell adhesion of neurocan and phosphacan, two major chondroitin sulfate proteoglycans of nervous tissue. J Biol Chem 269, 12142\u201312146 (1994).","journal-title":"J Biol Chem"},{"key":"BFsrep43470_CR80","doi-asserted-by":"publisher","first-page":"3468","DOI":"10.1167\/iovs.10-5763","volume":"52","author":"SC Joachim","year":"2011","unstructured":"Joachim, S. C. et al. Upregulation of antibody response to heat shock proteins and tissue antigens in an ocular ischemia model. Invest Ophthalmol Vis Sci 52, 3468\u20133474 (2011).","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"BFsrep43470_CR81","doi-asserted-by":"publisher","first-page":"3626","DOI":"10.1167\/iovs.15-18999R2","volume":"57","author":"C Casola","year":"2016","unstructured":"Casola, C. et al. Specific Inner Retinal Layer Cell Damage in an Autoimmune Glaucoma Model Is Induced by GDNF With or Without HSP27. Invest Ophthalmol Vis Sci 57, 3626\u20133639 (2016).","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"BFsrep43470_CR82","doi-asserted-by":"publisher","first-page":"1790","DOI":"10.1002\/glia.20891","volume":"57","author":"T Czopka","year":"2009","unstructured":"Czopka, T., Von Holst, A., Schmidt, G., Ffrench-Constant, C. & Faissner, A. Tenascin C and tenascin R similarly prevent the formation of myelin membranes in a RhoA-dependent manner, but antagonistically regulate the expression of myelin basic protein via a separate pathway. Glia 57, 1790\u20131801 (2009).","journal-title":"Glia"},{"key":"BFsrep43470_CR83","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1016\/j.neuroscience.2016.01.039","volume":"319","author":"M Kwiatkowska","year":"2016","unstructured":"Kwiatkowska, M. et al. The expression pattern and inhibitory influence of Tenascin-C on the growth of spiral ganglion neurons suggest a regulatory role as boundary formation molecule in the postnatal mouse inner ear. Neuroscience 319, 46\u201358 (2016).","journal-title":"Neuroscience"},{"key":"BFsrep43470_CR84","doi-asserted-by":"publisher","first-page":"627","DOI":"10.1016\/0896-6273(90)90217-4","volume":"5","author":"A Faissner","year":"1990","unstructured":"Faissner, A. & Kruse, J. J1\/tenascin is a repulsive substrate for central nervous system neurons. Neuron 5, 627\u2013637 (1990).","journal-title":"Neuron"},{"key":"BFsrep43470_CR85","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1242\/dev.113.1.151","volume":"113","author":"FG Rathjen","year":"1991","unstructured":"Rathjen, F. G., Wolff, J. M. & Chiquet-Ehrismann, R. Restrictin: a chick neural extracellular matrix protein involved in cell attachment co-purifies with the cell recognition molecule F11. Development 113, 151\u2013164 (1991).","journal-title":"Development"},{"key":"BFsrep43470_CR86","doi-asserted-by":"publisher","first-page":"27206","DOI":"10.1074\/jbc.270.45.27206","volume":"270","author":"CI Seidenbecher","year":"1995","unstructured":"Seidenbecher, C. I. et al. Brevican, a chondroitin sulfate proteoglycan of rat brain, occurs as secreted and cell surface glycosylphosphatidylinositol-anchored isoforms. J Biol Chem 270, 27206\u201327212 (1995).","journal-title":"J Biol Chem"}],"container-title":["Scientific Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/srep43470.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep43470","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/srep43470.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T13:41:43Z","timestamp":1692711703000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/srep43470"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,6]]},"references-count":86,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,4,24]]}},"alternative-id":["BFsrep43470"],"URL":"https:\/\/doi.org\/10.1038\/srep43470","relation":{},"ISSN":["2045-2322"],"issn-type":[{"value":"2045-2322","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,6]]},"assertion":[{"value":"24 August 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"25 January 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 March 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"43470"}}