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We present the 3.3\u2009\u00c5 resolution single-particle cryo-electron microscopy structure of <jats:italic>Mycobacterium smegmatis<\/jats:italic> EmbB, providing insights on substrate binding and reaction mechanism. Mutations that confer ethambutol resistance map mostly around the putative active site, suggesting this to be the location of drug binding.<\/jats:p>","DOI":"10.1038\/s41467-020-17202-8","type":"journal-article","created":{"date-parts":[[2020,7,7]],"date-time":"2020-07-07T10:04:08Z","timestamp":1594116248000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis"],"prefix":"10.1038","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6656-6320","authenticated-orcid":false,"given":"Yong Zi","family":"Tan","sequence":"first","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0829-9054","authenticated-orcid":false,"given":"Jos\u00e9","family":"Rodrigues","sequence":"additional","affiliation":[]},{"given":"James E.","family":"Keener","sequence":"additional","affiliation":[]},{"given":"Ruixiang Blake","family":"Zheng","sequence":"additional","affiliation":[]},{"given":"Richard","family":"Brunton","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0130-8739","authenticated-orcid":false,"given":"Brian","family":"Kloss","sequence":"additional","affiliation":[]},{"given":"Sabrina I.","family":"Giacometti","sequence":"additional","affiliation":[]},{"given":"Ana L.","family":"Ros\u00e1rio","sequence":"additional","affiliation":[]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4068-8092","authenticated-orcid":false,"given":"Michael","family":"Niederweis","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1876-196X","authenticated-orcid":false,"given":"Oliver B.","family":"Clarke","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8331-8211","authenticated-orcid":false,"given":"Todd L.","family":"Lowary","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8115-1772","authenticated-orcid":false,"given":"Michael T.","family":"Marty","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8419-5420","authenticated-orcid":false,"given":"Margarida","family":"Archer","sequence":"additional","affiliation":[]},{"given":"Clinton S.","family":"Potter","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0624-5020","authenticated-orcid":false,"given":"Bridget","family":"Carragher","sequence":"additional","affiliation":[]},{"given":"Filippo","family":"Mancia","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2020,7,7]]},"reference":[{"key":"17202_CR1","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1146\/annurev-micro-091014-104121","volume":"69","author":"M Jankute","year":"2015","unstructured":"Jankute, M., Cox, J. 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