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The muscle specific caveolin3 (Cav-3) and the caveolae have been found to be critical for cardioprotection. This study aimed to evaluate our hypothesis that caveolae and Cav-3 are essential for AS-1-induced cardioprotection against myocardial I\/R injury. To address these issues, we analyzed the involvement of Cav-3 in AS-1 mediated cardioprotection both <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>. We demonstrate that AS-1 administration significantly decreased infarct size, improved cardiac function after myocardial I\/R and modulated membrane caveolae and Cav-3 expression in the myocardium. For <jats:italic>in vitro<\/jats:italic> studies, AS-1 treatment prevented Cav-3 re-distribution induced by H\/R injury. In contrast, disruption of caveolae by MCD treatment or Cav-3 knockdown abolished the protection against H\/R-induced myocytes injury by AS-1. Our findings reveal that AS-1 attenuates myocardial I\/R injury through caveolae and Cav-3 dependent mechanism.<\/jats:p>","DOI":"10.1038\/srep44638","type":"journal-article","created":{"date-parts":[[2017,3,14]],"date-time":"2017-03-14T15:42:48Z","timestamp":1489506168000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["TIR\/BB-loop mimetic AS-1 attenuates cardiac ischemia\/reperfusion injury via a caveolae and caveolin-3-dependent mechanism"],"prefix":"10.1038","volume":"7","author":[{"given":"Yuanping","family":"Hu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meiling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoliang","family":"Dai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danyang","family":"Ren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linli","family":"Que","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tuanzhu","family":"Ha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuanfu","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenzheng","family":"Ju","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuehua","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,3,14]]},"reference":[{"key":"BFsrep44638_CR1","doi-asserted-by":"publisher","first-page":"894","DOI":"10.1038\/nm0897-894","volume":"3","author":"R Morishita","year":"1997","unstructured":"R. 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