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However, little is known about TOR in tetraploid species such as cotton. Here, we show that TORC1-S6K-RPS6, the major signaling components, are conserved and further expanded in cotton genome. Though the cotton seedlings are insensitive to rapamycin, AZD8055, the second-generation inhibitor of TOR, can significantly suppress the growth in cotton. Global transcriptome analysis revealed that genes associated with jasmonic acid (JA) biosynthesis and transduction were significantly altered in AZD8055 treated cotton seedlings, suggesting the potential crosstalk between TOR and JA signaling. Pharmacological and genetic approaches have been employed to get further insights into the molecular mechanism of the crosstalk between TOR and JA. Combination of AZD8055 with methyl jasmonate can synergistically inhibit cotton growth, and additionally JA levels were significantly increased when cotton seedlings were subjected to AZD8055. JA biosynthetic and signaling mutants including <jats:italic>jar1, coi1-2<\/jats:italic> and <jats:italic>myc2-2<\/jats:italic> displayed TOR inhibitor-resistant phenotypes, whereas <jats:italic>COI1<\/jats:italic> overexpression transgenic lines and <jats:italic>jaz10<\/jats:italic> exhibited sensitivity to AZD8055. Consistently, cotton <jats:italic>JAZ<\/jats:italic> can partially rescue TOR-suppressed phenotypes in <jats:italic>Arabidopsis<\/jats:italic>. These evidences revealed that the crosstalk between TOR and JA pathway operates in cotton and <jats:italic>Arabidopsis<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/srep45830","type":"journal-article","created":{"date-parts":[[2017,4,4]],"date-time":"2017-04-04T12:07:51Z","timestamp":1491307671000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["The crosstalk between Target of Rapamycin (TOR) and Jasmonic Acid (JA) signaling existing in Arabidopsis and cotton"],"prefix":"10.1038","volume":"7","author":[{"given":"Yun","family":"Song","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linxuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangjie","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengping","family":"Zhuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaojun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuoren","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raju","family":"Datla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maozhi","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuguang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,4,4]]},"reference":[{"key":"BFsrep45830_CR1","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1146\/annurev-arplant-043014-114648","volume":"67","author":"T Dobrenel","year":"2016","unstructured":"Dobrenel, T. et al. 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