{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T08:26:22Z","timestamp":1776155182908,"version":"3.50.1"},"reference-count":48,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2004,5,16]],"date-time":"2004-05-16T00:00:00Z","timestamp":1084665600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nat Biotechnol"],"published-print":{"date-parts":[[2004,6]]},"DOI":"10.1038\/nbt969","type":"journal-article","created":{"date-parts":[[2004,5,16]],"date-time":"2004-05-16T17:06:24Z","timestamp":1084727184000},"page":"724-731","source":"Crossref","is-referenced-by-count":139,"title":["Time-controlled transcardiac perfusion cross-linking for the study of protein interactions in complex tissues"],"prefix":"10.1038","volume":"22","author":[{"given":"Gerold","family":"Schmitt-Ulms","sequence":"first","affiliation":[]},{"given":"Kirk","family":"Hansen","sequence":"additional","affiliation":[]},{"given":"Jialing","family":"Liu","sequence":"additional","affiliation":[]},{"given":"Cynthia","family":"Cowdrey","sequence":"additional","affiliation":[]},{"given":"Jian","family":"Yang","sequence":"additional","affiliation":[]},{"given":"Stephen J","family":"DeArmond","sequence":"additional","affiliation":[]},{"given":"Fred E","family":"Cohen","sequence":"additional","affiliation":[]},{"given":"Stanley B","family":"Prusiner","sequence":"additional","affiliation":[]},{"given":"Michael A","family":"Baldwin","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2004,5,16]]},"reference":[{"key":"BFnbt969_CR1","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1002\/1615-9861(200206)2:6<611::AID-PROT611>3.0.CO;2-Y","volume":"2","author":"D Auerbach","year":"2002","unstructured":"Auerbach, D., Thaminy, S., Hottiger, M.O. & Stagljar, I. The post-genomic era of interactive proteomics: facts and perspectives. Proteomics 2, 611\u2013623 (2002).","journal-title":"Proteomics"},{"key":"BFnbt969_CR2","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1016\/S0167-7799(01)01811-X","volume":"19","author":"T Reiss","year":"2001","unstructured":"Reiss, T. Drug discovery of the future: the implications of the human genome project. Trends Biotechnol. 19, 496\u2013499 (2001).","journal-title":"Trends Biotechnol."},{"key":"BFnbt969_CR3","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1152\/physrev.2001.81.2.741","volume":"81","author":"DJ Selkoe","year":"2001","unstructured":"Selkoe, D.J. Alzheimer's disease: genes, proteins, and therapy. Physiol. Rev. 81, 741\u2013766 (2001).","journal-title":"Physiol. Rev."},{"key":"BFnbt969_CR4","doi-asserted-by":"publisher","first-page":"556","DOI":"10.1016\/S0962-8924(02)02394-2","volume":"12","author":"C Haass","year":"2002","unstructured":"Haass, C. & Steiner, H. Alzheimer disease gamma-secretase: a complex story of GxGD-type presenilin proteases. Trends Cell Biol. 12, 556\u2013562 (2002).","journal-title":"Trends Cell Biol."},{"key":"BFnbt969_CR5","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1038\/376775a0","volume":"376","author":"EI Rogaev","year":"1995","unstructured":"Rogaev, E.I. et al. Familial Alzheimer's disease in kindreds with missense mutations in a gene on chromosome 1 related to the Alzheimer's disease type 3 gene. Nature 376, 775\u2013778 (1995).","journal-title":"Nature"},{"key":"BFnbt969_CR6","doi-asserted-by":"publisher","first-page":"754","DOI":"10.1038\/375754a0","volume":"375","author":"R Sherrington","year":"1995","unstructured":"Sherrington, R. et al. Cloning of a gene bearing missense mutations in early-onset familial Alzheimer's disease. Nature 375, 754\u2013760 (1995).","journal-title":"Nature"},{"key":"BFnbt969_CR7","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1038\/35024009","volume":"407","author":"G Yu","year":"2000","unstructured":"Yu, G. et al. Nicastrin modulates presenilin-mediated notch\/glp-1 signal transduction and \u03b2APP processing. Nature 407, 48\u201354 (2000).","journal-title":"Nature"},{"key":"BFnbt969_CR8","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1073\/pnas.022523499","volume":"99","author":"C Goutte","year":"2002","unstructured":"Goutte, C., Tsunozaki, M., Hale, V.A. & Priess, J.R. APH-1 is a multipass membrane protein essential for the Notch signaling pathway in Caenorhabditis elegans embryos. Proc. Natl. Acad. Sci. USA 99, 775\u2013779 (2002).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnbt969_CR9","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/S1534-5807(02)00189-2","volume":"3","author":"R Francis","year":"2002","unstructured":"Francis, R. et al. aph-1 and pen-2 are required for Notch pathway signaling, gamma-secretase cleavage of betaAPP, and presenilin protein accumulation. Dev. Cell 3, 85\u201397 (2002).","journal-title":"Dev. Cell"},{"key":"BFnbt969_CR10","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1083\/jcb.129.3.619","volume":"129","author":"A Gorodinsky","year":"1995","unstructured":"Gorodinsky, A. & Harris, D.A. Glycolipid-anchored proteins in neuroblastoma cells form detergent-resistant complexes without caveolin. J. Cell Biol. 129, 619\u2013627 (1995).","journal-title":"J. Cell Biol."},{"key":"BFnbt969_CR11","doi-asserted-by":"publisher","first-page":"6324","DOI":"10.1074\/jbc.272.10.6324","volume":"272","author":"N Naslavsky","year":"1997","unstructured":"Naslavsky, N., Stein, R., Yanai, A., Friedlander, G. & Taraboulos, A. Characterization of detergent-insoluble complexes containing the cellular prion protein and its scrapie isoform. J. Biol. Chem. 272, 6324\u20136331 (1997).","journal-title":"J. Biol. Chem."},{"key":"BFnbt969_CR12","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1083\/jcb.129.1.121","volume":"129","author":"A Taraboulos","year":"1995","unstructured":"Taraboulos, A. et al. Cholesterol depletion and modification of COOH-terminal targeting sequence of the prion protein inhibits formation of the scrapie isoform. J. Cell Biol. 129, 121\u2013132 (1995).","journal-title":"J. Cell Biol."},{"key":"BFnbt969_CR13","doi-asserted-by":"publisher","first-page":"14945","DOI":"10.1073\/pnas.93.25.14945","volume":"93","author":"M Vey","year":"1996","unstructured":"Vey, M. et al. Subcellular colocalization of the cellular and scrapie prion proteins in caveolae-like membranous domains. Proc. Natl. Acad. Sci. USA 93, 14945\u201314949 (1996).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnbt969_CR14","doi-asserted-by":"publisher","first-page":"2333","DOI":"10.1073\/pnas.94.6.2333","volume":"94","author":"K Kaneko","year":"1997","unstructured":"Kaneko, K. et al. COOH-terminal sequence of the cellular prion protein directs subcellular trafficking and controls conversion into the scrapie isoform. Proc. Natl. Acad. Sci. USA 94, 2333\u20132338 (1997).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnbt969_CR15","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/S0092-8674(03)00201-0","volume":"113","author":"P Meier","year":"2003","unstructured":"Meier, P. et al. Soluble dimeric prion protein binds PrPScin vivo and antagonizes prion disease. Cell 113, 49\u201360 (2003).","journal-title":"Cell"},{"key":"BFnbt969_CR16","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/S1046-2023(02)00007-5","volume":"26","author":"J Wells","year":"2002","unstructured":"Wells, J. & Farnham, P.J. Characterizing transcription factor binding sites using formaldehyde crosslinking and immunoprecipitation. Methods 26, 48\u201356 (2002).","journal-title":"Methods"},{"key":"BFnbt969_CR17","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1006\/meth.1998.0724","volume":"17","author":"V Jackson","year":"1999","unstructured":"Jackson, V. Formaldehyde cross-linking for studying nucleosomal dynamics. Methods 17, 125\u2013139 (1999).","journal-title":"Methods"},{"key":"BFnbt969_CR18","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1006\/meth.1996.0407","volume":"11","author":"V Orlando","year":"1997","unstructured":"Orlando, V., Strutt, H. & Paro, R. Analysis of chromatin structure by in vivo formaldehyde cross-linking. Methods 11, 205\u2013214 (1997).","journal-title":"Methods"},{"key":"BFnbt969_CR19","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1006\/meth.1996.0411","volume":"11","author":"G Fragoso","year":"1997","unstructured":"Fragoso, G. & Hager, G.L. Analysis of in vivo nucleosome positions by determination of nucleosome-linker boundaries in crosslinked chromatin. Methods 11, 246\u2013252 (1997).","journal-title":"Methods"},{"key":"BFnbt969_CR20","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1006\/meth.1998.0664","volume":"16","author":"MJ Hannah","year":"1998","unstructured":"Hannah, M.J., Weiss, U. & Huttner, W.B. Differential extraction of proteins from paraformaldehyde-fixed cells: lessons from Synaptophysin and other membrane proteins. Methods 16, 170\u2013181 (1998).","journal-title":"Methods"},{"key":"BFnbt969_CR21","doi-asserted-by":"publisher","first-page":"1209","DOI":"10.1006\/jmbi.2000.5183","volume":"314","author":"G Schmitt-Ulms","year":"2001","unstructured":"Schmitt-Ulms, G. et al. Binding of neural cell adhesion molecules (N-CAMs) to the cellular prion protein. J. Mol. Biol. 314, 1209\u20131225 (2001).","journal-title":"J. Mol. Biol."},{"key":"BFnbt969_CR22","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1038\/ncb960","volume":"5","author":"D Edbauer","year":"2003","unstructured":"Edbauer, D. et al. Reconstitution of gamma-secretase activity. Nat. Cell Biol. 5, 486\u2013488 (2003).","journal-title":"Nat. Cell Biol."},{"key":"BFnbt969_CR23","doi-asserted-by":"publisher","first-page":"577","DOI":"10.1038\/356577a0","volume":"356","author":"H B\u00fceler","year":"1992","unstructured":"B\u00fceler, H. et al. Normal development and behaviour of mice lacking the neuronal cell-surface PrP protein. Nature 356, 577\u2013582 (1992).","journal-title":"Nature"},{"key":"BFnbt969_CR24","doi-asserted-by":"publisher","first-page":"2597","DOI":"10.1073\/pnas.95.5.2597","volume":"95","author":"BD Holst","year":"1998","unstructured":"Holst, B.D. et al. Allosteric modulation of AMPA-type glutamate receptors increases activity of the promoter for the neural cell adhesion molecule, N-CAM. Proc. Natl. Acad. Sci. USA 95, 2597\u20132602 (1998).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnbt969_CR25","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1016\/S1044-7431(02)00014-3","volume":"22","author":"S Chen","year":"2003","unstructured":"Chen, S., Mange, A., Dong, L., Lehmann, S. & Schachner, M. Prion protein as trans-interacting partner for neurons is involved in neurite outgrowth and neuronal survival. Mol. Cell. Neurosci. 22, 227\u2013233 (2003).","journal-title":"Mol. Cell. Neurosci."},{"key":"BFnbt969_CR26","doi-asserted-by":"publisher","first-page":"16470","DOI":"10.1074\/jbc.273.26.16470","volume":"273","author":"G Yu","year":"1998","unstructured":"Yu, G. et al. The presenilin 1 protein is a component of a high molecular weight intracellular complex that contains beta-catenin. J. Biol. Chem. 273, 16470\u201316475 (1998).","journal-title":"J. Biol. Chem."},{"key":"BFnbt969_CR27","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1016\/S1044-7431(02)00031-3","volume":"22","author":"M Vinson","year":"2003","unstructured":"Vinson, M. et al. Lipid rafts mediate the interaction between myelin-associated glycoprotein (MAG) on myelin and MAG-receptors on neurons. Mol. Cell. Neurosci. 22, 344\u2013352 (2003).","journal-title":"Mol. Cell. Neurosci."},{"key":"BFnbt969_CR28","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1083\/jcb.200207113","volume":"160","author":"R Ehehalt","year":"2003","unstructured":"Ehehalt, R., Keller, P., Haass, C., Thiele, C. & Simons, K. Amyloidogenic processing of the Alzheimer beta-amyloid precursor protein depends on lipid rafts. J. Cell Biol. 160, 113\u2013123 (2003).","journal-title":"J. Cell Biol."},{"key":"BFnbt969_CR29","doi-asserted-by":"publisher","first-page":"38699","DOI":"10.1074\/jbc.M005543200","volume":"275","author":"T Jin","year":"2000","unstructured":"Jin, T. et al. The chaperone protein BiP binds to a mutant prion protein and mediates its degradation by the proteasome. J. Biol. Chem. 275, 38699\u201338704 (2000).","journal-title":"J. Biol. Chem."},{"key":"BFnbt969_CR30","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1006\/nbdi.1997.0130","volume":"3","author":"F Yehiely","year":"1997","unstructured":"Yehiely, F. et al. Identification of candidate proteins binding to prion protein. Neurobiol. Dis. 3, 339\u2013355 (1997).","journal-title":"Neurobiol. Dis."},{"key":"BFnbt969_CR31","doi-asserted-by":"publisher","first-page":"37743","DOI":"10.1074\/jbc.M003965200","volume":"276","author":"B Vincent","year":"2001","unstructured":"Vincent, B. et al. The disintegrins ADAM10 and TACE contribute to the constitutive and phorbol ester-regulated normal cleavage of the cellular prion protein. J. Biol. Chem. 276, 37743\u201337746 (2001).","journal-title":"J. Biol. Chem."},{"key":"BFnbt969_CR32","doi-asserted-by":"publisher","first-page":"30853","DOI":"10.1074\/jbc.270.52.30853","volume":"270","author":"F Fiore","year":"1995","unstructured":"Fiore, F. et al. The regions of the Fe65 protein homologous to the phosphotyrosine interaction\/phosphotyrosine binding domain of Shc bind the intracellular domain of the Alzheimer's amyloid precursor protein. J. Biol. Chem. 270, 30853\u201330856 (1995).","journal-title":"J. Biol. Chem."},{"key":"BFnbt969_CR33","doi-asserted-by":"publisher","first-page":"2307","DOI":"10.1046\/j.1471-4159.1995.65052307.x","volume":"65","author":"S Olive","year":"1995","unstructured":"Olive, S., Dubois, C., Schachner, M. & Rougon, G. The F3 neuronal glycosylphosphatidylinositol-linked molecule is localized to glycolipid-enriched membrane subdomains and interacts with L1 and fyn kinase in cerebellum. J. Neurochem. 65, 2307\u20132317 (1995).","journal-title":"J. Neurochem."},{"key":"BFnbt969_CR34","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1016\/S0092-8674(03)00882-1","volume":"115","author":"S Munro","year":"2003","unstructured":"Munro, S. Lipid rafts: elusive or illusive? Cell 115, 377\u2013388 (2003).","journal-title":"Cell"},{"key":"BFnbt969_CR35","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1016\/0092-8674(92)90189-J","volume":"68","author":"DA Brown","year":"1992","unstructured":"Brown, D.A. & Rose, J.K. Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface. Cell 68, 533\u2013544 (1992).","journal-title":"Cell"},{"key":"BFnbt969_CR36","doi-asserted-by":"publisher","first-page":"1010","DOI":"10.1002\/1615-9861(200108)1:8<1010::AID-PROT1010>3.0.CO;2-L","volume":"1","author":"PD von Haller","year":"2001","unstructured":"von Haller, P.D., Donohoe, S., Goodlett, D.R., Aebersold, R. & Watts, J.D. Mass spectrometric characterization of proteins extracted from Jurkat T cell detergent-resistant membrane domains. Proteomics 1, 1010\u20131021 (2001).","journal-title":"Proteomics"},{"key":"BFnbt969_CR37","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1042\/bj20020503","volume":"369","author":"L Bini","year":"2003","unstructured":"Bini, L. et al. Extensive temporally regulated reorganization of the lipid raft proteome following T-cell antigen receptor triggering. Biochem. J. 369, 301\u2013309 (2003).","journal-title":"Biochem. J."},{"key":"BFnbt969_CR38","doi-asserted-by":"publisher","first-page":"5813","DOI":"10.1073\/pnas.0631608100","volume":"100","author":"LJ Foster","year":"2003","unstructured":"Foster, L.J., De Hoog, C.L. & Mann, M. Unbiased quantitative proteomics of lipid rafts reveals high specificity for signaling factors. Proc. Natl. Acad. Sci. USA 100, 5813\u20135818 (2003).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnbt969_CR39","doi-asserted-by":"publisher","first-page":"6917","DOI":"10.1093\/emboj\/18.24.6917","volume":"18","author":"N Madore","year":"1999","unstructured":"Madore, N. et al. Functionally different GPI proteins are organized in different domains on the neuronal surface. EMBO J. 18, 6917\u20136926 (1999).","journal-title":"EMBO J."},{"key":"BFnbt969_CR40","doi-asserted-by":"publisher","first-page":"7640","DOI":"10.1074\/jbc.271.13.7640","volume":"271","author":"C Bouillot","year":"1996","unstructured":"Bouillot, C., Prochiantz, A., Rougon, G. & Allinquant, B. Axonal amyloid precursor protein expressed by neurons in vitro is present in a membrane fraction with caveolae-like properties. J. Biol. Chem. 271, 7640\u20137644 (1996).","journal-title":"J. Biol. Chem."},{"key":"BFnbt969_CR41","doi-asserted-by":"publisher","first-page":"10485","DOI":"10.1074\/jbc.273.17.10485","volume":"273","author":"T Ikezu","year":"1998","unstructured":"Ikezu, T. et al. Caveolae, plasma membrane microdomains for alpha-secretase-mediated processing of the amyloid precursor protein. J. Biol. Chem. 273, 10485\u201310495 (1998).","journal-title":"J. Biol. Chem."},{"key":"BFnbt969_CR42","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1074\/jbc.M304675200","volume":"279","author":"W Wong","year":"2004","unstructured":"Wong, W. & Schlichter, L.C. Differential recruitment of Kv1.4 and Kv4.2 to lipid rafts by PSD-95. J. Biol. Chem. 279, 444\u2013452 (2004).","journal-title":"J. Biol. Chem."},{"key":"BFnbt969_CR43","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1016\/S0896-6273(02)01185-6","volume":"37","author":"MS Nadal","year":"2003","unstructured":"Nadal, M.S. et al. The CD26-related dipeptidyl aminopeptidase-like protein DPPX is a critical component of neuronal A-type K+ channels. Neuron 37, 449\u2013461 (2003).","journal-title":"Neuron"},{"key":"BFnbt969_CR44","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1126\/science.1069784","volume":"296","author":"L Pelkmans","year":"2002","unstructured":"Pelkmans, L., Puntener, D. & Helenius, A. Local actin polymerization and dynamin recruitment in SV40-induced internalization of caveolae. Science 296, 535\u2013539 (2002).","journal-title":"Science"},{"key":"BFnbt969_CR45","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1083\/jcb.200302028","volume":"161","author":"IR Nabi","year":"2003","unstructured":"Nabi, I.R. & Le, P.U. Caveolae\/raft-dependent endocytosis. J. Cell Biol. 161, 673\u2013677 (2003).","journal-title":"J. Cell Biol."},{"key":"BFnbt969_CR46","doi-asserted-by":"publisher","first-page":"1408","DOI":"10.1128\/JVI.75.3.1408-1413.2001","volume":"75","author":"S Supattapone","year":"2001","unstructured":"Supattapone, S. et al. Identification of two prion protein regions that modify scrapie incubation time. J. Virol. 75, 1408\u20131413 (2001).","journal-title":"J. Virol."},{"key":"BFnbt969_CR47","doi-asserted-by":"crossref","first-page":"9413","DOI":"10.1128\/JVI.72.11.9413-9418.1998","volume":"72","author":"RA Williamson","year":"1998","unstructured":"Williamson, R.A. et al. Mapping the prion protein using recombinant antibodies. J. Virol. 72, 9413\u20139418 (1998).","journal-title":"J. Virol."},{"key":"BFnbt969_CR48","doi-asserted-by":"publisher","first-page":"2871","DOI":"10.1021\/ac9810516","volume":"71","author":"KR Clauser","year":"1999","unstructured":"Clauser, K.R., Baker, P. & Burlingame, A.L. Role of accurate mass measurement (\u00b110 ppm) in protein identification strategies employing MS or MS\/MS and database searching. Anal. Chem. 71, 2871\u20132882 (1999).","journal-title":"Anal. Chem."}],"container-title":["Nature Biotechnology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nbt969.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nbt969","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nbt969.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T19:58:06Z","timestamp":1684439886000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nbt969"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,5,16]]},"references-count":48,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2004,6]]}},"alternative-id":["BFnbt969"],"URL":"https:\/\/doi.org\/10.1038\/nbt969","relation":{},"ISSN":["1087-0156","1546-1696"],"issn-type":[{"value":"1087-0156","type":"print"},{"value":"1546-1696","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,5,16]]}}}