{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T22:35:23Z","timestamp":1789511723560,"version":"build-2803163510"},"reference-count":44,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,3,3]],"date-time":"2017-03-03T00:00:00Z","timestamp":1488499200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,3,3]],"date-time":"2017-03-03T00:00:00Z","timestamp":1488499200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci Rep"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Osteoarthritis (OA) is a common joint disorder with varying degrees of inflammation and sustained oxidative stress. The root extract of <jats:italic>Scutellaria baicalensis<\/jats:italic> (SBE) has been used for the treatment of inflammatory and other diseases. Here, we performed activity-guided HPLC-fractionation of SBE, identified the active ingredient(s) and investigated its chondroprotective potential. We found that the Wogonin containing fraction-4 (F4) was the most potent fraction based on its ability to inhibit ROS production and the suppression of catabolic markers including IL-6, COX-2, iNOS, MMP-3, MMP-9, MMP-13 and ADAMTS-4 in IL-1\u03b2-treated OA chondrocytes. OA chondrocytes treated with F4 in the presence of IL-1\u03b2 showed significantly enhanced expression of anabolic genes ACAN and COL2A1. In an <jats:italic>in vitro<\/jats:italic> model of cartilage degradation treatment with F4 inhibited s-GAG release from IL-1\u03b2-treated human cartilage explants. The inhibitory effect of F4 was not mediated through the inhibition of MAPKs and NF-\u03baB activation but was mediated through the suppression of c-Fos\/AP-1 activity at transcriptional and post transcriptional levels in OA chondrocytes. Purified Wogonin mimicked the effects of F4 in IL-1\u03b2-stimulated OA chondrocytes. Our data demonstrates that a Wogonin-rich fraction of SBE exert chondroprotective effects through the suppression of c-Fos\/AP-1 expression and activity in OA chondrocytes under pathological conditions.<\/jats:p>","DOI":"10.1038\/srep43789","type":"journal-article","created":{"date-parts":[[2017,3,3]],"date-time":"2017-03-03T12:20:51Z","timestamp":1488543651000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["A wogonin-rich-fraction of Scutellaria baicalensis root extract exerts chondroprotective effects by suppressing IL-1\u03b2-induced activation of AP-1 in human OA chondrocytes"],"prefix":"10.1038","volume":"7","author":[{"given":"Nazir M.","family":"Khan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Haseeb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Y.","family":"Ansari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tariq M.","family":"Haqqi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,3,3]]},"reference":[{"key":"BFsrep43789_CR1","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1016\/S1297-319X(00)00216-5","volume":"67","author":"WB Van den Berg","year":"2000","unstructured":"Van den Berg, W. 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