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Pyrethroids remain one of the most used insecticides to control<jats:italic>Aedes<\/jats:italic>mosquitoes, despite the development of pyrethroid resistance in many mosquito populations worldwide. Here, we report a Brazilian strain of<jats:italic>A. aegypti<\/jats:italic>with high levels (approximately 100\u201360,000 fold) of resistance to both type I and type II pyrethroids. We detected two mutations (V410L and F1534C) in the sodium channel from this resistant strain. This study is the first report of the V410L mutation in mosquitoes. Alone or in combination with the F1534C mutation, the V410L mutation drastically reduced the sensitivity of mosquito sodium channels expressed in<jats:italic>Xenopus<\/jats:italic>oocytes to both type I and type II pyrethroids. The V410L mutation presents a serious challenge for the control of<jats:italic>A. aegypti<\/jats:italic>and will compromise the use of pyrethroids for the control of<jats:italic>A. aegypti<\/jats:italic>in Brazil; therefore, early monitoring of the frequency of the V410L mutation will be a key resistance management strategy to preserve the effectiveness of pyrethroid insecticides.<\/jats:p>","DOI":"10.1038\/srep46549","type":"journal-article","created":{"date-parts":[[2017,4,19]],"date-time":"2017-04-19T11:41:16Z","timestamp":1492602076000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":199,"title":["Detection of a new pyrethroid resistance mutation (V410L) in the sodium channel of Aedes aegypti: a potential challenge for mosquito control"],"prefix":"10.1038","volume":"7","author":[{"given":"Khalid","family":"Haddi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hudson V. 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