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Persistent infections are seen from the first <jats:italic>P. aeruginosa<\/jats:italic> culture in about 75% of young CF patients, and it is important to discover new ways to detect <jats:italic>P. aeruginosa<\/jats:italic> at an earlier stage. The <jats:italic>P. aeruginosa<\/jats:italic> biomarker hydrogen cyanide (HCN) contains a triple bond, which is utilized in this study because of the resulting characteristic C\u2261N peak at 2135\u2009cm<jats:sup>\u22121<\/jats:sup> in a Raman spectrum. The Raman signal was enhanced by surface-enhanced Raman spectroscopy (SERS) on a Au-coated SERS substrate. After long-term infection, a mutation in the patho-adaptive <jats:italic>lasR<\/jats:italic> gene can alter the expression of HCN, which is why it is sometimes not possible to detect HCN in the breath of chronically infected patients. Four <jats:italic>P. aeruginosa<\/jats:italic> reference strains and 12 clinical <jats:italic>P. aeruginosa<\/jats:italic> strains isolated from CF children were evaluated, and HCN was clearly detected from overnight cultures of all wild type-like isolates and half of the later isolates from the same patients. The clinical impact could be that <jats:italic>P. aeruginosa<\/jats:italic> infections could be detected at an earlier stage, because daily breath sampling with an immediate output could be possible with a point-of-care SERS device.<\/jats:p>","DOI":"10.1038\/srep45264","type":"journal-article","created":{"date-parts":[[2017,3,28]],"date-time":"2017-03-28T12:00:46Z","timestamp":1490702446000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["SERS detection of the biomarker hydrogen cyanide from Pseudomonas aeruginosa cultures isolated from cystic fibrosis patients"],"prefix":"10.1038","volume":"7","author":[{"given":"Rikke Kragh","family":"Lauridsen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea M.","family":"Sommer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helle Krogh","family":"Johansen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomas","family":"Rindzevicius","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00f8ren","family":"Molin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Jelsbak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00f8ren Balling","family":"Engelsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anja","family":"Boisen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,3,28]]},"reference":[{"key":"BFsrep45264_CR1","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1002\/ppul.23295","volume":"50","author":"H Johansen","year":"2015","unstructured":"Johansen, H. et al. 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