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Although this mode of division was proposed over four decades ago, the molecular mechanism that ensures this geometry of division has remained elusive. Here we show, for three different strains, that <jats:italic>S. aureus<\/jats:italic> cells do not regularly divide in three alternating perpendicular planes as previously thought. Imaging of the divisome shows that a plane of division is always perpendicular to the previous one, avoiding bisection of the nucleoid, which segregates along an axis parallel to the closing septum. However, one out of the multiple planes perpendicular to the septum which divide the cell in two identical halves can be used in daughter cells, irrespective of its orientation in relation to the penultimate division plane. Therefore, division in three orthogonal planes is not the rule in <jats:italic>S. aureus<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/s41467-020-17940-9","type":"journal-article","created":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T12:22:04Z","timestamp":1597407724000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Reassessment of the distinctive geometry of Staphylococcus aureus cell division"],"prefix":"10.1038","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9151-5477","authenticated-orcid":false,"given":"Bruno M.","family":"Saraiva","sequence":"first","affiliation":[]},{"given":"Moritz","family":"Sorg","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9957-815X","authenticated-orcid":false,"given":"Ana R.","family":"Pereira","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5147-8659","authenticated-orcid":false,"given":"M\u00e1rio J.","family":"Ferreira","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5940-2990","authenticated-orcid":false,"given":"L\u00e9o C.","family":"Caulat","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1003-6376","authenticated-orcid":false,"given":"Nathalie T.","family":"Reichmann","sequence":"additional","affiliation":[]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7132-8842","authenticated-orcid":false,"given":"Mariana G.","family":"Pinho","sequence":"additional","affiliation":[]}],"member":"297","published-online":{"date-parts":[[2020,8,14]]},"reference":[{"key":"17940_CR1","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1038\/s41579-018-0147-4","volume":"17","author":"NA Turner","year":"2019","unstructured":"Turner, N. 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