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In yeast and plant cells, a key detoxifying mechanism involves iron sequestration into intracellular storage compartments, mediated by members of the vacuolar iron-transporter (VIT) family of proteins. Here we study the VIT homologue from the malaria parasites <jats:italic>Plasmodium falciparum<\/jats:italic> (PfVIT) and <jats:italic>Plasmodium berghei<\/jats:italic> (PbVIT). PfVIT-mediated iron transport in a yeast heterologous expression system is saturable (<jats:italic>K<\/jats:italic><jats:sub>m<\/jats:sub>\u223c14.7\u2009\u03bcM), and selective for Fe<jats:sup>2+<\/jats:sup> over other divalent cations. PbVIT-deficient <jats:italic>P. berghei<\/jats:italic> lines (<jats:italic>Pbvit<\/jats:italic><jats:sup>\u2212<\/jats:sup>) show a reduction in parasite load in both liver and blood stages of infection in mice. Moreover, <jats:italic>Pbvit<\/jats:italic><jats:sup>\u2212<\/jats:sup> parasites have higher levels of labile iron in blood stages and are more sensitive to increased iron levels in liver stages, when compared with wild-type parasites. Our data are consistent with <jats:italic>Plasmodium<\/jats:italic> VITs playing a major role in iron detoxification and, thus, normal development of malaria parasites in their mammalian host.<\/jats:p>","DOI":"10.1038\/ncomms10403","type":"journal-article","created":{"date-parts":[[2016,1,20]],"date-time":"2016-01-20T11:21:31Z","timestamp":1453288891000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["A vacuolar iron-transporter homologue acts as a detoxifier in Plasmodium"],"prefix":"10.1038","volume":"7","author":[{"given":"Ksenija","family":"Slavic","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0066-0634","authenticated-orcid":false,"given":"Sanjeev","family":"Krishna","sequence":"additional","affiliation":[]},{"given":"Aparajita","family":"Lahree","sequence":"additional","affiliation":[]},{"given":"Guillaume","family":"Bouyer","sequence":"additional","affiliation":[]},{"given":"Kirsten K.","family":"Hanson","sequence":"additional","affiliation":[]},{"given":"Iset","family":"Vera","sequence":"additional","affiliation":[]},{"given":"Jon K.","family":"Pittman","sequence":"additional","affiliation":[]},{"given":"Henry M.","family":"Staines","sequence":"additional","affiliation":[]},{"given":"Maria M.","family":"Mota","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2016,1,20]]},"reference":[{"key":"BFncomms10403_CR1","unstructured":"WHO. 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