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Biofilm-embedded bacteria are generally inaccessible for antimicrobials, therefore the disruption of biofilm matrix is the potent approach to eradicate microbial biofilms. We demonstrate here the destruction of <jats:italic>Staphylococcus aureus<\/jats:italic> and <jats:italic>Staphylococcus epidermidis<\/jats:italic> biofilms with Ficin, a nonspecific plant protease. The biofilm thickness decreased two-fold after 24\u2009hours treatment with Ficin at 10\u2009\u03bcg\/ml and six-fold at 1000\u2009\u03bcg\/ml concentration. We confirmed the successful destruction of biofilm structures and the significant decrease of non-specific bacterial adhesion to the surfaces after Ficin treatment using confocal laser scanning and atomic force microscopy. Importantly, Ficin treatment enhanced the effects of antibiotics on biofilms-embedded cells via disruption of biofilm matrices. Pre-treatment with Ficin (1000\u2009\u03bcg\/ml) considerably reduced the concentrations of ciprofloxacin and bezalkonium chloride required to suppress the viable Staphylococci by 3 orders of magnitude. We also demonstrated that Ficin is not cytotoxic towards human breast adenocarcinoma cells (MCF7) and dog adipose derived stem cells. Overall, Ficin is a potent tool for staphylococcal biofilm treatment and fabrication of novel antimicrobial therapeutics for medical and veterinary applications.<\/jats:p>","DOI":"10.1038\/srep46068","type":"journal-article","created":{"date-parts":[[2017,4,7]],"date-time":"2017-04-07T11:01:03Z","timestamp":1491562863000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":121,"title":["Targeting microbial biofilms using Ficin, a nonspecific plant protease"],"prefix":"10.1038","volume":"7","author":[{"given":"Diana R.","family":"Baidamshina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena Y.","family":"Trizna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina G.","family":"Holyavka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikhail I.","family":"Bogachev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeriy G.","family":"Artyukhov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farida S.","family":"Akhatova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elvira V.","family":"Rozhina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rawil F.","family":"Fakhrullin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Airat R.","family":"Kayumov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,4,7]]},"reference":[{"key":"BFsrep46068_CR1","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1111\/j.1574-695X.2011.00858.x","volume":"65","author":"L Yang","year":"2012","unstructured":"Yang, L. et al. 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