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Although the mechanisms by which MT poisons elicit mitotic arrest are fairly understood, efforts are still needed towards elucidating how cancer cells respond to antimitotic drugs owing to cytotoxicity and resistance side effects. Here, we identified the critical G2\/M transcription factor Forkhead box M1 (FOXM1) as a molecular determinant of cell response to antimitotics. We found FOXM1 repression to increase death in mitosis (DiM) due to upregulation of the BCL-2 modifying factor (<jats:italic>BMF<\/jats:italic>) gene involved in anoikis, an apoptotic process induced upon cell detachment from the extracellular matrix. FOXM1 binds to a <jats:italic>BMF<\/jats:italic> intronic cis-regulatory element that interacts with both the <jats:italic>BMF<\/jats:italic> and the neighbor gene <jats:italic>BUB1B<\/jats:italic> promoter regions, to oppositely regulate their expression. This mechanism ensures that cells treated with antimitotics repress <jats:italic>BMF<\/jats:italic> and avoid DiM when FOXM1 levels are high. In addition, we show that this mechanism is partly disrupted in anoikis\/antimitotics-resistant tumor cells, with resistance correlating with lower <jats:italic>BMF<\/jats:italic> expression but in a FOXM1-independent manner. These findings provide a stratification biomarker for antimitotic chemotherapy response.<\/jats:p>","DOI":"10.1038\/s41419-021-03822-5","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T14:03:18Z","timestamp":1621951398000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["FOXM1 repression increases mitotic death upon antimitotic chemotherapy through BMF upregulation"],"prefix":"10.1038","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9104-3254","authenticated-orcid":false,"given":"Sara","family":"Vaz","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9958-9238","authenticated-orcid":false,"given":"F\u00e1bio J.","family":"Ferreira","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3578-9120","authenticated-orcid":false,"given":"Joana C.","family":"Macedo","sequence":"additional","affiliation":[]},{"given":"Gil","family":"Leor","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7098-2378","authenticated-orcid":false,"given":"Uri","family":"Ben-David","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8591-7138","authenticated-orcid":false,"given":"Jos\u00e9","family":"Bessa","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8741-0744","authenticated-orcid":false,"given":"Elsa","family":"Logarinho","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2021,5,25]]},"reference":[{"key":"3822_CR1","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1038\/nrc1317","volume":"4","author":"MA Jordan","year":"2004","unstructured":"Jordan, M. 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