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Their binding partner laminin 332 as well as integrin \u03b16\u03b24 are largely recognized to promote invasion and metastasis in various cancers, and collagen XVII is essential for the survival of colon and lung cancer stem cells. We have studied the expression of laminin \u03b32, collagen XVII and integrin \u03b24 in tissue microarray samples of squamous cell carcinoma (SCC) and its precursors, actinic keratosis and Bowen\u2019s disease. The expression of laminin \u03b32 was highest in SCC samples, whereas the expression of collagen XVII and integrin \u03b24 varied greatly in SCC and its precursors. Collagen XVII and integrin \u03b24 were also expressed in SCC cell lines. Virus-mediated RNAi knockdown of collagen XVII and integrin \u03b24 reduced the migration of less aggressive SCC-25 cells in horizontal scratch wound healing assay. Additionally, in a 3D organotypic myoma invasion assay the loss of collagen XVII or integrin \u03b24 suppressed equally the migration and invasion of SCC-25 cells whereas there was no effect on the most aggressive HSC-3 cells. Variable expression patterns and results in migration and invasion assays suggest that collagen XVII and integrin \u03b24 contribute to SCC tumorigenesis.<\/jats:p>","DOI":"10.1038\/srep45057","type":"journal-article","created":{"date-parts":[[2017,3,22]],"date-time":"2017-03-22T12:19:20Z","timestamp":1490185160000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Significant Role of Collagen XVII And Integrin \u03b24 in Migration and Invasion of The Less Aggressive Squamous Cell Carcinoma Cells"],"prefix":"10.1038","volume":"7","author":[{"given":"Jyri M.","family":"Moilanen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefanie","family":"L\u00f6ffek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina","family":"Kokkonen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sirpa","family":"Salo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juha P.","family":"V\u00e4yrynen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiina","family":"Hurskainen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aki","family":"Manninen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pilvi","family":"Riihil\u00e4","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ritva","family":"Heljasvaara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claus-Werner","family":"Franzke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veli-Matti","family":"K\u00e4h\u00e4ri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tuula","family":"Salo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus J.","family":"M\u00e4kinen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaisa","family":"Tasanen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,3,22]]},"reference":[{"key":"BFsrep45057_CR1","doi-asserted-by":"publisher","first-page":"902","DOI":"10.1245\/ASO.2006.07.022","volume":"13","author":"JT Mullen","year":"2006","unstructured":"Mullen, J. 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