{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T18:22:51Z","timestamp":1771266171815,"version":"3.50.1"},"reference-count":34,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2005,2,17]],"date-time":"2005-02-17T00:00:00Z","timestamp":1108598400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nat Methods"],"published-print":{"date-parts":[[2005,3]]},"DOI":"10.1038\/nmeth738","type":"journal-article","created":{"date-parts":[[2005,2,17]],"date-time":"2005-02-17T22:54:49Z","timestamp":1108680889000},"page":"193-200","source":"Crossref","is-referenced-by-count":41,"title":["A new method for C-terminal sequence analysis in the proteomic era"],"prefix":"10.1038","volume":"2","author":[{"given":"Bart","family":"Samyn","sequence":"first","affiliation":[]},{"given":"Kjell","family":"Sergeant","sequence":"additional","affiliation":[]},{"given":"Pedro","family":"Castanheira","sequence":"additional","affiliation":[]},{"given":"Carlos","family":"Faro","sequence":"additional","affiliation":[]},{"given":"Jozef","family":"Van Beeumen","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2005,2,17]]},"reference":[{"key":"BFnmeth738_CR1","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1016\/S0968-0004(01)02021-7","volume":"27","author":"J Rappsilber","year":"2002","unstructured":"Rappsilber, J. & Mann, M. What does it mean to identify a protein in proteomics? Trends Biochem. Sci. 27, 74\u201378 (2002).","journal-title":"Trends Biochem. Sci."},{"key":"BFnmeth738_CR2","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1038\/nbt0303-255","volume":"21","author":"M Mann","year":"2003","unstructured":"Mann, M. & Jensen, O.N. Proteomic analysis of post-translational modifications. Nat. Biotechnol. 21, 255\u2013261 (2003).","journal-title":"Nat. Biotechnol."},{"key":"BFnmeth738_CR3","doi-asserted-by":"publisher","first-page":"1047","DOI":"10.1038\/nbt863","volume":"21","author":"ZA Knight","year":"2003","unstructured":"Knight, Z.A. et al. Phosphospecific proteolysis for mapping sites of protein phosphorylation. Nat. Biotechnol. 21, 1047\u20131054 (2003).","journal-title":"Nat. Biotechnol."},{"key":"BFnmeth738_CR4","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1038\/86777","volume":"19","author":"H Zhou","year":"2001","unstructured":"Zhou, H., Watts, J.D. & Aebersold, R. A systematic approach to the analysis of protein phosphorylation. Nat. Biotechnol. 19, 375\u2013378 (2001).","journal-title":"Nat. Biotechnol."},{"key":"BFnmeth738_CR5","doi-asserted-by":"publisher","first-page":"913","DOI":"10.1038\/nbt1001-913","volume":"19","author":"A Dove","year":"2001","unstructured":"Dove, A. The bittersweet promise of glycobiology. Nat. Biotechnol. 19, 913\u2013917 (2001).","journal-title":"Nat. Biotechnol."},{"key":"BFnmeth738_CR6","doi-asserted-by":"publisher","first-page":"1622","DOI":"10.1002\/pro.5560011210","volume":"1","author":"JM Bailey","year":"1992","unstructured":"Bailey, J.M., Nikfarjam, F., Shenoy, N.R. & Shively, J.E. Automated carboxyl-terminal sequence analysis of peptides and proteins using diphenyl phosphoroisothiocyanatidate. Protein Sci. 1, 1622\u20131633 (1992).","journal-title":"Protein Sci."},{"key":"BFnmeth738_CR7","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1016\/0003-2697(92)90376-I","volume":"206","author":"VL Boyd","year":"1992","unstructured":"Boyd, V.L., Bozzini, M., Zon, G., Noble, R.L. & Mattaliano, R.J. Sequencing of peptides and proteins from the carboxy terminus. Anal. Biochem. 206, 344\u2013352 (1992).","journal-title":"Anal. Biochem."},{"key":"BFnmeth738_CR8","doi-asserted-by":"publisher","first-page":"1389","DOI":"10.1021\/ac991049u","volume":"72","author":"B Samyn","year":"2000","unstructured":"Samyn, B., Hardeman, K., Van der Eycken, J. & Van Beeumen, J. Applicability of the alkylation chemistry for chemical C-terminal protein sequence analysis. Anal. Chem. 72, 1389\u20131399 (2000).","journal-title":"Anal. Chem."},{"key":"BFnmeth738_CR9","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/0021-9673(94)01250-I","volume":"705","author":"JM Bailey","year":"1995","unstructured":"Bailey, J.M. Chemical methods of protein sequence analysis. J. Chromat. A 705, 47\u201365 (1995).","journal-title":"J. Chromat. A"},{"key":"BFnmeth738_CR10","doi-asserted-by":"publisher","first-page":"1593","DOI":"10.1002\/pro.5560070713","volume":"7","author":"K Hardeman","year":"1998","unstructured":"Hardeman, K., Samyn, B., Van der Eycken, J. & Van Beeumen, J. An improved chemical approach toward the C-terminal sequence analysis of proteins containing all natural amino acids. Protein Sci. 7, 1593\u20131602 (1998).","journal-title":"Protein Sci."},{"key":"BFnmeth738_CR11","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1038\/nature01511","volume":"422","author":"R Aebersold","year":"2003","unstructured":"Aebersold, R. & Mann, M. Mass spectrometry-based proteomics. Nature 422, 198\u2013207 (2003).","journal-title":"Nature"},{"key":"BFnmeth738_CR12","doi-asserted-by":"publisher","first-page":"952","DOI":"10.1038\/nbt1001-952","volume":"19","author":"F Meng","year":"2001","unstructured":"Meng, F. et al. Informatics and multiplexing of intact protein identification in bacteria and the archaea. Nat. Biotechnol. 19, 952\u2013957 (2001).","journal-title":"Nat. Biotechnol."},{"key":"BFnmeth738_CR13","doi-asserted-by":"publisher","first-page":"2923","DOI":"10.1021\/ac020049i","volume":"74","author":"F Meng","year":"2002","unstructured":"Meng, F. et al. Processing complex mixtures of intact proteins for direct analysis by mass spectrometry. Anal. Chem. 74, 2923\u20132929 (2002).","journal-title":"Anal. Chem."},{"key":"BFnmeth738_CR14","doi-asserted-by":"publisher","first-page":"5817","DOI":"10.1021\/ac034362b","volume":"75","author":"D Suckau","year":"2003","unstructured":"Suckau, D. & Resemann, A. Targeted characterization of the N- and C-termini of undigested proteins by mass spectrometry. Anal. Chem. 75, 5817\u20135824 (2003).","journal-title":"Anal. Chem."},{"key":"BFnmeth738_CR15","doi-asserted-by":"publisher","first-page":"3374","DOI":"10.1021\/ac000045i","volume":"72","author":"S Sechi","year":"2000","unstructured":"Sechi, S. & Chait, B.T. A method to define the carboxyl terminal of proteins. Anal. Chem. 72, 3374\u20133378 (2000).","journal-title":"Anal. Chem."},{"key":"BFnmeth738_CR16","doi-asserted-by":"publisher","first-page":"1179","DOI":"10.1021\/ac991067b","volume":"72","author":"T Kosaka","year":"2000","unstructured":"Kosaka, T., Takazawa, T. & Nakamura, T. Identification and C-terminal characterization of proteins from two-dimensional polyacrylamide gels by a combination of isotopic labeling and nano-electrospray Fourier transform ion cyclotron resonance mass spectrometry. Anal. Chem. 72, 1179\u20131185 (2000).","journal-title":"Anal. Chem."},{"key":"BFnmeth738_CR17","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1074\/mcp.M300123-MCP200","volume":"3","author":"XW Zhou","year":"2004","unstructured":"Zhou, X.W., Blackman, M.J., Howell, S.A. & Carruthers, V.B. Proteomic analysis of cleavage events reveals a dynamic two-step mechanism for proteolysis of a key parasite adhesive complex. Mol. Cell. Proteomics 3, 565\u2013576 (2004).","journal-title":"Mol. Cell. Proteomics"},{"key":"BFnmeth738_CR18","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1126\/science.8211132","volume":"262","author":"BT Chait","year":"1993","unstructured":"Chait, B.T., Wang, R., Beavis, R.C. & Kent, S.B.H. Protein ladder sequencing. Science 262, 89\u201392 (1993).","journal-title":"Science"},{"key":"BFnmeth738_CR19","doi-asserted-by":"publisher","first-page":"1644","DOI":"10.1021\/ac00105a027","volume":"67","author":"CM Murphy","year":"1995","unstructured":"Murphy, C.M. & Fenselau, C. Recognition of the carboxy-terminal peptide in cyanogen bromide digests of proteins. Anal. Chem. 67, 1644\u20131645 (1995).","journal-title":"Anal. Chem."},{"key":"BFnmeth738_CR20","doi-asserted-by":"publisher","first-page":"3971","DOI":"10.1021\/ac00117a024","volume":"67","author":"DH Patterson","year":"1995","unstructured":"Patterson, D.H., Tarr, G.E., Regnier, F.E. & Martin, S.A. C-terminal ladder sequencing via matrix-assisted laser desorption mass spectrometry coupled with carboxypeptidase Y time dependent and concentration-dependent digestions. Anal. Chem. 67, 3971\u20133978 (1995).","journal-title":"Anal. Chem."},{"key":"BFnmeth738_CR21","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1126\/science.1066577","volume":"295","author":"S Glasauer","year":"2002","unstructured":"Glasauer, S., Langley, S. & Beveridge, T.J. Intracellular iron minerals in a dissimilatory iron-reducing bacterium. Science 295, 117\u2013119 (2002).","journal-title":"Science"},{"key":"BFnmeth738_CR22","doi-asserted-by":"publisher","first-page":"2249","DOI":"10.1002\/pmic.200300476","volume":"3","author":"F Vanrobaeys","year":"2003","unstructured":"Vanrobaeys, F. et al. Proteomics of the dissimilatory iron-reducing bacterium Shewanella oneidensis MR-1, using a matrix-assisted laser desorption\/ionization-tandem-time of flight mass spectrometer. Proteomics 3, 2249\u20132257 (2003).","journal-title":"Proteomics"},{"key":"BFnmeth738_CR23","doi-asserted-by":"publisher","first-page":"3551","DOI":"10.1002\/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2","volume":"20","author":"DN Perkins","year":"1999","unstructured":"Perkins, D.N., Pappin, D.J., Creasy, D.M. & Cottrell, J.S. Probability-based identification by searching sequence databases using mass spectrometry data. Electrophoresis 20, 3551\u20133567 (1999).","journal-title":"Electrophoresis"},{"key":"BFnmeth738_CR24","doi-asserted-by":"publisher","first-page":"2067","DOI":"10.1111\/j.1432-1033.2004.04136.x","volume":"271","author":"I Sim\u00f5es","year":"2004","unstructured":"Sim\u00f5es, I. & Faro, C. Structure and function of plant aspartic proteinases. Eur. J. Biochem. 271, 2067\u20132075 (2004).","journal-title":"Eur. J. Biochem."},{"key":"BFnmeth738_CR25","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1046\/j.1432-1327.1998.2550133.x","volume":"255","author":"M Ramalho-Santos","year":"1998","unstructured":"Ramalho-Santos, M. et al. Identification and proteolytic processing of procardosin A. Eur. J. Biochem. 255, 133\u2013138 (1998).","journal-title":"Eur. J. Biochem."},{"key":"BFnmeth738_CR26","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1038\/86783","volume":"19","author":"Y Oda","year":"2001","unstructured":"Oda, Y., Nagasu, T. & Chait, B.T. Enrichment analysis of phosphorylated proteins as a tool for probing the proteome. Nat. Biotechnol. 19, 379\u2013382 (2001).","journal-title":"Nat. Biotechnol."},{"key":"BFnmeth738_CR27","doi-asserted-by":"publisher","first-page":"1340","DOI":"10.1021\/ac026213j","volume":"75","author":"JJ Jones","year":"2003","unstructured":"Jones, J.J. et al. Investigation of MALDI-TOF and FT-MS techniques for analysis of Escherichia coli whole cells. Anal. Chem. 75, 1340\u20131347 (2003).","journal-title":"Anal. Chem."},{"key":"BFnmeth738_CR28","doi-asserted-by":"publisher","first-page":"242","DOI":"10.1038\/85686","volume":"19","author":"MP Washburn","year":"2001","unstructured":"Washburn, M.P., Wolters, D. & Yates, J.R., III. Large-scale analysis of the yeast proteome by multdimensional protein identification technology. Nat. Biotechnol. 19, 242\u2013247 (2001).","journal-title":"Nat. Biotechnol."},{"key":"BFnmeth738_CR29","doi-asserted-by":"publisher","first-page":"664","DOI":"10.1002\/(SICI)1522-2683(19990101)20:4\/5<664::AID-ELPS664>3.0.CO;2-A","volume":"20","author":"M Quadroni","year":"1999","unstructured":"Quadroni, M. & James, P. Proteomics and automation. Electrophoresis 20, 664\u2013677 (1999).","journal-title":"Electrophoresis"},{"key":"BFnmeth738_CR30","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1038\/90754","volume":"19","author":"P Mitchell","year":"2001","unstructured":"Mitchell, P. Microfluidics \u2013 downsizing large-scale biology. Nat. Biotechnol. 19, 717\u2013721 (2001).","journal-title":"Nat. Biotechnol."},{"key":"BFnmeth738_CR31","doi-asserted-by":"publisher","first-page":"379","DOI":"10.1016\/S0165-9936(00)00013-3","volume":"19","author":"RD Oleschuk","year":"2000","unstructured":"Oleschuk, R.D. & Harrison, D.J. Analytical microdevices for mass spectrometry. Trends Anal. Chem. 19, 379\u2013388 (2000).","journal-title":"Trends Anal. Chem."},{"key":"BFnmeth738_CR32","doi-asserted-by":"publisher","first-page":"5213","DOI":"10.1021\/ac0300901","volume":"75","author":"J Astorga-Wells","year":"2003","unstructured":"Astorga-Wells, J., J\u00f6rnvall, H. & Bergman, T. A microfluidic electrocapture device in sample preparation for protein analysis by MALDI mass spectrometry. Anal. Chem. 75, 5213\u20135219 (2003).","journal-title":"Anal. Chem."},{"key":"BFnmeth738_CR33","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1016\/S0003-2697(03)00361-0","volume":"321","author":"E Caputo","year":"2003","unstructured":"Caputo, E., Moharram, R. & Martin, B.M. Methods for on-chip protein analysis. Anal. Biochem. 321, 116\u2013124 (2003).","journal-title":"Anal. Biochem."},{"key":"BFnmeth738_CR34","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1006\/jmbi.1998.1726","volume":"278","author":"MR Wilkins","year":"1998","unstructured":"Wilkins, M.R. et al. Protein identification with N- and C-terminal sequence tags in proteome projects. J. Mol. Biol. 278, 599\u2013608 (1998).","journal-title":"J. Mol. Biol."}],"container-title":["Nature Methods"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nmeth738.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nmeth738","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nmeth738.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T23:58:50Z","timestamp":1684454330000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nmeth738"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,2,17]]},"references-count":34,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,3]]}},"alternative-id":["BFnmeth738"],"URL":"https:\/\/doi.org\/10.1038\/nmeth738","relation":{},"ISSN":["1548-7091","1548-7105"],"issn-type":[{"value":"1548-7091","type":"print"},{"value":"1548-7105","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,2,17]]}}}