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To characterize SM disease in chickens, the pathogenic traits and tissue predilections of the disease were investigated. Dissemination of fluorescent-tagged SM (JOL1575GFP) was monitored after oral and intramuscular mock infections of specific-pathogen-free chickens. The spleen was predominantly affected by intramuscular infection while the cecum, spleen, and minimally liver were affected by oral infection. No conspicuous illness was observed in infected birds, and histopathological examination showed minimal damage of the intestinal epithelium and splenic parenchyma though SM was readily isolated from these tissues. Levels of SM internalization by primary chicken peritoneal macrophages were similar to that of <jats:italic>Salmonella<\/jats:italic> Typhimurium. SM was more sensitive to chicken than rabbit serum complement killing. Internal egg contamination of SM mock infected layers also occurred at trace levels and lasted for a week after inoculation. This study also confirmed that SM infection in chickens is sub-clinical and asymptomatic, which suggests that latent asymptomatic carriers may excrete a large number of bacteria and transmit the pathogen by contaminating water or food sources.<\/jats:p>","DOI":"10.1038\/srep46232","type":"journal-article","created":{"date-parts":[[2017,4,7]],"date-time":"2017-04-07T11:17:08Z","timestamp":1491563828000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Pathogenic traits of Salmonella Montevideo in experimental infections in vivo and in vitro"],"prefix":"10.1038","volume":"7","author":[{"given":"Jonathan","family":"Lalsiamthara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John Hwa","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2017,4,7]]},"reference":[{"key":"BFsrep46232_CR1","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1038\/sj.icb.7100007","volume":"85","author":"B Coburn","year":"2007","unstructured":"Coburn, B., Grassl, G. 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The error has not been fixed in the paper.","order":6,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 May 2017","order":7,"name":"change_date","label":"Change Date","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Erratum","order":8,"name":"change_type","label":"Change Type","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"Scientific Reports 7: Article number: 46232; published online: 07 April 2017; updated: 04 May 2017 The Acknowledgements section in this Article has been omitted. It should read: \u201cAcknowledgements This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MISP) (No.","order":9,"name":"change_details","label":"Change Details","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"46232"}}