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The 33-mer peptide from \u03b12-gliadin has frequently been described as the most important CD-immunogenic sequence within gluten. However, from more than 890 published amino acid sequences of \u03b1-gliadins, only 19 sequences contain the 33-mer. In order to make a precise assessment of the importance of the 33-mer, it is necessary to elucidate which wheat species and cultivars contain the peptide and at which concentrations. This paper presents the development of a stable isotope dilution assay followed by liquid chromatography tandem mass spectrometry to quantitate the 33-mer in flours of 23 hexaploid modern and 15 old common (bread) wheat as well as two spelt cultivars. All flours contained the 33-mer peptide at levels ranging from 91\u2013603\u2009\u03bcg\/g flour. In contrast, the 33-mer was absent (&lt;limit of detection) from tetra- and diploid species (durum wheat, emmer, einkorn), most likely because of the absence of the D-genome, which encodes \u03b12-gliadins. Due to the presence of the 33-mer in all common wheat and spelt flours analysed here, the special focus in the literature on this most immunodominant peptide seems to be justified.<\/jats:p>","DOI":"10.1038\/srep45092","type":"journal-article","created":{"date-parts":[[2017,3,22]],"date-time":"2017-03-22T11:40:17Z","timestamp":1490182817000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":93,"title":["Quantitation of the immunodominant 33-mer peptide from \u03b1-gliadin in wheat flours by liquid chromatography tandem mass spectrometry"],"prefix":"10.1038","volume":"7","author":[{"given":"Kathrin","family":"Schalk","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Lang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herbert","family":"Wieser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Koehler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina Anne","family":"Scherf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,3,22]]},"reference":[{"key":"BFsrep45092_CR1","doi-asserted-by":"publisher","first-page":"587","DOI":"10.3109\/07853890.2010.505931","volume":"42","author":"K Mustalahti","year":"2010","unstructured":"Mustalahti, K. et al. 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