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Autoinhibited plasma membrane proton (H\n                    <jats:sup>+<\/jats:sup>\n                    ) ATPases (AHAs) have been proposed to energize pollen tube growth and underlie cell polarity, however, mechanistic evidence for this is lacking. Here we report that the combined loss of\n                    <jats:italic>AHA6, AHA8<\/jats:italic>\n                    , and\n                    <jats:italic>AHA9<\/jats:italic>\n                    in\n                    <jats:italic>Arabidopsis thaliana<\/jats:italic>\n                    delays pollen germination and causes pollen tube growth defects, leading to drastically reduced fertility. Pollen tubes of\n                    <jats:italic>aha<\/jats:italic>\n                    mutants had reduced extracellular proton (H\n                    <jats:sup>+<\/jats:sup>\n                    ) and anion fluxes, reduced cytosolic pH, reduced tip-to-shank proton gradients, and defects in actin organization. Furthermore, mutant pollen tubes had less negative membrane potentials, substantiating a mechanistic role for AHAs in pollen tube growth through plasma membrane hyperpolarization. Our findings define AHAs as energy transducers that sustain the ionic circuit defining the spatial and temporal profiles of cytosolic pH, thereby controlling downstream pH-dependent mechanisms essential for pollen tube elongation, and thus plant fertility.\n                  <\/jats:p>","DOI":"10.1038\/s41467-020-16253-1","type":"journal-article","created":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T06:03:18Z","timestamp":1589436198000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":111,"title":["Plasma membrane H+-ATPases sustain pollen tube growth and fertilization"],"prefix":"10.1038","volume":"11","author":[{"given":"Robert D.","family":"Hoffmann","sequence":"first","affiliation":[]},{"given":"Maria Teresa","family":"Portes","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4703-8068","authenticated-orcid":false,"given":"Lene Irene","family":"Olsen","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9911-2891","authenticated-orcid":false,"given":"Daniel Santa Cruz","family":"Damineli","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1704-8049","authenticated-orcid":false,"given":"Maki","family":"Hayashi","sequence":"additional","affiliation":[]},{"given":"Cust\u00f3dio O.","family":"Nunes","sequence":"additional","affiliation":[]},{"given":"Jesper T.","family":"Pedersen","sequence":"additional","affiliation":[]},{"given":"Pedro T.","family":"Lima","sequence":"additional","affiliation":[]},{"given":"Cl\u00e1udia","family":"Campos","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1100-5478","authenticated-orcid":false,"given":"Jos\u00e9 A.","family":"Feij\u00f3","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9982-6114","authenticated-orcid":false,"given":"Michael","family":"Palmgren","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2020,5,14]]},"reference":[{"key":"16253_CR1","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1387\/ijdb.052023lb","volume":"49","author":"L Boavida","year":"2005","unstructured":"Boavida, L., Becker, J. 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